本文選題：金屬蛋白酶 + 解整連蛋白�。� 參考：《吉林大學(xué)》2005年碩士論文

【摘要】：提取中國長白山烏蘇里蝮蛇毒腺mRNA,逆轉(zhuǎn)錄合成cDNA,并以其為模板,進(jìn)行PCR 擴(kuò)增,PCR 產(chǎn)物克隆入pGEM-T Easy 載體并進(jìn)行了序列分析。結(jié)果顯示,烏蘇里蝮蛇前金屬蛋白酶原基因開框讀碼序列由1434bp 組成,編碼478 個(gè)氨基酸。由核苷酸順序推導(dǎo)的氨基酸序列可以看出,前金屬蛋白酶原基因最初的翻譯產(chǎn)物是酶原前體;依次含有18 氨基酸組成的信號(hào)肽,171 個(gè)氨基酸組成的酶原區(qū)和由289 個(gè)氨基酸組成的前金屬蛋白酶原基因(200 個(gè)氨基酸組成的金屬蛋白酶結(jié)構(gòu)域、16 個(gè)氨基酸組成的間隔區(qū)和73 個(gè)氨基酸組成的解整合蛋白結(jié)構(gòu)域)。烏蘇里蝮蛇前金屬蛋白酶原的金屬蛋白酶結(jié)構(gòu)域含有3 對(duì)二硫鍵;解整合蛋白結(jié)構(gòu)域含有6 對(duì)二硫鍵和特征性RGD(精氨酸-甘氨酸-天冬氨酸)結(jié)構(gòu)。其基因序列和結(jié)構(gòu)域組成與GenBank 中蛇毒金屬蛋白酶/解整合蛋白呈現(xiàn)高度同源性屬于PⅡ。氨基酸序列blast 比對(duì)發(fā)現(xiàn),酶原區(qū)和解整鏈蛋白結(jié)構(gòu)域呈現(xiàn)極高的同源性,而金屬蛋白酶結(jié)構(gòu)域卻出現(xiàn)了極高的變異,推測(cè)這些變異結(jié)構(gòu)區(qū)是為了適應(yīng)不同的底物、不同受體或同一受體的不同結(jié)構(gòu)域。
[Abstract]:MRNAs were extracted from the venom gland of Agkistrodon halys in Changbai Mountain, China, and were synthesized by reverse transcription. The PCR products were amplified by PCR and cloned into pGEM-T Easy vector and sequenced. The results showed that the open frame reading sequence of prometalloproteinase gene of Agkistrodon halys was composed of 1434bp and encoded 478 amino acids. The amino acid sequence deduced from nucleotide sequence shows that the original translation product of prometalloproteinase gene is proenzyme precursor. An 18-amino acid signal peptide composed of 171 amino acids in turn and a prometalloproteinase gene consisting of 289 amino acids, composed of 200 amino acids, composed of 16 amino acids. Domain and 73 amino acid composition of the unintegrated protein domain. The metalloproteinase domain of Agkistrodon halys Pallas has 3 pairs of disulfide bonds and 6 pairs of disulfide bonds and characteristic RGD (arginine glycine aspartic acid) structure. Its gene sequence and domain composition were highly homologous to the snake venom metalloproteinase / integrin in GenBank, belonging to P 鈪，

本文編號(hào)：1977126