本文選題：乙肝病毒X蛋白 + β-連環(huán)蛋白　； 參考：《重慶醫(yī)科大學(xué)》2006年碩士論文

【摘要】： 乙型肝炎病毒(hepatitis B virus,HBV)編碼的X蛋白在HBV致癌過程中發(fā)揮著關(guān)鍵性的作用。HBX作為反式作用因子,不能直接和雙鏈DNA結(jié)合,而是通過蛋白與蛋白間的相互作用行使功能,可以調(diào)節(jié)轉(zhuǎn)錄、引起DAN修復(fù)蛋白質(zhì)失活以及激活信號轉(zhuǎn)導(dǎo)通路。Wnt信號傳導(dǎo)途徑在胚胎發(fā)育和腫瘤發(fā)生過程中起著十分重要的作用,而β-連環(huán)蛋白(β-catenin)是這個(gè)信號傳導(dǎo)途徑中的一個(gè)重要成員。HBX和β-catenin關(guān)系的研究對HBV導(dǎo)致肝癌的發(fā)生機(jī)制、肝癌的預(yù)防和治療都有重要意義,為闡明Wnt信號轉(zhuǎn)導(dǎo)途徑中β-catenin在肝癌發(fā)生和發(fā)展中的作用提供理論依據(jù)。 本課題采用帶有GFP的腺病毒高表達(dá)載體系統(tǒng)有效轉(zhuǎn)染人正常肝細(xì)胞系L02,并用Western blot證實(shí)HBX是否能有效在L02細(xì)胞中表達(dá)。用RT-PCR、免疫細(xì)胞化學(xué)方法分析HBx對正常細(xì)胞中β-catenin定位及基因表達(dá)的變化,并通過免疫共沉淀的方法來分析HBX與β-catenin、HBX與APC蛋白之間是否存在直接相互作用,了解HBX對正常肝細(xì)胞中β-catenin的影響及作用機(jī)制,為進(jìn)一步研究HBX導(dǎo)致肝癌的機(jī)制及其預(yù)防治療奠定基礎(chǔ)。
[Abstract]:The X protein encoded by hepatitis B virus (HBV) plays a key role in the carcinogenesis of HBV. As a trans-acting factor, HBX does not bind directly to double-stranded DNA, but functions through the interaction between protein and protein. It can regulate transcription, cause DAN repair protein inactivation and activate signal transduction pathway. Wnt signal transduction pathway plays an important role in embryonic development and tumorigenesis. 尾 -catenin is an important member of this signaling pathway. The study of the relationship between HBX and 尾 -catenin plays an important role in the pathogenesis, prevention and treatment of hepatocellular carcinoma induced by HBV. In order to elucidate the role of 尾 -catenin in the pathogenesis and development of hepatocellular carcinoma in Wnt signal transduction pathway. In this study, adenovirus high expression vector system with GFP was used to transfect human normal liver cell line L02, and Western blot was used to confirm whether HBX could be expressed effectively in L02 cells. The changes of 尾 -catenin localization and gene expression in normal cells were analyzed by RT-PCR and immunocytochemistry, and the direct interaction between HBX and 尾 -catenin HBX and APC protein was analyzed by immunoprecipitation. To understand the effect and mechanism of HBX on 尾 -catenin in normal hepatocytes, and to lay a foundation for further study on the mechanism of HBX induced liver cancer and its prevention and treatment.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R373

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本文編號：1947635