本文選題：苯甲酸雌二醇 + 己酸羥孕酮��； 參考：《天津醫(yī)科大學(xué)》2005年碩士論文

【摘要】：目的 觀察苯甲酸雌二醇、己酸羥孕酮以及二者聯(lián)用替代療法對(duì)卵巢切除小鼠免疫器官內(nèi)淋巴細(xì)胞數(shù)量、形態(tài)的變化,以及外周血白細(xì)胞、淋巴細(xì)胞數(shù)量變化,從而探討苯甲酸雌二醇、己酸孕酮對(duì)機(jī)體免疫系統(tǒng)的影響。 方法 采用健康雌性昆明小鼠,35天時(shí)隨機(jī)選取48只去勢(shì)后分成四組,分別注射生理劑量的苯甲酸雌二醇(OVX+EB組)、己酸羥孕酮(OVX+HPC組)或苯甲酸雌二醇、己酸羥孕酮聯(lián)用(OVX+EB.HPC組)以及麻油(麻油對(duì)照組,OVX)。另設(shè)一組為保留卵巢空白對(duì)照組(INT)。藥物注射從小鼠40天開始,每5天注射藥物一次,注射30次,共150天。光鏡下觀察免疫器官內(nèi)T、B淋巴細(xì)胞居住區(qū)范圍大小的變化;應(yīng)用MIAS—2000系統(tǒng)分別測(cè)定胸腺、脾、淋巴結(jié)T、B淋巴細(xì)胞居住區(qū)細(xì)胞密度;電鏡下觀察各組淋巴結(jié)內(nèi)T、B細(xì)胞超微結(jié)構(gòu)的改變。油鏡下進(jìn)行外周血白細(xì)胞、淋巴細(xì)胞計(jì)數(shù)。 結(jié)果 五組之間進(jìn)行比較,雌、孕激素對(duì)胸腺皮質(zhì)細(xì)胞數(shù)量均無影響(P0.05)。OVX+EB和OVX+EB.HPC組與INT組比較均可降低胸腺髓質(zhì)細(xì)胞密度,但二者之間差異在統(tǒng)計(jì)學(xué)上無意義。OVX+HPC組髓質(zhì)成熟T細(xì)胞密度最高。OVX組髓質(zhì)細(xì)胞密度高于INT。光鏡下各組胸腺皮質(zhì)細(xì)胞區(qū)域可見OVX+EB和OVX+EB.HPC皮質(zhì)區(qū)域擴(kuò)大,其余三組不能分辨有何區(qū)別。光鏡下觀察髓質(zhì)可見,與INT組相比,OVX+EB組髓質(zhì)區(qū)域明顯縮小,細(xì)胞稀疏,胸腺脂肪化程度最高;OVX+HPC組髓質(zhì)區(qū)域擴(kuò)大,細(xì)胞密集;OVX+EB.HPC組髓質(zhì)區(qū)域大小較INT組無明顯改變:OVX組髓質(zhì)區(qū)域明顯擴(kuò)大,脂肪化程度最低。 比較各組脾白髓中央動(dòng)脈周圍淋巴鞘內(nèi)的T細(xì)胞密度和脾小體內(nèi)B細(xì)胞密度出現(xiàn)以下結(jié)果:OVX+EB組與INT組相比T細(xì)胞密度無差異。OVX+EB.HPC
[Abstract]:Objective to observe the changes of lymphocyte number and morphology in the immune organs of ovariectomized mice by estradiol benzoate, hydroxyprogesterone caproate and their combination therapy, as well as the changes of the number of white blood cells and lymphocytes in peripheral blood of ovariectomized mice. The effects of estradiol benzoate and progesterone hexanoate on immune system were studied. Methods A total of 48 healthy female Kunming mice were randomly divided into four groups after castration for 35 days. Each group was given physiological dose of estradiol benzoate (OVX EB group, OVX HPC group) or estradiol benzoate (Estradiol benzoate). Hydroxyprogesterone caproate combined with OVX EB.HPC group and sesame oil (control group). Another group was used as control group. Drug injection began in mice for 40 days and was injected once every 5 days, 30 times for 150 days. The cell density of thymus, spleen and lymph node were measured by MIAS-2000 system in the living area of T _ (B) B lymphocytes in the immune organs under the light microscope, and the density of the cells in the living areas of the thymus, spleen and lymph nodes were measured by MIAS-2000 system. The ultrastructural changes of TnB cells in lymph nodes were observed under electron microscope. The peripheral blood leukocytes and lymphocytes were counted under oil microscope. Results there was no effect of estrogen and progesterone on the number of thymic cortical cells in the five groups. The density of thymic medulla cells in OVX EB.HPC group and INT group was significantly lower than that in INT group. But the difference between the two groups was statistically insignificant. The density of medullary mature T cells in OVX HPC group was the highest. The medullary cell density in OVX group was higher than that in INT group. Under the light microscope, the OVX EB and OVX EB.HPC cortical regions were enlarged in the thymic cortex of each group, but the other three groups could not distinguish the difference. Compared with the INT group, the medullary area in the OVX EB group was obviously reduced, the cells were sparse, the thymic fat degree was the highest and the medulla area was enlarged in the OVX HPC group. Compared with INT group, the medullary area of OVX EB.HPC group was significantly larger than that of INT group, and the fatty degree was the lowest in OVX group. The density of T cells in peri-lymphatic sheath of central splenic pulposus and B cell density in splenic small body were compared. The results showed that there was no difference in T cell density between the two groups. OVX EB.HPC was not different between the EB group and the INT group.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R392

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