本文選題：博爾納病病毒 + 感染��； 參考：《重慶醫(yī)科大學(xué)》2007年碩士論文

【摘要】： 第一部分重慶地區(qū)家兔博爾納病病毒自然感染狀況研究 目的:檢測重慶地區(qū)家兔博爾納病病毒(Borna Disease Virus ,BDV)隱性帶毒情況。分析該BDV的可能種系來源及其與標(biāo)準(zhǔn)株strain V/FR、strain He/80/FR和strain H1766的同源性。 方法:采用巢式逆轉(zhuǎn)錄酶PCR結(jié)合熒光定量PCR(Real-time nRT-PCR)檢測重慶地區(qū)60例健康家兔腦組織BDV P24基因片段,并對其中的陽性產(chǎn)物進(jìn)行基因序列測定,然后用BLAST軟件以及DNAstar V6.13 EditSeq軟件對測序結(jié)果進(jìn)行分析。 結(jié)果:2例家兔為陽性。該BDV P24片段的核苷酸序列與馬源BDV strain H1766株同源性最高,為97.67%,與標(biāo)準(zhǔn)株strain V/FR和strain He/80/FR同源性均為96.51%,但是它們編碼的氨基酸相同。 結(jié)論:重慶地區(qū)家兔中存在動物源性BDV隱性感染,該BDV P24核苷酸序列與strain V/FR和strain He/80/FR具有高度同源性。但是與馬源的strain H1766同源性最高。 第二部分重慶地區(qū)狗博爾納病病毒自然感染狀況的初步探討 目的:初步了解重慶地區(qū)狗BDV的隱性感染情況,對檢測到的陽性片段進(jìn)行核苷酸序列比對,探討其可能的種系來源及其與國外公認(rèn)的標(biāo)準(zhǔn)株strain V/FR、strain He/80/FR和strain H1766的同源性。 方法:采用Real-time nRT-PCR檢測重慶地區(qū)40例健康狗腦組織(右側(cè)前額葉)BDV P24基因片段,并對其中的陽性產(chǎn)物進(jìn)行基因序列測定,然后用BLAST軟件以及DNAstar V6.13 EditSeq軟件對測序結(jié)果進(jìn)行分析。 結(jié)果:5例狗為陽性。該BDV P24片段的核苷酸序列與標(biāo)準(zhǔn)株strain H1766和strain V/FR同源性最高,為97.67%。與strain He/80/FR的同源性為94.19%,與已有報(bào)道的奧地利狗源BDV同源性為95.35%。但是它們編碼的氨基酸相同。 結(jié)論:重慶地區(qū)狗中存在動物源性BDV的隱性感染,該BDV P24核苷酸序列與strain H1766、strain V/FR和strain He/80/FR具有高度同源性。 第三部分重慶地區(qū)鴿子博爾納病病毒自然感染狀況的研究 目的:初步了解重慶地區(qū)鴿子博爾納病病毒(Borna DiseaseVirus,BDV)隱性感染狀況,對其感染的病毒株進(jìn)行核苷酸序列比對,探討其可能的種系來源以及與國外公認(rèn)的標(biāo)準(zhǔn)株strain V/FR、strain He/80/FR和strain H1766的同源性 方法:本研究采用Real-time nRT-PCR技術(shù)對重慶地區(qū)60只鴿子的外周血單核細(xì)胞(PBMCs)中的BDV P24基因片段進(jìn)行了檢測,并且對陽性PCR產(chǎn)物進(jìn)行測序。然后用BLAST軟件以及DNAstar V6.13 EditSeq軟件對該片段進(jìn)行核苷酸比對及序列分析。 結(jié)果:四例樣本為陽性。該BDV P24片段的核苷酸序列與法國狐貍分離的BDV同源性最高,為97.67%。與標(biāo)準(zhǔn)株strain V/FR和strain He/80/FR的同源性都是96.51%,與strain H1766同源性僅為95.35%。但是它們編碼的氨基酸相同。 結(jié)論:本研究表明重慶地區(qū)鴿子存在博爾納病病毒的隱性感染,其感染病毒的P24核苷酸片段與strain V/FR、strain He/80/FR以及strain H1766等國際公認(rèn)的標(biāo)準(zhǔn)病毒株存在高度同源性。變異程度均小于4.65%,且其編碼的氨基酸無差別。
[Abstract]:A study on the natural infection status of Borna disease virus in the first part of Chongqing






Objective : To investigate the recessive herpes zoster virus ( BDV ) in rabbits with Borna Disease Virus ( BDV ) . The possible source of BDV and its homology with strain V / FR , strain He / 80 / FR and strain H1766 were analyzed .






Methods : The BDV P24 gene fragment of 60 healthy rabbits was detected by nested reverse transcriptase polymerase chain reaction ( Real - time nRT - PCR ) , and the positive products were sequenced . Then the sequencing results were analyzed by BLAST software and DNAstar V6 . 13 EditSeq software .






Results : The homology of nucleotide sequence of BDV P24 fragment was 97.67 % , which was 96.51 % and that of strain V / FR and strain He / 80 / FR was 96.51 % , but the amino acid sequence was the same as that of the standard strain V / FR and strain He / 80 / FR .






Conclusion : The BDV P24 nucleotide sequence is highly homologous to strain V / FR and strain He / 80 / FR in the rabbit of Chongqing . However , it has the highest homology with strain H1766 .






A Preliminary Study on the Natural Infection of Dog Borna Disease Virus in the Second Part of Chongqing






Objective : To investigate the recessive infection of BDV in Chongqing , and to investigate the nucleotide sequence alignment of the detected positive fragment , and explore its possible source of infection and its homology with other internationally recognized strain V / FR , strain He / 80 / FR and strain H1766 .






Methods : The BDV P24 gene fragment was detected by Real - time nRT - PCR in 40 healthy dog brain tissues ( right anterior frontal lobe ) , and the positive products were sequenced . Then the sequencing results were analyzed by BLAST software and DNAstar V6 . 13 EditSeq software .






Results : 5 cases of dogs were positive . The nucleotide sequence of BDV P24 fragment was the highest with strain H1766 and strain V / FR of strain H1766 and strain V / FR , which was 97.67 % .






Conclusion : The BDV P24 nucleotide sequence has high homology with strain H1766 , strain V / FR and strain He / 80 / FR .






A study on the natural infection status of pigeon Borna disease virus in the third part of Chongqing






Objective : To investigate the recessive infection status of Borna DiseaseVirus ( BDV ) in Chongqing region , to investigate the nucleotide sequence alignment of the infected virus strains , to explore the possible source of infection and to share the homology of strain V / FR , strain He / 80 / FR and strain H1766 .






Methods : The BDV P24 gene fragment in peripheral blood mononuclear cells ( PBMCs ) of 60 pigeons in Chongqing was detected by Real - time nRT - PCR , and the positive PCR products were sequenced . Then , the nucleotide alignment and sequence analysis were performed with BLAST software and DNAstar VB.13 EditSeq software .






Results : Four samples were positive . The nucleotide sequence of the BDV P24 fragment was up to 97.67 % . The homology with strain V / FR and strain He / 80 / FR was 96.51 % . The homology with strain H1766 was only 95.35 % , but the amino acids were the same .






Conclusion : This study shows that there is a recessive infection of Borna disease virus in the pigeon in Chongqing . The P24 nucleotide fragment of its infected virus has high homology with strain V / FR , strain He / 80 / FR and strain H1766 . The degree of variation is less than 4.65 % , and its encoded amino acid is no difference .
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R373

【引證文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 張英英;中國新疆和重慶地區(qū)多物種博爾納病病毒的檢測及種系發(fā)生分析[D];重慶醫(yī)科大學(xué);2010年

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本文編號：2043259