本文選題：ICOS(Inducible + co-stimulator�。� 參考：《蘇州大學(xué)》2005年碩士論文

【摘要】：本課題嘗試建立ICOS轉(zhuǎn)基因小鼠模型,將其應(yīng)用于日本血吸蟲病的研究。我們采用基因工程方法克隆人的全長ICOS基因并構(gòu)建轉(zhuǎn)基因載體,經(jīng)顯微注射和胚胎移植成功建立了ICOS轉(zhuǎn)基因小鼠模型,并對其生物學(xué)特性進行了初步研究,發(fā)現(xiàn)轉(zhuǎn)基因小鼠繁殖能力和生長曲線與正常FVB小鼠(野生型)沒有顯著差異,脾臟、胸腺等免疫器官也無顯著異常。轉(zhuǎn)基因小鼠感染日本血吸蟲后,檢測相關(guān)的細(xì)胞因子和血清抗體,同時觀察肝臟肉芽腫病變,結(jié)果表明,轉(zhuǎn)基因小鼠體內(nèi)免疫應(yīng)答較野生型小鼠強烈,而且Th2反應(yīng)更為強烈,提示共刺激分子ICOS可能在日本血吸蟲特異性Th1/Th2免疫偏移及其免疫病理機制中發(fā)揮重要作用。本研究為進一步了解ICOS的免疫調(diào)節(jié)功能,深入闡明日本血吸蟲病免疫致病的分子機制提供了一個潛在的模型工具,對控制蟲卵肉芽腫病變,防止晚期血吸蟲病的發(fā)生、發(fā)展具有重要的理論意義和應(yīng)用價值。
[Abstract]:In this paper, we try to establish ICOS transgenic mice model and apply it to schistosomiasis japonica. The full-length ICOS gene of human was cloned by genetic engineering method and the transgenic vector was constructed. The ICOS transgenic mouse model was successfully established by microinjection and embryo transfer, and its biological characteristics were preliminarily studied. It was found that there was no significant difference in reproductive ability and growth curve between transgenic mice and normal FVB mice (wild type), and there was no significant abnormality in immune organs such as spleen and thymus. After the transgenic mice were infected with Schistosoma japonicum, the related cytokines and serum antibodies were detected and liver granulomatous lesions were observed. The results showed that the immune response of transgenic mice was stronger than that of wild-type mice, and the Th2 reaction was stronger in transgenic mice than in wild-type mice. The results suggest that the costimulatory molecule ICOS may play an important role in Schistosoma japonicum specific Th1/Th2 immune migration and its immunopathological mechanism. This study provides a potential model tool for further understanding the immunomodulatory function of ICOS and elucidating the molecular mechanism of immune pathogenesis of schistosomiasis japonicum, which can control egg granuloma and prevent the occurrence of advanced schistosomiasis. Development has important theoretical significance and application value.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R-332
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本文編號：1975331