發(fā)布時(shí)間：2018-06-16 01:14

  本文選題：高原 + 低氧�。� 參考：《第一軍醫(yī)大學(xué)》2005年博士論文

【摘要】：目的:研究低氧及低氧復(fù)合運(yùn)動(dòng)情況下大鼠體重、骨骼肌顯微、超微結(jié)構(gòu)及低氧誘導(dǎo)因子發(fā)生變化的規(guī)律,探討在低氧條件下提高機(jī)體低氧習(xí)服能力的途徑和可能的機(jī)制。 方法:分別建立大鼠低氧及正常氧情況下運(yùn)動(dòng)模型:雄性SD大鼠80只,隨機(jī)分為4組,即常氧安靜組(A)、常氧運(yùn)動(dòng)組(B)、低氧安靜組(C)、低氧運(yùn)動(dòng)組(D)。大鼠運(yùn)動(dòng)采用跑臺(tái)模型,速度設(shè)定為25m/min,檢測(cè)在第3天(急性低氧)、第7天、第10天與第14天(慢性低氧)各組大鼠體重(W)變化,光鏡和電鏡分別觀察骨骼肌顯微和超微結(jié)構(gòu)的改變,對(duì)骨骼肌中低氧誘導(dǎo)因子-1(HIF-1)的表達(dá)運(yùn)用實(shí)時(shí)熒光PCR(fluorescence quantitative PCR FQ-PCR)、表面加強(qiáng)激光解析電離化芯片技術(shù)(Surface-enhanced LaserDesorption/Ionization SELDI)進(jìn)行定量分析;采用2×4×2析因分析,分組變量分別為氧含量、運(yùn)動(dòng)與否以及時(shí)間,其中氧含量為2個(gè)水平:正常氧和低氧;運(yùn)動(dòng)情況有2個(gè)水平:安靜與運(yùn)動(dòng);時(shí)間有4個(gè)水平,分別為3天、7天、10天、14天。分別研究低氧及運(yùn)動(dòng)的不同階段對(duì)骨骼肌形態(tài)結(jié)構(gòu)的影響以及骨骼肌中低氧誘導(dǎo)因子表達(dá)的變化規(guī)律,分析其相互作用,探討骨骼肌結(jié)構(gòu)、功能改變的發(fā)生機(jī)制及演變過(guò)程,尋找在低氧條件下促進(jìn)低氧習(xí)服能力的途徑,探討其發(fā)生的可能的機(jī)制。 結(jié)果:單純低氧組大鼠體重在試驗(yàn)前后有所降低,而正常氧安靜組、常氧運(yùn)動(dòng)組及低氧運(yùn)動(dòng)組大鼠體重均有不同程度的增加,與低氧安靜組比較有顯著意義(p0.05)。在肌肉形態(tài)學(xué)方面,單純低氧組大鼠急性期光鏡下肌纖維形態(tài)尚正常,電鏡下已出現(xiàn)線粒體體積增大、腫脹,嵴稍腫脹、嵴內(nèi)腔擴(kuò)大,嵴紊亂;在慢性期光鏡下出現(xiàn)肌纖維萎縮,電鏡下可見(jiàn)線粒體輕度腫脹,水性變及空泡化,
[Abstract]:Aim: to study the changes of body weight, skeletal muscle microstructure, ultrastructure and hypoxia inducible factors in hypoxic and hypoxic combined exercise rats, and to explore the ways and possible mechanisms of enhancing hypoxic acclimatization under hypoxic conditions. Methods: 80 male Sprague-Dawley (SD) rats were randomly divided into four groups: normal oxygen quiet group, normoxic exercise group, hypoxia quiet group and hypoxic exercise group. The rats used a treadmill model with a speed of 25 m / min. The changes of body weight were measured on day 3 (acute hypoxia, day 7, day 10 and day 14 (chronic hypoxia). The changes of microstructure and ultrastructure of skeletal muscle were observed by light microscope and electron microscope respectively. The expression of hypoxia inducible factor-1 (HIF-1) in skeletal muscle was quantitatively analyzed by real-time fluorescence quantitative FQ-PCRN, surface-enhanced laser desorption / ionization chip technique (Surface-enhanced LaserDesorptionIonization SELDI). Using 2 脳 4 脳 2 factorial analysis, the grouped variables are oxygen content, exercise or not, and time, in which oxygen content is 2 levels: normal oxygen level and hypoxia level; exercise condition has 2 levels: quiet and exercise; time has 4 levels. They were 3 days, 7 days, 10 days and 14 days. The effects of hypoxia and exercise on skeletal muscle morphology and the expression of hypoxia inducible factor in skeletal muscle were studied, and the mechanism and evolution of skeletal muscle structure and function were discussed. To find ways to promote hypoxic acclimatization under hypoxia conditions and explore the possible mechanism of its occurrence. Results: the body weight of rats in hypoxic group was decreased before and after the experiment, but the body weight of rats in normal oxygen quiet group, normoxic exercise group and hypoxic exercise group increased in varying degrees, which was significantly higher than that in hypoxic quiet group (P 0.05). In terms of muscle morphology, the morphology of muscle fibers was normal under light microscope in the acute phase of hypoxic rats. Mitochondria volume increased, swelling, cristae slightly swollen, cristal cavity enlarged and cristal disorder appeared under electron microscope. Myofibril atrophy was observed under the light microscope in chronic phase, slight swelling of mitochondria, waterborne degeneration and vacuolation were observed under electron microscope.
【學(xué)位授予單位】：第一軍醫(yī)大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2005
【分類號(hào)】：R363;R87

【引證文獻(xiàn)】

中國(guó)期刊全文數(shù)據(jù)庫(kù) 前1條

1 謝康玲;劉遂心;蔡穎;張文亮;;長(zhǎng)期中等強(qiáng)度運(yùn)動(dòng)對(duì)小鼠骨骼肌HIF-1α mRNA的表達(dá)及葡萄糖轉(zhuǎn)運(yùn)的影響[J];中國(guó)康復(fù)醫(yī)學(xué)雜志;2012年06期

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本文編號(hào)：2024528