發(fā)布時間：2018-06-19 02:54

  本文選題：鉤端螺旋體 + invA基因�。� 參考：《四川大學》2005年博士論文

【摘要】：鉤體為廣泛流行的人獸共患病——鉤體病的病原體。目前,對鉤體的致病及免疫分子機理的認識不甚清楚。賴型鉤體56601株及哥本哈根株基因組測序表明,約60%的鉤體預測基因的功能未知。因此,鉤體的重要致病及免疫相關基因功能急需加強研究。 invA基因存在于致病的賴型鉤體56601株及哥本哈根株中,信息分析表明,該基因與巴爾通體內(nèi)的與編碼侵襲能力相關蛋白IalA的基因等具有很高的相似性;以PCR擴增試驗也證實,invA基因存在于強毒賴型鉤體017株中,但不存在于無毒鉤體Patoc Ⅰ株中。提示invA基因很可能為致病相關基因,同時,也很可能是一個免疫侯選基因及用于鉤體診斷的目的基因。 本研究選擇賴型鉤體017株中invA基因為目標,PCR擴增出含完整讀碼框的invA基因,與原核表達載體pET32a(+)連接構建重組表達載體pETINVA,在大腸桿菌BL21(DE3)中表達出分子量約為35kDa的InvA融
[Abstract]:Leptospira is the pathogen of zoonosis-leptospirosis. At present, the pathogenesis of leptospirosis and the mechanism of immune molecules are not well understood. Genomic sequencing of 56601 and Copenhagen Leptospira strains showed that about 60% of Leptospira predictive genes were unknown. Therefore, the important pathogenicity and immune-related gene function of Leptospira urgently need to be studied. InvA gene exists in 56601 strains of Leptospira and Copenhagen strain. There was a high similarity between the gene and the gene encoding invasion ability related protein IalA in Balton, and the PCR amplification test also confirmed that the inva gene existed in the highly virulent Leptospira Leptospira strain 017, but not in the non-toxic Leptospira Patoc 鈪，

本文編號：2038066