本文選題：RNA + 干擾�。� 參考：《重慶醫(yī)科大學(xué)》2005年博士論文

【摘要】：膿毒癥(sepsis)至今仍是臨床治療中的難點(diǎn),其導(dǎo)致的死亡率高達(dá) 30-40%,而在所有膿毒癥病例中,由革蘭氏陰性(G-)細(xì)菌感染引起者約占 60%。內(nèi)毒素又名脂多糖(lipopolysaccharide, LPS),是 G-細(xì)菌細(xì)胞壁上的主要成分,也是 G-細(xì)菌最為重要的致病物質(zhì),它能激活單核-巨噬細(xì)胞系統(tǒng)(mononuclear phagocytic system, MPS),引起TNF-α 等大量前炎癥介質(zhì)釋放,導(dǎo)致全身炎癥反應(yīng)綜合征(systemic inflammatory response syndrome, SIRS)及器官損害。 然而,機(jī)體在遭受LPS刺激的同時(shí)也能產(chǎn)生相應(yīng)的保護(hù)機(jī)制——即內(nèi)毒素耐受(endotoxin tolerance)狀態(tài)。所謂內(nèi)毒素耐受是指:機(jī)體或單核-巨噬細(xì)胞經(jīng)LPS預(yù)處理后,對(duì)LPS的再次刺激呈低反應(yīng)性。目前認(rèn)為,內(nèi)毒素耐受是生物在長(zhǎng)期進(jìn)化過程中形成的一種保守的負(fù)反饋調(diào)節(jié)機(jī)制,是一種適應(yīng)性應(yīng)答,能避免了機(jī)體對(duì)LPS刺激的持續(xù)過度反應(yīng),是機(jī)體防御機(jī)制的重要組成部分。 最近的研究表明,白介素1受體相關(guān)激酶-M(IRAK-M)對(duì)LPS信號(hào)通路起負(fù)性調(diào)節(jié)作用,從而有可能參與內(nèi)毒素耐受的形成機(jī)制。本研究通過建立內(nèi)毒素耐受的小鼠及Kupffer細(xì)胞模型,觀察在內(nèi)毒毒耐受狀態(tài)下機(jī)體及細(xì)胞中IRAK-M表達(dá)的變化,初步確定IRAK-M與內(nèi)毒素耐受的關(guān)系;進(jìn)而采用RNA干擾技術(shù)在體外沉寂IRAK-M的基因表
[Abstract]:Sepsis (sepsis) is still a difficult point in clinical treatment, and the mortality rate is as high as 30-40%. In all cases of sepsis, about 60 cases are caused by Gram-negative G-negative bacterial infection. Lipopolysaccharide, also known as lipopolysaccharide, is the main component in the cell wall of G- bacteria and the most important pathogenic substance of G- bacteria. It can activate mononuclear phagocytic system (MPSN) of mononuclear macrophage system and induce the release of a large number of proinflammatory mediators, such as TNF- 偽. Lead to systemic inflammatory response syndrome, systemic inflammatory response syndrome, SIRS) and organ damage. However, LPS stimulates the body to produce the protective mechanism of endotoxin tolerance. Endotoxin tolerance means that the body or mononuclear macrophages pretreated with LPS are hyporeactive to the restimulation of LPS. It is believed that endotoxin tolerance is a kind of conservative negative feedback regulation mechanism formed in the long evolution of organism, and it is an adaptive response, which can avoid the persistent overreaction of organism to LPS stimulation. It is an important part of the body defense mechanism. Recent studies have shown that interleukin-1 receptor-associated kinases (IL-1 receptor-associated kinases) -MRAK-M1 play a negative role in the LPS signaling pathway, which may be involved in the formation of endotoxin tolerance. In order to determine the relationship between endotoxin tolerance and endotoxin tolerance, the changes of IRAK-M expression in body and cells were observed by establishing endotoxin tolerant mice and Kupffer cell models. Then RNA interference technique was used to silence the gene surface of IRAK-M in vitro.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2005
【分類號(hào)】：R363

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

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10 葉珩;鄧宇s，

本文編號(hào)：1952850