本文選題：臍血 + 骨髓　； 參考：《南昌大學(xué)》2006年碩士論文

【摘要】：目的：了解臍血間充質(zhì)干細胞(UCB-MSCs)對植物血凝素(PHA)誘導(dǎo)淋巴細胞(LCs)增殖的影響并與骨髓間充質(zhì)干細胞(BM-MSCs)作比較；探討UCB-MSCs對PHA誘導(dǎo)的LCs增殖抑制實驗中的最佳細胞量效比；測定UCB-MSCs各亞組轉(zhuǎn)化生長因子(TGF-β1)水平及肝素對UCB-MSCs分泌TGF-β1的影響。 方法：對培養(yǎng)的UCB-MSCs和BM-MSCs進行形態(tài)、表型及功能方面的綜合鑒定；將不同細胞濃度的UCB-MSCs和BM-MSCs加入由PHA誘導(dǎo)的LCs轉(zhuǎn)化實驗體系中，MTT法測定各亞組對LCs的抑制率。用ELISA法測定上清液中的TGF-β1水平。 結(jié)果：(1)用密度梯度離心的方法從UCB和人BM中分離單個核細胞(MNC)，用貼壁法培養(yǎng)MSC，通過形態(tài)、表型及功能進行綜合鑒定。培養(yǎng)至第3代的MSC具有長梭形的典型形態(tài)改變，表面分子檢測顯示高表達CD166、CD29、CD54，低表達CD13、CD34、CD45。經(jīng)體外誘導(dǎo)可成為脂肪細胞。(2)MSCs：LCs為1：1、0.5：1、0.1：1、0.05：1、0.02：1、0.01：1時，UCB-MSCs組對PHA刺激的LCs增殖均有抑制作用，，抑制率分別為49.5±4.8、58.4±6.0、38.1±4.0、31.4±3.2、24.3±3.2、12.6±6.7；而BM-MSCs組抑制率隨BM-MSCs數(shù)量不同而異，分別為52.4±8.4、65.1±9.7、34.7-±4.5、13.0±6.4、10.7±12.6、-43.9±9.4。UCB-MSCs組及BM-MSCs組在MSCs：LCs為0.05：1、0.02：1、0.01：1的三個亞組間對LCs的抑制率存在顯著差異(P＜0.05)。UCB-MSCs組抑制率強于BM-MSCs組。(3)當(dāng)UCB-MSCs：LCs為0.75：1時抑制作用最強，與其他亞組比較有顯著差異(P＜0.05)。(4)UCB-MSCs各亞組上清液中TGF-β1水平高于BM-MSCs組，呈細胞劑量依賴性；UCB-MSCs組抑制率與TGF-β1水平相關(guān)性略小
[Abstract]:Objective: to investigate the effect of UCB-MSCs on the proliferation of lymphocytes in vitro induced by plant hemagglutinin (PHA) and compare it with that of BM-MSCs (BM-MSCs), and to explore the best dose-effectiveness ratio of UCB-MSCs in inhibiting the proliferation of LCs induced by PHA. The levels of TGF- 尾 1 in UCB-MSCs subgroups and the effect of heparin on TGF- 尾 1 secretion by UCB-MSCs were determined. Methods: the morphological phenotypic and functional characteristics of cultured UCB-MSCs and BM-MSCs were comprehensively identified and UCB-MSCs and BM-MSCs of different cell concentrations were added to the LCs transformation system induced by PHA to determine the inhibition rate of LCs in each subgroup. The level of TGF- 尾 1 in supernatant was determined by ELISA method. Results the mononuclear cells were isolated from UCB and human BM by density gradient centrifugation. The mononuclear cells were cultured by adherent method. The MSC cultured to the third generation had typical morphological changes of long spindle shape. Surface molecular analysis showed high expression of CD166T CD29 CD54 and low expression of CD13 CD34 + CD45. After induction in vitro, the MSCs: LCs were 1: 1: 0.5: 1: 0.1: 1: 1 0.05: 1. The inhibitory rates of UCB-MSCs on the proliferation of LCs stimulated by PHA were 49.5 鹵4.88.4 鹵6.038.1 鹵4.038.1 鹵4.038.1 鹵4.038.1 鹵3.21.The inhibitory rates of BM-MSCs group varied with the number of BM-MSCs. There were significant differences in the inhibition rate of LCs between the three subgroups with MSCs:LCs of 0.05: 1: 1: 0.02: 0.01: 1 (P < 0.05).UCB-MSCs) and BM-MSCs group (P < 3). The inhibition rate of LCs was significantly higher in 0.05).UCB-MSCs group than in BM-MSCs group (P < 0.05: 1) when UCB-MSCs:LCs was 0.75: 1. The inhibitory rate of LCs was significantly higher in the BM-MSCs group than in the BM-MSCs group (10.7 鹵12.6 鹵43.9 鹵10.7 鹵10.7 鹵12.6ng-43.9 鹵1.The MSCs:LCs was 0.05: 10.02: 1: 1, respectively, P < 0.05), but the inhibition rate in the 0.05).UCB-MSCs group was significantly higher than that in the BM-MSCs group. The level of TGF- 尾 1 in supernatant of each subgroup of 0.05).(4)UCB-MSCs was significantly higher than that of other subgroups, and the inhibition rate of UCB-MSCs was slightly correlated with the level of TGF- 尾 1 in a dose-dependent manner.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R329.2

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