胃粘膜中幽門螺桿菌與化生性病變的分布
發(fā)布時(shí)間:2018-06-04 04:53
本文選題:幽門螺桿菌 + 腸上皮化生 ; 參考:《延邊大學(xué)》2006年碩士論文
【摘要】:目的 探討胃黏膜中幽門螺桿菌(Hp)與化生性病變的分布及其相關(guān)性。 方法 選擇10例有Hp感染的手術(shù)切除胃標(biāo)本(胃或十二指腸潰瘍5例,癌癥5例),行全胃取材。每個(gè)蠟快行連續(xù)切片做(1) 蘇木素-伊紅染色,(2) Hp免疫組化染色,(3) 雙重染色;利用human gastric mucin(M1)抗體做免疫組化染色后再染alcJan blue(AB),把所有染色結(jié)果畫在取材圖上: 結(jié)果 根據(jù)(1) Hp,(2) 完全性腸上皮化生(IM),(3) 不完全性腸上皮化生(4)AB陽性的假幽門腺化生和/或頸黏液細(xì)胞(PM)的分布結(jié)果,,全部病例分為3型。Ⅰ型:以PM為主,Hp與化生性病變的分布呈正相關(guān),Ⅱ型和Ⅲ型:以IM為主,與Hp與化生性病變的分布呈負(fù)相關(guān);Ⅰ型5例中4例在不完全性腸上皮化生細(xì)胞見Hp感染:Ⅱ型和Ⅲ型的炎癥細(xì)胞浸潤及萎縮程度比Ⅰ型重:Ⅱ型和Ⅲ型5例均是癌癥病例,Ⅰ型5例中僅有1例是癌癥病例,其余4例是潰瘍病例。 結(jié)論:Ⅰ型胃黏膜中的PM和伴有Hp感染的IM,可能與誘發(fā)潰瘍性病變有關(guān),Ⅱ和Ⅲ型的IM,加上嚴(yán)重的炎癥持續(xù),可能增加癌發(fā)生的危險(xiǎn)性。
[Abstract]:Objective to investigate the distribution and correlation between Helicobacter pylori (HP) and metaplastic lesions in gastric mucosa. Methods 10 cases of gastric specimens with HP infection (5 cases of gastric or duodenal ulcer and 5 cases of cancer) were selected for total gastric sampling. (1) Hematin-eosin staining 2) Hp immunohistochemical staining / 3) double staining; using human gastric mucin M1) antibody for immunohistochemical staining and then staining for alcJan blue ABN, and drawing all the staining results on the drawing map: Results according to the distribution of complete intestinal metaplasia 3) incomplete intestinal metaplasia 4A AB positive pseudopyloric gland metaplasia and / or cervical mucus cell PMMC, All the cases were divided into 3 types: type 鈪
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