本文選題：一氧化氮 + 一氧化氮供體�。� 參考：《中國醫(yī)科大學》2005年碩士論文

【摘要】：目的 一氧化氮(NO)是近年發(fā)現(xiàn)的一個高活性氣體信號分子,除舒張血管外還是參與炎癥反應、感染性免疫調(diào)控活動與神經(jīng)傳導的重要介質(zhì)。NO供體是能釋放活性NO的化合物。研究發(fā)現(xiàn)NO具有明顯抑制細菌內(nèi)毒素引起的炎性反應過程。已知抗菌藥物對細菌內(nèi)毒素的清除及其誘發(fā)的炎性反應和組織損傷無效。如果重癥肺炎在使用抗菌藥物的同時,吸入NO氣體或霧化吸入NO供體,可能收到抗菌、抗炎的雙重效果。本研究目的在于探討NO及其供體SNAP的抑菌作用。 實驗一、不同濃度NO對五種病菌生長影響的體外實驗 方法 將表皮葡萄球菌、大腸桿菌、金黃色葡萄球菌、綠膿桿菌、白色念珠菌五種常見呼吸道致病菌稀釋為10~2CFU,按10μl/碟、50μl/碟,分別接種于MH瓊脂培養(yǎng)皿。分為空氣暴露組和10PPM、25PPM、40PPM NO暴露組。在溫度37℃,濕度45-60%的細菌孵育箱,培養(yǎng)24小時。取出,進行CFU計數(shù),并計算出平均抑菌率。 結果 1.10μl/碟、50μl/碟白色念珠菌暴露于NO濃度為10PPM、25PPM、40PPM中明顯抑制(P0.05)。 2.10μl/碟表皮葡萄球菌暴露于NO濃度為10PPM、25PPM、40PPM中明顯抑制(P0.05)。50μl/碟表皮葡萄球菌暴露于NO濃度為25PPM、
[Abstract]:Objective nitric oxide (no) is a highly active gas signaling molecule found in recent years. The important mediators of infectious immune regulation and nerve conduction. No donor is a compound that can release active no. It was found that no could inhibit the inflammatory reaction induced by bacterial endotoxin. Bacterial endotoxin clearance, inflammatory response and tissue damage induced by antimicrobial agents are not effective. If severe pneumonia inhale no gas or atomized no donor while using antimicrobial agents, it may have the dual effect of anti-bacterial and anti-inflammatory. The aim of this study was to investigate the bacteriostatic effect of no and its donor SNAP. Experiment 1. In vitro, Staphylococcus epidermidis was used to study the effects of different concentrations of no on the growth of five kinds of bacteria. Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans were diluted to 10 ~ 2 CFU. They were divided into air exposure group and 10 PPMN 25 PPMN 40 PPM no exposure group. The culture temperature was 37 鈩，

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