本文選題：HHK + 坐骨神經(jīng)��； 參考：《第一軍醫(yī)大學(xué)》2005年博士論文

【摘要】：周圍神經(jīng)損傷和缺損的治療是顯微外科的難題之一。不少學(xué)者從不同角度,采用多種方法進(jìn)行了有益的探索,取得了很大的進(jìn)展。近年來,隨著組織工程學(xué)的興起,對周圍神經(jīng)缺損的修復(fù)探索出一條解決問題的捷徑。研制可生物降解的人工神經(jīng)套管,將此套管植入缺損神經(jīng)的近端和遠(yuǎn)端之間,將兩端神經(jīng)連接起來,在它們之間構(gòu)建一個有利于神經(jīng)再生的“微環(huán)境”,誘導(dǎo)損傷神經(jīng)的再生與修復(fù),將是很有前途的方法。目前,用于套接的材料很多,分兩大類,一類是合成材料,另一類是天然材料。勞杰等證明用硅膠管,并輔以施萬細(xì)胞,能誘導(dǎo)正中神經(jīng)再生。硅膠管有制備方便、不塌陷的優(yōu)點,但術(shù)后會產(chǎn)生致命的神經(jīng)卡壓現(xiàn)象及炎性刺激,另外需要再次手術(shù)取出,所以并不適合臨床應(yīng)用。靜脈管壁做為套管容易塌陷,而且其中的成纖維細(xì)胞易形成疤痕,阻礙神經(jīng)生長。膠原膜套管具有排異反應(yīng)小,可吸收、存儲方便的特點,適合臨床應(yīng)用。王光林等以雪旺細(xì)胞與PLA構(gòu)建組織工程化人工神經(jīng),修復(fù)周圍神經(jīng)缺損的效果接近于自體神經(jīng)移植。 我校自1991年以來,采用經(jīng)序貫生物化學(xué)特殊處理的人發(fā)角蛋白(humanhair keratin,HHK)為醫(yī)學(xué)生物材料,成功研制出HHK人工腱。經(jīng)動物實驗與臨床應(yīng)用證明,該人工腱不僅具有其它傳統(tǒng)醫(yī)用材料的生物學(xué)特性,而且還具有以下特點:組織相容性好;可完全降解吸收,并能誘導(dǎo)形成具有正常功能的自體腱形成,從而表明HHK可以作為一種新型的醫(yī)用生物材料應(yīng)用于臨床。
[Abstract]:The treatment of peripheral nerve injury and defect is one of the difficult problems in microsurgery. Many scholars have made great progress by using various methods from different angles. In recent years, with the rise of tissue engineering, the repair of peripheral nerve defects to explore a shortcut to solve the problem. A biodegradable artificial nerve cannula was developed. The cannula was implanted between the proximal and distal ends of the defective nerve, and the two ends of the nerve were connected to form a "microenvironment" conducive to nerve regeneration. It is a promising method to induce nerve regeneration and repair. At present, there are many kinds of materials for socket, one is synthetic material, the other is natural material. Lauger et al proved that the median nerve regeneration could be induced by silica gel tube supplemented with Schwann cells. Silicone tube has the advantages of convenient preparation and not collapse, but it will produce fatal nerve compression and inflammatory stimulation after operation, and need to be removed again, so it is not suitable for clinical application. The venous wall is prone to collapse as a cannula, and the fibroblasts are prone to scar formation, hindering nerve growth. Collagen membrane cannula is suitable for clinical application because of its advantages of small rejection, absorbability and convenient storage. Wang Guanglin used Schwann cells and PLA to construct tissue engineered artificial nerve, and the effect of repairing peripheral nerve defect was similar to that of autogenous nerve transplantation. Since 1991, HHK artificial tendon was successfully developed using human hair keratin HHKK (human hair keratin) treated by sequential biochemistry as medical biomaterial. Animal experiments and clinical applications have proved that the artificial tendon not only has the biological characteristics of other traditional medical materials, but also has the following characteristics: good histocompatibility, complete degradation and absorption. It can induce the formation of autologous tendon with normal function, which indicates that HHK can be used as a new medical biomaterial in clinic.
【學(xué)位授予單位】：第一軍醫(yī)大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2005
【分類號】：R322

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本文編號：2012133