本文選題：網(wǎng)狀結(jié)構(gòu) + 巨細(xì)胞網(wǎng)狀核��； 參考：《鄭州大學(xué)》2007年碩士論文

【摘要】： 背景和目的 腦干網(wǎng)狀結(jié)構(gòu)是中樞神經(jīng)內(nèi)的一個(gè)整合中心，從此中心不斷發(fā)放沖動(dòng)傳導(dǎo)信息到大腦皮質(zhì)、脊髓和小腦等其它腦區(qū)發(fā)揮調(diào)節(jié)作用。傳統(tǒng)上網(wǎng)狀結(jié)構(gòu)被視作中樞神經(jīng)內(nèi)一個(gè)古老的、彌散的結(jié)構(gòu)。其內(nèi)除少數(shù)邊界較清晰的核群外，似乎不易再劃分其他核群：在功能上除參與內(nèi)臟活動(dòng)的調(diào)節(jié)(如呼吸、循環(huán))外，看不出有什么重要作用。但進(jìn)一步研究則提示腦干網(wǎng)狀結(jié)構(gòu)可能有許多更為重要和復(fù)雜的功能。關(guān)于它的結(jié)構(gòu)，后來知道也不似原來想象的那么簡(jiǎn)單。隨著熒光組織化學(xué)和免疫細(xì)胞化學(xué)方法的應(yīng)用，許多特異神經(jīng)遞質(zhì)的神經(jīng)元在網(wǎng)狀結(jié)構(gòu)內(nèi)被發(fā)現(xiàn)。但至今，對(duì)于網(wǎng)狀結(jié)構(gòu)還有許多問題尚待解決。 中樞神經(jīng)系統(tǒng)發(fā)揮功能的基礎(chǔ)來源于其形態(tài)學(xué)結(jié)構(gòu)。網(wǎng)狀結(jié)構(gòu)內(nèi)其傳出纖維主要投射到4個(gè)區(qū)域：丘腦、脊髓、小腦和腦干的其他結(jié)構(gòu)。其作用于脊髓的纖維束對(duì)骨骼肌興奮和抑制的調(diào)節(jié)有重要作用，從而調(diào)控了人肢體運(yùn)動(dòng)的隨意和精確性。在網(wǎng)狀結(jié)構(gòu)向脊髓傳出纖維進(jìn)行調(diào)控的同時(shí)，脊髓的神經(jīng)元亦投射纖維到網(wǎng)狀結(jié)構(gòu)內(nèi)，參與了網(wǎng)狀結(jié)構(gòu)對(duì)運(yùn)動(dòng)神經(jīng)元調(diào)控的反饋和控制。 為進(jìn)一步了解網(wǎng)狀結(jié)構(gòu)和脊髓神經(jīng)元之間的纖維聯(lián)系，為網(wǎng)狀結(jié)構(gòu)對(duì)運(yùn)動(dòng)的調(diào)控提供更多的理論依據(jù)，我們選取了網(wǎng)狀結(jié)構(gòu)內(nèi)與運(yùn)動(dòng)調(diào)控密切相關(guān)的伸肌抑制區(qū)內(nèi)的重要核團(tuán)—巨細(xì)胞網(wǎng)狀核(gigantocellular reticular nucleus，NGC)進(jìn)行研究，探求它和頸段脊髓的纖維聯(lián)系。 方法 1、麥芽凝集素—辣根過氧化物酶的微量注射：選擇成年大鼠，在大鼠右側(cè)巨細(xì)胞網(wǎng)狀核內(nèi)微量注射麥芽凝集素—辣根過氧化物酶神經(jīng)示蹤劑。同時(shí)建立陰性對(duì)照組進(jìn)行對(duì)照比較。 2、麥芽凝集素—辣根過氧化物酶的檢測(cè)：注射48h后用TMB顯色方法檢測(cè)巨細(xì)胞網(wǎng)狀核和頸段脊髓灰質(zhì)內(nèi)麥芽凝集素—辣根過氧化物酶的表達(dá)情況，并進(jìn)行數(shù)據(jù)采集和分析。 3、海人酸的微量注射：同樣在大鼠右側(cè)巨細(xì)胞網(wǎng)狀核內(nèi)微量注射海人酸，造成對(duì)巨細(xì)胞網(wǎng)狀核內(nèi)神經(jīng)纖維的傷害性刺激。 4、c-fos基因表達(dá)情況的檢測(cè)：海人酸注入2h后檢測(cè)實(shí)驗(yàn)大鼠頸段脊髓灰質(zhì)內(nèi)c-fos基因表達(dá)情況，以此推斷巨細(xì)胞網(wǎng)狀核和脊髓的纖維聯(lián)系。 結(jié)果 1、在巨細(xì)胞網(wǎng)狀核內(nèi)注射麥芽凝集素—辣根過氧化物酶，可在頸段脊髓灰質(zhì)Ⅰ、Ⅱ板層發(fā)現(xiàn)順行標(biāo)記，在Ⅶ、Ⅷ板層發(fā)現(xiàn)逆行標(biāo)記。 2、在巨細(xì)胞網(wǎng)狀核內(nèi)注入傷害性刺激物海人酸可以引起頸段脊髓灰質(zhì)Ⅶ、Ⅷ板層內(nèi)c-fos基因的顯著性表達(dá)，與陰性對(duì)照組相比具有顯著性差別，以α=0.05為檢驗(yàn)水準(zhǔn)，，兩組之間的差別具有統(tǒng)計(jì)學(xué)意義。 結(jié)論 1、通過對(duì)WGA-HRP示蹤和c-fos基因表達(dá)情況的檢測(cè)，說明以巨細(xì)胞網(wǎng)狀核為主的腦干網(wǎng)狀結(jié)構(gòu)抑制區(qū)和頸段脊髓神經(jīng)元之間存在直接的纖維聯(lián)系。 2、從巨細(xì)胞網(wǎng)狀核到脊髓的投射纖維多經(jīng)由頸段脊髓灰質(zhì)的Ⅰ、Ⅱ板層。而頸段脊髓灰質(zhì)向巨細(xì)胞網(wǎng)狀核的投射纖維多起源于Ⅶ、Ⅷ板層。
[Abstract]:Background and purpose
The brain stem reticular formation is an integration center in the central nervous system. From then on, impulse transmission information is continuously distributed to the cerebral cortex, and other brain regions, such as the spinal cord and the cerebellum, play an regulatory role. The traditional Internet structure is regarded as an old, diffuse structure in the central nerve. It seems not easy to be within a few clear nuclei. Further studies suggest that the brain stem reticular structure may have many more important and complex functions. The application of studies and immunocytochemical methods, many specific neurotransmitter neurons are found in the reticular formation. But there are still many problems still to be solved for reticular formation.
The basis of the function of the central nervous system is derived from its morphological structure. In the reticular structure its efferent fibers are projected mainly into 4 regions: the thalamus, spinal cord, cerebellum, and other brain stem structures. The fiber bundles acting on the spinal cord play an important role in the regulation of excitability and inhibition of skeletal muscles, thereby regulating the random and sperm movement of the human body. It is true that the neurons of the spinal cord also project the fibers into the reticular formation while the reticular formation of the spinal cord is transmitted to the spinal cord, and participates in the feedback and control of the reticular formation of the motor neurons.
In order to further understand the fiber connections between the reticular and spinal neurons, and to provide more theoretical basis for the regulation of the reticular formation of motion, we have selected the gigantocellular reticular nucleus (NGC), an important nucleus in the extensor inhibition zone, which is closely related to the regulation of motion. Explore the fibrous connection with the cervical spinal cord.
Method
1, microinjection of malt agglutinin - horseradish peroxidase: selected adult rats, microinjection of malt lectin to horseradish peroxidase nerve tracer in rat right giant cell reticular nucleus, and set up a negative control group for comparison.
2, detection of malt lectin - horseradish peroxidase: after injection of 48h, the expression of malt agglutinin - horseradish peroxidase in the giant cell reticular nucleus and cervical spinal cord gray matter was detected by TMB colorimetric method, and the data were collected and analyzed.
3, microinjection of sea human acid: also microinjection of sea human acid in the right giant cell reticular nucleus in the right side of the rat, causing injurious stimulation to the nerve fibers in the giant cell reticular nucleus.
4, detection of c-fos gene expression: the expression of c-fos gene in the gray matter of the cervical spinal cord was detected by sea human acid injected into 2H to infer the fibrous connection between the giant cell reticular nucleus and the spinal cord.
Result
1, the injection of malt lectin - horseradish peroxidase in the giant cell reticular nucleus could be found in the cervical spinal gray matter I and II lamina, and the retrograde labeling was found in the VII and VIII lamina.
2, injecting a nociceptive stimulator into the nucleus of the giant cell reticular nucleus could cause a significant expression of the c-fos gene in the gray matter VII and the VIII lamina of the cervical spinal cord. Compared with the negative control group, there was a significant difference. The difference between the two groups was statistically significant.
conclusion
1, through the detection of WGA-HRP tracer and c-fos gene expression, it is indicated that there is a direct fibrous connection between the brain stem reticular inhibition zone and the cervical spinal cord neurons based on the giant cell reticular nucleus.
2, the projective fibers from the giant cell reticular nucleus to the spinal cord are mostly through the cervical spinal gray matter I, II lamina, and the projection fibers from the gray matter of the cervical spinal cord to the giant cell reticular nucleus originate from the VII and the VIII lamina.

【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：R329

【共引文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 付彥君;酮色林對(duì)大鼠動(dòng)脈壓力感受性反射功能的影響及其機(jī)制探討[D];沈陽藥科大學(xué);2006年

相關(guān)碩士學(xué)位論文 前1條

1 常成;大鼠選擇性脊神經(jīng)后根切斷術(shù)緩解痙攣型腦癱泌涎癥狀的實(shí)驗(yàn)研究[D];鄭州大學(xué);2005年

本文編號(hào)：1896144