發(fā)布時(shí)間：2018-06-10 07:11

  本文選題：樹突狀細(xì)胞 + 調(diào)節(jié)性T細(xì)胞�。� 參考：《吉林大學(xué)》2006年碩士論文

【摘要】：90年代中期以來,大量研究發(fā)現(xiàn)一類具有免疫調(diào)節(jié)功能的細(xì)胞亞群,如CD4+CD25+T細(xì)胞、Tr1細(xì)胞和Th3細(xì)胞等[1-3],盡管這些細(xì)胞的來源和分化目前還不是很明確,但是根據(jù)其功能特點(diǎn)可將其統(tǒng)稱為調(diào)節(jié)T細(xì)胞(regulatory T cell,Tr)。人和嚙齒類動(dòng)物體內(nèi)已經(jīng)成功分離純化出Tr細(xì)胞,其抑制或調(diào)節(jié)功能在體內(nèi)外實(shí)驗(yàn)中得到證實(shí)。因Tr細(xì)胞在感染免疫、腫瘤免疫及器官移植耐受的誘導(dǎo)中具有廣泛的應(yīng)用前景,目前已成為免疫耐受研究中的一個(gè)新熱點(diǎn)。 本研究利用體外培養(yǎng)人外周血單核細(xì)胞來源的未成熟樹突狀細(xì)胞(immature dendritic cell, iDC)誘導(dǎo)臍血淋巴細(xì)胞產(chǎn)生Tr細(xì)胞,經(jīng)流式細(xì)胞儀測定其細(xì)胞表型及細(xì)胞內(nèi)細(xì)胞因子,應(yīng)用MTT法測定誘導(dǎo)細(xì)胞的生物學(xué)功能,為進(jìn)一步探討未成熟DC誘導(dǎo)免疫耐受的機(jī)制奠定基礎(chǔ)。 結(jié)果表明:細(xì)胞因子聯(lián)合誘導(dǎo)人外周血單核細(xì)胞可獲得較大量、純度較高的不同發(fā)育階段的DC。未成熟DC與臍血淋巴細(xì)胞混合培養(yǎng)可誘導(dǎo)Tr細(xì)胞的生成,其細(xì)胞內(nèi)IL-10的分泌量明顯增加,CD4/CD25分子的表達(dá)與陰性對照組相比無統(tǒng)計(jì)學(xué)差異。經(jīng)混合淋巴細(xì)胞反應(yīng)發(fā)現(xiàn)Tr能抑制同種異體淋巴細(xì)胞的增殖以及降低細(xì)胞毒性T細(xì)胞(CTL)的抗腫瘤效應(yīng)。 本研究意義在于:我們通過建立的DC體外培養(yǎng)體系,誘導(dǎo)Tr細(xì)胞,為Tr細(xì)胞在感染免疫、腫瘤免疫和移植耐受的深入研究和臨床應(yīng)用提供了理論依據(jù)。
[Abstract]:Since the mid-1990s, a large number of studies have found a class of immunomodulatory cell subsets, such as CD4 CD25 T cells, Tr1 cells and Th3 cells [1-3], although the origin and differentiation of these cells are not yet clear. However, according to its functional characteristics, it can be called regulatory T cell Trus. Tr cells have been successfully isolated and purified from human and rodent, and their inhibitory or regulatory functions have been confirmed in vitro and in vivo. Tr cells may be widely used in the induction of infection immunity, tumor immunity and organ transplantation tolerance. In this study, immature dendritic cells (DCs) derived from human peripheral blood monocytes (PBMC) were used to induce Tr cells from cord blood lymphocytes. The cell phenotypes and intracellular cytokines were measured by flow cytometry, and the biological functions of induced cells were determined by MTT assay. The results showed that the combination of cytokines induced human peripheral blood mononuclear cells (PBMC) to obtain a large number of DCs with high purity at different developmental stages. Immature DC and umbilical cord blood lymphocytes co-cultured could induce Tr cells to produce Tr cells. The secretion of IL-10 increased significantly the expression of CD4 / CD25 molecules in immature DCs and umbilical cord blood lymphocytes was not significantly different from that in negative control group. Tr can inhibit the proliferation of allogeneic lymphocytes and reduce the antitumor effect of cytotoxic T cell line CTL. The significance of this study is that we can induce Tr cells through the established DC culture system in vitro. It provides a theoretical basis for the further study and clinical application of Tr cells in infection immunity, tumor immunity and transplantation tolerance.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R392

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