本文選題：小鼠 + 腹側(cè)蓋膜區(qū)　； 參考：《大連醫(yī)科大學(xué)》2005年碩士論文

【摘要】：腹側(cè)蓋膜區(qū)(ventral tegmental area,VTA)的多巴胺細胞與回報行為以及認知功能有著密切的關(guān)系。多巴胺細胞有兩種放電模式,規(guī)律放電和 bursting。Bursting 這種放電模式與回報行為有關(guān),而在細胞學(xué)上其可以增加突觸內(nèi)神經(jīng)遞質(zhì)多巴胺的含量。在大鼠的研究中,apamin 和 NMDA 都可以在多巴胺細胞上誘發(fā) bursting,但其作用機理不同,apamin 誘發(fā)的 bursting 可以被 nifedipine 抑制,而相反 NMDA誘發(fā)的 bursing 不能被 nifedipine 抑制。這提示在生物系統(tǒng)中,bursting這種放電模式可以通過不同的兩套系統(tǒng)實現(xiàn)。但是 apamin 誘發(fā)的bursting 是依賴哪種神經(jīng)傳導(dǎo)系統(tǒng)尚沒有定論。而目前的研究大多數(shù)集中在大鼠,對小鼠放電行為的研究很少。故本課題著重探討了小鼠VTA 區(qū)多巴胺細胞放電的行為模式,同時試圖探討 NMDA 和carbachol 誘發(fā)的 bursting 的不同機理。 1. 體外小鼠 VTA 區(qū)多巴胺細胞主要存在兩種放電模式。 研究結(jié)果指出:在室溫 perforated 膜片鉗技術(shù)下,小鼠 VTA 區(qū)多巴胺細胞 31%靜止不自發(fā)放電,65%呈現(xiàn)規(guī)律或者不規(guī)律的放電模式,4%呈現(xiàn)自發(fā) bursting 的放電模式。 2. NMDA 誘發(fā)的 bursting 不是由鈣離子介導(dǎo)的。 研究結(jié)果指出:NMDA 可以在 32%記錄的小鼠 VTA 區(qū)的多巴胺細胞上誘發(fā)出 bursting,其不能被電壓依賴性鈣離子通道的抑制劑抑制(n=5);同時用 TTX 抑制 sodium spikes 后呈現(xiàn)出的膜電位震蕩同樣不能被電壓依賴性鈣離子通道的抑制劑抑制(n=3)。
[Abstract]:The dopamine cells of ventral tegmental are closely related to reward behavior and cognitive function. There are two discharge modes in dopamine cells: regular discharge and bursting.Bursting, which are related to the behavior of reward, but it can increase the content of dopamine in synaptic neurotransmitter in cytology. In the study of rats, both apamin and NMDA could induce burstling in dopamine cells, but bursting induced by apamin in different mechanisms could be inhibited by nifedipine, but bursing induced by NMDA could not be inhibited by nifedipine. This suggests that in biological systems bursting can be implemented in two different systems. But it is not clear what kind of nerve conduction system is dependent on bursting induced by apamin. However, most of the current studies are focused on rats, and little research has been done on the discharges in mice. Therefore, this study focused on the behavioral pattern of dopamine cell discharges in VTA region of mice, and tried to explore the different mechanisms of bursting induced by NMDA and carbachol. 1. There are two kinds of discharge patterns in dopamine cells in VTA region of mice in vitro. The results showed that at room temperature perforated patch clamp technique, 31% of the dopamine cells in the VTA region of mice showed regular or irregular discharge patterns (65%), or 4% (4%) of the dopamine cells in the VTA region showed spontaneous bursting discharge patterns. 2. Bursting induced by NMDA is not mediated by calcium ion. The results showed that the burstingwas induced in 32% of the mouse VTA dopamine cells, which could not be inhibited by the voltage-dependent calcium channel inhibitor, and the membrane electricity after sodium spikes was inhibited by TTX. Position oscillation also cannot be inhibited by voltage-dependent calcium channel inhibitors.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R33

【共引文獻】

相關(guān)碩士學(xué)位論文 前2條

1 王姍姍;谷氨酸能突觸對腹側(cè)被蓋區(qū)多巴胺能神經(jīng)元興奮性的影響[D];陜西師范大學(xué);2011年

2 劉國鵬;黑質(zhì)致密部神經(jīng)元的“非周期敏感”現(xiàn)象[D];第四軍醫(yī)大學(xué);2001年

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本文編號：1901849