licD2基因?qū)Ψ窝祖溓蚓玖τ绊懷芯?/H1>
發(fā)布時間：2018-05-21 02:20
本文選題：肺炎鏈球菌 + 毒力基因��； 參考：《重慶醫(yī)科大學(xué)》2005年碩士論文
【摘要】：肺炎鏈球菌(Streptococcus pneumoniae,S.pn)是全世界范圍內(nèi)引起感染和死亡的主要病原菌之一,是引起社區(qū)獲得性肺炎、中耳炎和腦膜炎最常見的病原菌。一百多年來,科學(xué)家們不斷探尋肺炎鏈球菌致病的機理,雖然揭示出許多肺炎鏈球菌感染的基本理論,然而到目前為止,人類對肺炎鏈球菌引發(fā)的疾病仍缺乏有效的預(yù)防手段。要徹底解決肺炎鏈球菌感染的問題,仍需深入探討肺炎鏈球菌致病的分子機理。 膽堿不僅是肺炎鏈球菌生長所需因子,在細胞分裂、轉(zhuǎn)化和自溶等生物學(xué)特性中發(fā)揮作用,還是影響多種肺炎鏈球菌毒力因子的重要物質(zhì),在肺炎鏈球菌生理代謝和發(fā)病機制中發(fā)揮著重要作用。而 licD2基因是掌控膽堿代謝的重要遺傳學(xué)位點,因此該基因?qū)Ψ窝祖溓蚓玖哂兄卮笥绊憽?目的:構(gòu)建 licD2 基因缺陷的肺炎鏈球菌菌株,探索該基因的缺陷是否影響細菌毒力。方法:采用基因克隆技術(shù),在肺炎鏈球菌自殺質(zhì)粒 pEVP3 中插入 licD2 基因片段。利用基因同源重組的原理,將重組自殺質(zhì)粒整合到肺炎鏈球菌染色體中建立穩(wěn)定的重組菌株。通過相對定量 RT-PCR 分析肺炎鏈球菌多種毒力因子表達情況,并利用動物體內(nèi)試驗觀察 licD2 基因缺陷菌株毒力改變。結(jié)果:①構(gòu)建了 pEVP3-licD2質(zhì)粒,成功建立了 licD2 基因缺陷的肺炎鏈球菌菌株。②licD2 缺陷以后,肺炎鏈球菌轉(zhuǎn)化能力明顯下降甚至完全不能轉(zhuǎn)化。③從體外培養(yǎng)細菌的毒力基因 mRNA 表達來看,缺陷型肺炎鏈球菌幾種毒力因子的
[Abstract]:Streptococcus pneumoniae (S.pn) is one of the major pathogens causing infection and death worldwide. It is the most common pathogen causing community acquired pneumonia, otitis media and meningitis. In the past more than 100 years, scientists have been exploring the pathogenesis of Streptococcus pneumoniae, although it reveals a lot of Streptococcus pneumoniae. The basic theory of dyeing, however, is still lack of effective preventive measures for Streptococcus pneumoniae caused by Streptococcus pneumoniae. To solve the problem of Streptococcus pneumoniae infection, the molecular mechanism of Streptococcus pneumoniae still needs to be explored.
Choline is not only a necessary factor for the growth of Streptococcus pneumoniae, but also plays an important role in biological characteristics such as cell division, transformation and autolysis. It plays an important role in the physiological metabolism and pathogenesis of Streptococcus pneumoniae. The licD2 gene is an important genetics in control of choline metabolism. Therefore, the gene has a significant effect on the virulence of Streptococcus pneumoniae.
Aim: to construct licD2 gene defective Streptococcus pneumoniae and explore whether the gene's defects affect the bacterial virulence. Methods: gene cloning technique was used to insert licD2 gene fragment into the pEVP3 of Streptococcus pneumoniae suicide plasmid. The recombinant self killing plasmid was integrated into the chromosome of Streptococcus pneumoniae by the principle of homologous recombination. A relatively quantitative RT-PCR was used to analyze the expression of various virulence factors of Streptococcus pneumoniae and to observe the change of virulence of licD2 gene defective strains in vivo. Results: (1) a pEVP3-licD2 plasmid was constructed and a Streptococcus pneumoniae with licD2 gene defect was successfully established. (2) after the licD2 defect, pneumonia The ability to convert Streptococcus mutans to a significant decrease or even completely cannot be converted. (3) several virulence factors of Streptococcus pneumoniae from the expression of the virulence gene mRNA of the bacteria in vitro
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R378
【參考文獻】
相關(guān)期刊論文 前1條
1 王[?,尹一兵,豬川嗣朗,羅進勇,張雪梅;肺炎鏈球菌感受態(tài)的形成影響毒力因子mRNA的表達[J];中華微生物學(xué)和免疫學(xué)雜志;2003年03期
，
本文編號：1917303
本文鏈接：http://sikaile.net/yixuelunwen/binglixuelunwen/1917303.html
本文選題：肺炎鏈球菌 + 毒力基因��； 參考：《重慶醫(yī)科大學(xué)》2005年碩士論文
【摘要】：肺炎鏈球菌(Streptococcus pneumoniae,S.pn)是全世界范圍內(nèi)引起感染和死亡的主要病原菌之一,是引起社區(qū)獲得性肺炎、中耳炎和腦膜炎最常見的病原菌。一百多年來,科學(xué)家們不斷探尋肺炎鏈球菌致病的機理,雖然揭示出許多肺炎鏈球菌感染的基本理論,然而到目前為止,人類對肺炎鏈球菌引發(fā)的疾病仍缺乏有效的預(yù)防手段。要徹底解決肺炎鏈球菌感染的問題,仍需深入探討肺炎鏈球菌致病的分子機理。 膽堿不僅是肺炎鏈球菌生長所需因子,在細胞分裂、轉(zhuǎn)化和自溶等生物學(xué)特性中發(fā)揮作用,還是影響多種肺炎鏈球菌毒力因子的重要物質(zhì),在肺炎鏈球菌生理代謝和發(fā)病機制中發(fā)揮著重要作用。而 licD2基因是掌控膽堿代謝的重要遺傳學(xué)位點,因此該基因?qū)Ψ窝祖溓蚓玖哂兄卮笥绊憽?目的:構(gòu)建 licD2 基因缺陷的肺炎鏈球菌菌株,探索該基因的缺陷是否影響細菌毒力。方法:采用基因克隆技術(shù),在肺炎鏈球菌自殺質(zhì)粒 pEVP3 中插入 licD2 基因片段。利用基因同源重組的原理,將重組自殺質(zhì)粒整合到肺炎鏈球菌染色體中建立穩(wěn)定的重組菌株。通過相對定量 RT-PCR 分析肺炎鏈球菌多種毒力因子表達情況,并利用動物體內(nèi)試驗觀察 licD2 基因缺陷菌株毒力改變。結(jié)果:①構(gòu)建了 pEVP3-licD2質(zhì)粒,成功建立了 licD2 基因缺陷的肺炎鏈球菌菌株。②licD2 缺陷以后,肺炎鏈球菌轉(zhuǎn)化能力明顯下降甚至完全不能轉(zhuǎn)化。③從體外培養(yǎng)細菌的毒力基因 mRNA 表達來看,缺陷型肺炎鏈球菌幾種毒力因子的
[Abstract]:Streptococcus pneumoniae (S.pn) is one of the major pathogens causing infection and death worldwide. It is the most common pathogen causing community acquired pneumonia, otitis media and meningitis. In the past more than 100 years, scientists have been exploring the pathogenesis of Streptococcus pneumoniae, although it reveals a lot of Streptococcus pneumoniae. The basic theory of dyeing, however, is still lack of effective preventive measures for Streptococcus pneumoniae caused by Streptococcus pneumoniae. To solve the problem of Streptococcus pneumoniae infection, the molecular mechanism of Streptococcus pneumoniae still needs to be explored.
Choline is not only a necessary factor for the growth of Streptococcus pneumoniae, but also plays an important role in biological characteristics such as cell division, transformation and autolysis. It plays an important role in the physiological metabolism and pathogenesis of Streptococcus pneumoniae. The licD2 gene is an important genetics in control of choline metabolism. Therefore, the gene has a significant effect on the virulence of Streptococcus pneumoniae.
Aim: to construct licD2 gene defective Streptococcus pneumoniae and explore whether the gene's defects affect the bacterial virulence. Methods: gene cloning technique was used to insert licD2 gene fragment into the pEVP3 of Streptococcus pneumoniae suicide plasmid. The recombinant self killing plasmid was integrated into the chromosome of Streptococcus pneumoniae by the principle of homologous recombination. A relatively quantitative RT-PCR was used to analyze the expression of various virulence factors of Streptococcus pneumoniae and to observe the change of virulence of licD2 gene defective strains in vivo. Results: (1) a pEVP3-licD2 plasmid was constructed and a Streptococcus pneumoniae with licD2 gene defect was successfully established. (2) after the licD2 defect, pneumonia The ability to convert Streptococcus mutans to a significant decrease or even completely cannot be converted. (3) several virulence factors of Streptococcus pneumoniae from the expression of the virulence gene mRNA of the bacteria in vitro
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R378
【參考文獻】
相關(guān)期刊論文 前1條
1 王[?,尹一兵,豬川嗣朗,羅進勇,張雪梅;肺炎鏈球菌感受態(tài)的形成影響毒力因子mRNA的表達[J];中華微生物學(xué)和免疫學(xué)雜志;2003年03期
，本文編號：1917303
本文鏈接：http://sikaile.net/yixuelunwen/binglixuelunwen/1917303.html
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