發(fā)布時(shí)間：2018-06-01 18:50

  本文選題：重組人促紅細(xì)胞生成素 + 腎臟��； 參考：《瀘州醫(yī)學(xué)院》2006年碩士論文

【摘要】：目的:本研究采用大鼠腎缺血再灌注損傷模型,探討重組人促紅細(xì)胞生成素(recombinant human erythropoietin, rHuEPO)對(duì)大鼠腎缺血再灌注損傷的作用,并對(duì)其可能機(jī)制進(jìn)行初步研究。方法:雄性Wistar大鼠72只,隨機(jī)分為三組:(1)假手術(shù)組(N=24),按取材時(shí)間不同又分為4個(gè)小組,即相當(dāng)于再灌注1h、6h、12h、24h取材組,每組6只;(2)腎缺血再灌注損傷模型組(N=24),組內(nèi)又分為再灌注1h、6h、12h、24h 4個(gè)時(shí)間點(diǎn),每個(gè)時(shí)間點(diǎn)6只;(3) rHuEPO組(N=24),組內(nèi)又分為再灌注1h、6h、12h、24h 4個(gè)時(shí)間點(diǎn),每個(gè)時(shí)間點(diǎn)6只。將動(dòng)物麻醉,開腹,假手術(shù)組切除右腎,不夾閉左腎動(dòng)脈,模型組和rHuEPO處理組切除右腎,夾閉左腎動(dòng)脈45min后松夾再灌,rHuEPO處理組在再灌注開始前尾靜脈注射rHuEPO3000U/Kg,假手術(shù)組和模型組在再灌注開始前尾靜脈注射等量生理鹽水。再灌注達(dá)相應(yīng)時(shí)間點(diǎn)時(shí),從各小組大鼠下腔靜脈取血3ml備測血尿素氮(BUN)和血肌酐(Cr),然后迅速切除左腎,稱取0.5克腎組織迅速冷凍,待作成10%組織勻漿,測定丙二醛(MDA)、超氧化物歧化酶(SOD)和髓過氧化物酶(MPO),其余腎組織用10%甲醛固定,制備石蠟切片,作HE染色觀察病理變化以及作免疫組化分析腎組織P-選擇素和ICAM-1表達(dá)情況。結(jié)果:(1)腎功能改變:模型組除再灌注1h組血BUN無明顯升高外,其余各小組BUN、Cr較假手術(shù)組明顯升高(P0.01),rHuEPO處理組除再灌注1h組BUN與假手術(shù)組無明顯差異外,其余各小組BUN較相應(yīng)時(shí)間點(diǎn)假手術(shù)組明顯升高(P0.05,P0.01),rHuEPO處理組中再灌注1h,12h,24h組血Cr較假手術(shù)組明顯升高(P0.05,P0.01),而再灌注6h組血Cr與假手術(shù)組無顯著差異。與腎IRI模型組比較,rHuEPO處理組再灌注6h、12h、24h組BUN、Cr明顯降低(P0.01,P0.05),rHuEPO處理后再灌注1h組血BUN和Cr與模型組無明顯差異。(2)腎組織生化指標(biāo)檢測結(jié)果:腎組織MDA、SOD測定結(jié)果:與假手術(shù)組比較,模型組各組腎組織MDA含量均明顯升高(P0.05,P0.01),而各組腎組織SOD活力均顯著降低(P0.05,P0.01),rHuEPO處理組除再灌注24h組MDA含量較假手術(shù)
[Abstract]:Aim: to investigate the effect of recombinant human erythropoietin (human erythropoietin, rHuEPO) on renal ischemia-reperfusion injury in rats and its possible mechanism. Methods: Seventy-two male Wistar rats were randomly divided into three groups. The sham operation group was divided into 4 groups according to the time of sampling. Each group (n = 6) was divided into 4 time points (1 h, 6 h, 12 h, 24 h) rHuEPO group (n = 6, n = 6) were divided into two groups (n = 6, n = 6). Animals were anesthetized, laparotomy, sham-operation group, right kidney was resected, left renal artery was not clipped, right kidney was resected in model group and rHuEPO treatment group. RHuEPO treatment group was injected with rHuEPO _ 3000U / kg before reperfusion, and the sham operation group and model group received the same amount of normal saline before reperfusion. Blood urea nitrogen bun (3ml) and creatinine (Cr) were collected from the inferior vena cava of the rats at the corresponding time point of reperfusion, and then the left kidney was quickly removed, and the kidney tissue was quickly frozen, and 10% tissue homogenate was prepared. The levels of malondialdehyde (MDA), superoxide dismutase (SOD) and myeloperoxidase (MPO) were measured. The other renal tissues were fixed with 10% formaldehyde to prepare paraffin sections. The pathological changes were observed by HE staining and the expression of P- selectin and ICAM-1 in renal tissues were analyzed by immunohistochemistry. Results (1) Renal function changes: except for 1 h reperfusion group, the blood BUN in the model group was significantly higher than that in the sham-operation group, except that the BUN in the model group was not significantly different from that in the sham-operation group, except that the BUN in the model group was significantly higher than that in the sham-operation group. The BUN of the other groups was significantly higher than that of the sham operation group at the corresponding time point. The serum Cr in the reperfusion group was significantly higher than that in the sham-operation group at 1h, 12h and 24h after reperfusion, but there was no significant difference between the six h reperfusion group and the sham-operation group. Compared with the renal IRI model group, the blood BUN and Cr in the rHuEPO treated group were significantly lower than those in the model group after reperfusion for 6 h and 12 h and 24 h after reperfusion. There was no significant difference in serum BUN and Cr between the model group and the model group. The results showed that: compared with the sham operation group, the blood BUN and Cr in the renal tissue of the RHuEPO treated group were significantly lower than those in the sham operation group, and the results were as follows: compared with the sham operation group, there was no significant difference in serum BUN and Cr between the model group and the model group. The content of MDA in renal tissue of model group was significantly higher than that of sham operation group, while the activity of SOD in renal tissue of each group was significantly lower than that of sham operation group except for 24 hours after reperfusion.
【學(xué)位授予單位】：瀘州醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R363

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 曾志磊,婁季宇,苗旺;促紅細(xì)胞生成素對(duì)大鼠腦缺血再灌注損傷后缺血側(cè)皮層神經(jīng)元凋亡和Bcl-2表達(dá)的影響[J];實(shí)用神經(jīng)疾病雜志;2005年02期

2 平蕾,沈霞,耿德勤,雒東江,徐凱,王梅申;促紅細(xì)胞生成素對(duì)大鼠局灶性腦缺血CAPSASE-3蛋白的影響[J];神經(jīng)疾病與精神衛(wèi)生;2004年05期

3 王硯青,江時(shí)森,樂美兆,邱紅;促紅細(xì)胞生成素對(duì)大鼠心肌缺血-再灌注損傷的保護(hù)作用[J];醫(yī)學(xué)研究生學(xué)報(bào);2005年04期

4 林曉英;黃濤;李中昕;李玉華;黃華;;促紅細(xì)胞生成素對(duì)大鼠腦缺血再灌注后半胱氨酸天冬酶-9 mRNA表達(dá)的影響[J];臨床神經(jīng)病學(xué)雜志;2005年06期

5 周同,李曉,宋巍,吳佩,張東華,張明均,陳楠,董德長;粘附分子在缺血再灌注腎損傷中的作用及阻斷意義[J];上海醫(yī)學(xué);2001年02期

6 喬淑紅,遲煥芳;促紅細(xì)胞生成素對(duì)視網(wǎng)膜缺血再灌注神經(jīng)元凋亡和caspase-2表達(dá)的影響[J];神經(jīng)解剖學(xué)雜志;2005年05期

7 夏安周,邢淑華,張昭輝;大鼠腎缺血再灌注過程中脂質(zhì)過氧化損傷的實(shí)驗(yàn)研究[J];徐州醫(yī)學(xué)院學(xué)報(bào);2004年02期

8 李燦,陳瑛,洪明玉;促紅細(xì)胞生成素預(yù)處理對(duì)大鼠急性缺血再灌注腎的保護(hù)作用[J];中國病理生理雜志;2004年12期

9 吳天順,任先軍,滕曉華,呂根法,張松濤,李杰,劉永才;促紅細(xì)胞生成素對(duì)脊髓缺血再灌注損傷保護(hù)作用及其機(jī)理的實(shí)驗(yàn)研究[J];中國矯形外科雜志;2005年09期

10 劉亞,蔡錦方,李振貴;重組人促紅細(xì)胞生成素對(duì)骨骼肌缺血再灌注損傷的保護(hù)作用[J];中國矯形外科雜志;2005年21期

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