本文選題：Hedgehog + IPMN　； 參考：《第二軍醫(yī)大學》2007年碩士論文

【摘要】： 目的:利用SHH-N/pET22b重組大腸桿菌,誘導(dǎo)融合蛋白表達,免疫家兔制備多抗血清。檢測胰腺導(dǎo)管內(nèi)乳頭狀粘液性腫瘤(IPMN)組織中SHH的表達。 方法:SHH-N/pET22b重組大腸桿菌經(jīng)IPTG誘導(dǎo)表達,Western免疫印跡鑒定。融合蛋白免疫家兔制備多抗血清,免疫組化檢測IPMN組織中SHH的表達。 結(jié)果:成功誘導(dǎo)表達目的蛋白。SHH多抗血清經(jīng)Western免疫印跡鑒定。免疫組化檢測IPMN標本呈陽性表達。 結(jié)論:重組質(zhì)粒SHH-N/pET22b成功表達目的蛋白;并制備了SHH多抗血清,為研究Hedgehog信號通路在胰腺癌中的發(fā)病機制提供了有效工具。
[Abstract]:Aim: to induce the expression of fusion protein by Recombinant Escherichia coli SHH-N / pET22b and immunize rabbits to prepare polyclonal antiserum. The expression of SHH in pancreatic intraductal papillary mucinous tumor (IPMN) was detected. Methods the recombinant Escherichia coli was identified by Western blot after IPTG induced expression. The fusion protein was used to immunize rabbits to prepare polyantiserum and to detect the expression of SHH in IPMN tissue by immunohistochemistry. Results: the target protein. SHH polyantibodies were successfully induced and identified by Western blot. The positive expression of IPMN was detected by immunohistochemistry. Conclusion: the recombinant plasmid SHH-N / pET22b successfully expressed the target protein and prepared SHH polyantiserum, which provides an effective tool for studying the pathogenesis of Hedgehog signaling pathway in pancreatic cancer.
【學位授予單位】：第二軍醫(yī)大學
【學位級別】：碩士
【學位授予年份】：2007
【分類號】：R341;R735.9

【參考文獻】

相關(guān)期刊論文 前1條

1 周小鴿,王鵬,陸鳴,劉麗娜,張彥寧,張淑紅,陳光勇,張長淮,黃受方;加熱抗原修復(fù)對內(nèi)源性抗生物素蛋白結(jié)合物的影響及其對策[J];中華病理學雜志;2002年06期

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本文編號：2044620