本文選題：化學(xué)性腰交感神經(jīng)切除術(shù)(CLS) + COX_2�。� 參考：《新疆醫(yī)科大學(xué)》2005年碩士論文

【摘要】：目的:研究化學(xué)性腰交感神經(jīng)切除術(shù)(CLS)對直、結(jié)腸慢性刺激性炎癥和擴(kuò)張性傷害性刺激(CRD)的中樞保護(hù)作用,為臨床醫(yī)學(xué)提供基礎(chǔ)研究資料。方法:Wistar大鼠54只,隨機(jī)分為6組,每組9只,按二因素析因?qū)嶒?yàn)設(shè)置實(shí)驗(yàn)組,另外設(shè)立實(shí)驗(yàn)對照組和空白對照組。利用直、結(jié)腸慢性刺激性炎性致痛模型,切除模型大鼠的腰交感神經(jīng)后,運(yùn)用機(jī)械擴(kuò)張刺激觀察大鼠行為變化。用Miampamba評分方法改良后疼痛四級評分標(biāo)準(zhǔn)評分,重復(fù)3次取均值記錄結(jié)果。取間腦、腦橋、延髓、頸段(C_(5-8))、胸段(T_(3-6))、腰段(L_(1-3))、骶段(S_(2-4))脊髓連續(xù)切片后,延髓和脊髓用環(huán)氧合酶2(COX2),間腦、腦橋用膠質(zhì)纖維酸性蛋白(GFAP)免疫組化染色,顯微鏡下觀察相應(yīng)核團(tuán),計(jì)數(shù)陽性細(xì)胞數(shù)。結(jié)果:化學(xué)性腰交感神經(jīng)切除術(shù)CLS無論作為預(yù)防性手術(shù)還是治療性手術(shù)均能使中樞內(nèi)GFAP和COX2表達(dá)陽性的細(xì)胞數(shù)下降(p0.05)。NST,C_(5-8)中間帶和T_(3-6)中間帶外側(cè)核不是直、結(jié)腸慢性炎癥刺激和CRD擴(kuò)張性傷害刺激上傳通路中的中繼神經(jīng)元(p0.05)。一、二、三、四級反射各組比較直、結(jié)腸擴(kuò)張刺激閾值與手術(shù)順序無關(guān)而與手術(shù)有關(guān)(p0.05)。假手術(shù)不影響痛覺閾值(p0.05),而手術(shù)后痛覺閾值明顯上調(diào)(p0.05)。結(jié)論:化學(xué)性腰交感神經(jīng)切除術(shù)(CLS)在直、結(jié)腸慢性炎癥性刺激和CRD傷害刺激過程中能有效的保護(hù)中樞神經(jīng)系統(tǒng)。腰交感神經(jīng)可能是直、結(jié)腸傷害性刺激上傳的傳入神經(jīng)。CLS的中樞保護(hù)作用與手術(shù)時(shí)間順序無關(guān)。
[Abstract]:Objective: to study the central protective effect of chemical lumbar sympathectomy (CLS) on chronic irritant inflammation of colon and dilated nociceptive stimulation (CRD), and to provide basic data for clinical medicine. Methods Fifty-four Wistar rats were randomly divided into 6 groups with 9 rats in each group. The experimental group was set up according to the two-factor factorial experiment, and the experimental control group and the blank control group were set up. The model of pain induced by chronic irritation of colon and straight colon was used to observe the behavior of rats after the lumbar sympathetic nerve was excised and the mechanical dilatation was used to observe the behavior of the rats. The modified Miampamba scoring method was used to evaluate the four levels of pain. Diencephalon, pons, medulla oblongata, cervical segment (C5-8), thoracic segment (T3-6),) lumbar segment (L1-3), sacral segment (S2-4) spinal cord were sectioned continuously, the medulla oblongata and spinal cord were stained with cyclooxygenase-2 (COX2), diencephalon and pons were stained with glial fibrillary acidic protein (GFAP) immunohistochemical staining, and the corresponding nuclei were observed under microscope. Count the number of positive cells. Results: CLS could decrease the number of GFAP and COX2 positive cells in central nervous system (p0.05). NST-C5-8 and T3-6 medial zone were not straight. Relay neurons in the upload pathway of chronic inflammatory stimulation and CRD distensible nociceptive stimulation (p0.05). The first, second, third, and fourth reflex groups were straight, and the threshold of colon dilatation stimulation was not related to the operation order but to the operation (p0.05). The nociceptive threshold was not affected by sham operation (p 0.05), but increased significantly after operation (p 0.05). Conclusion: chemical lumbar sympathectomy (CLS) can effectively protect the central nervous system during chronic inflammatory stimulation and CRD nociceptive stimulation. Lumbar sympathetic nerve may be straight, the central protective effect of afferent nerve. CLS uploaded by colonic nociceptive stimulation is not related to the operation time sequence.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R361

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 董國祥,趙軍;化學(xué)性腰交感神經(jīng)切除術(shù)治療下肢缺血性疾病[J];北京醫(yī)科大學(xué)學(xué)報(bào);1998年01期

2 夏鋒,何振平,段恒春,李昆,陳莉,王小麗,董家鴻;去交感神經(jīng)狀態(tài)對肝部分切除后肝再生的影響[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2001年04期

3 云峰,周百中,曹玉文,萬琳;胸腔鏡交感神經(jīng)切除術(shù)9例報(bào)告[J];腹腔鏡外科雜志;2002年04期

4 向勇;交感神經(jīng)系統(tǒng)在炎癥痛中的作用[J];國外醫(yī)學(xué).麻醉學(xué)與復(fù)蘇分冊;2003年02期

5 董國祥;化學(xué)性腰交感神經(jīng)切除術(shù)治療下肢缺血性疾病的評價(jià)[J];臨床外科雜志;2001年02期

6 李康生,董菁,瘳傳紅,黃子亮;化學(xué)性交感神經(jīng)切除對免疫功能的影響[J];免疫學(xué)雜志;1997年01期

7 李震,陳軍;大鼠脊髓環(huán)氧合酶在皮下注射蜜蜂毒誘發(fā)的持續(xù)性自發(fā)痛、原發(fā)性痛敏的發(fā)生和在脊髓c-fos蛋白表達(dá)中的作用[J];神經(jīng)解剖學(xué)雜志;2001年02期

8 羅層,陳軍;膠質(zhì)細(xì)胞在病理性痛中的作用[J];神經(jīng)解剖學(xué)雜志;2002年04期

9 李震,陳軍;環(huán)氧合酶及其在病理性痛中的作用[J];神經(jīng)解剖學(xué)雜志;2003年01期

10 徐哠,陳勝國,甘子明,阿地力江·伊明,陳曉,陳蓉,古麗巴哈爾·尼牙孜,闈江利;大鼠直、結(jié)腸炎CLS的鎮(zhèn)痛作用及COX2與GFAP在CNS表達(dá)的形態(tài)學(xué)觀察[J];新疆醫(yī)科大學(xué)學(xué)報(bào);2005年04期

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