本文選題：氟苯尼考 + 藥物殘留　； 參考：《西南民族大學(xué)》2006年碩士論文

【摘要】： 為研究氟苯尼考?xì)埩魧?duì)細(xì)菌耐藥性的影響,本文采用含氟苯尼考的培養(yǎng)基模擬機(jī)體腸道內(nèi)藥物殘留環(huán)境,進(jìn)行了兩部分試驗(yàn): 試驗(yàn)一是在體外誘導(dǎo)三種人體腸道細(xì)菌——大腸桿菌、產(chǎn)氣夾膜梭菌、糞腸球菌分別對(duì)氟苯尼考產(chǎn)生不同水平的耐菌性,然后采用二倍稀釋法檢測(cè)這些耐藥菌株對(duì)氯霉素、頭孢曲松、氨芐西林、四環(huán)素、土霉素、慶大霉素、阿米卡星、氧氟沙星、環(huán)丙沙星等常規(guī)藥物敏感性變化。結(jié)果顯示,對(duì)于不同的細(xì)菌,氟苯尼考與上述不同種藥物存在不同程度的交叉耐藥性。 試驗(yàn)二是在體外誘導(dǎo)動(dòng)物源性大腸桿菌O_(78)對(duì)氟苯尼考產(chǎn)生不同水平的耐藥性,觀察不同誘導(dǎo)時(shí)間和不同的藥物濃度對(duì)耐藥性性的影響。結(jié)果顯示,對(duì)于同一誘導(dǎo)藥物濃度,誘導(dǎo)時(shí)間越長(zhǎng),細(xì)菌耐藥水平越高,但增長(zhǎng)速度減慢,最后趨于一穩(wěn)定水平。當(dāng)誘導(dǎo)藥物濃度為2μg/ml、64μg/ml、128μg/ml時(shí),細(xì)菌耐藥性增長(zhǎng)速度慢,當(dāng)誘導(dǎo)藥物濃度為4μg/ml、8μg/ml、16μg/ml、32μg/ml時(shí),細(xì)菌耐藥性增長(zhǎng)速度快。 通過(guò)本試驗(yàn)結(jié)果,證實(shí)動(dòng)物專用抗菌藥物殘留對(duì)人體存在一定影響,并影響人類臨床用藥。同時(shí),還比較客觀的觀察了氟苯尼考耐藥菌株的產(chǎn)生過(guò)程,比較了不同殘留水平和殘留時(shí)間對(duì)耐藥性的產(chǎn)生的影響。這些結(jié)論,對(duì)于臨床合理使用氟苯尼考,減少殘留,延緩細(xì)菌耐藥性增長(zhǎng)速度,延長(zhǎng)氟苯尼考臨床使用壽命,具有一定參考價(jià)值。
[Abstract]:In order to study the effect of florfenicol residue on bacterial drug resistance, a two-part experiment was carried out in this paper, in which florfenicol containing florfenicol was used to simulate the environment of drug residues in intestinal tract. The first experiment was to induce in vitro three kinds of human intestinal bacteria, E. coli, Clostridium aerogenes and Enterococcus faecalis, to produce different levels of resistance to florfenicol, and then double dilution method was used to detect the resistance of these resistant strains to chloramphenicol. Changes in sensitivity of ceftriaxone, ampicillin, tetracycline, oxytetracycline, gentamicin, amikacin, ofloxacin and ciprofloxacin. The results showed that florfenicol had different cross-resistance to different bacteria. The second experiment was to induce different levels of drug resistance to florfenicol in vitro. The effects of different induction time and drug concentration on drug resistance were observed. The results showed that for the same drug concentration, the longer the induction time was, the higher the drug resistance level of bacteria was, but the growth rate slowed down and finally tended to a stable level. When the concentration of induced drug was 2 渭 g / ml, 64 渭 g / ml, 128 渭 g/ml, the drug resistance of bacteria increased slowly. When the concentration of induced drug was 4 渭 g / ml, 16 渭 g / ml or 32 渭 g/ml, the drug resistance of bacteria increased rapidly. The results show that animal-specific antimicrobial residues have a certain impact on human body and affect human clinical drug use. At the same time, the production process of florfenicol resistant strains was observed objectively, and the effects of different residue levels and residual time on drug resistance were compared. These conclusions have certain reference value for the rational use of florfenicol in clinic, reducing residues, delaying the growth rate of bacterial resistance and prolonging the clinical service life of florfenicol.
【學(xué)位授予單位】：西南民族大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R155.5;R378

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前1條

1 舒文婷;亞胺培南對(duì)銅綠假單胞菌DNA釋放和形態(tài)的影響[D];遵義醫(yī)學(xué)院;2013年

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本文編號(hào)：1916052