本文選題：間質(zhì)細(xì)胞 + 再生　； 參考：《河北醫(yī)科大學(xué)》2006年碩士論文

【摘要】： 目的:應(yīng)用硝基藍(lán)四唑鹽(NBT)法、HE染色和磁分離均相酶聯(lián)免疫法等方法觀察睪丸間質(zhì)細(xì)胞(Leydig cells, LCs)被二甲磺基乙烷(ethane dimethane sulphonate,EDS)破壞后在甲狀腺機(jī)能亢進(jìn)和甲狀腺機(jī)能減退情況下的再生情況,探討甲狀腺激素對成熟大鼠LCs再生的影響。 方法:選用90日齡雄性SD大鼠54只,EDS一次性腹腔注射(85mg/kg)破壞LCs。將54只EDS處理后的大鼠隨機(jī)分為三組:甲狀腺機(jī)能亢進(jìn)組、甲狀腺機(jī)能減退組和對照組,每組18只。 甲狀腺機(jī)能亢進(jìn)組大鼠在注射EDS當(dāng)日起每天皮下注射T3(60μg/kg)一次,造成甲狀腺機(jī)能亢進(jìn)模型;甲狀腺機(jī)能減退組大鼠在注射EDS當(dāng)日起每天用50mg丙硫氧嘧啶水溶液灌胃一次,建立甲狀腺機(jī)能減退模型;對照組大鼠在注射EDS當(dāng)日起每天皮下注射含0.025 M NaOH的生理鹽水0.8 ml。 分別在注射EDS后的第7天、14天和21天麻醉大鼠、取材,進(jìn)行如下指標(biāo)的觀察:①LCs的再生情況:取睪丸-70℃速凍,Leica冰凍切片機(jī)切片,片厚15μm,NBT法顯示LCs內(nèi)的3β-HSD酶,判定LCs的再生情況。②LCs的功能:取睪丸組織體外培養(yǎng),加入黃體生成素(LH)后,采用磁酶免法檢測上清液中睪酮的含量;下腔靜脈取血,離心取血清,磁酶免法檢測血清中睪酮的含量。③LCs的形態(tài)和數(shù)
[Abstract]:Objective: to observe the hyperthyroidism and hypothyroidism of Leydig cells (LCs) in testicular interstitial cells after the destruction of Leydig cells (LCs) by dimethylsulfoethane dimethane sulphonate EDSs by means of HE staining and magnetic separation homogenous enzyme linked immunosorbent assay (Elisa) with nitro blue tetrazolium (NBT). In the case of regeneration, To investigate the effect of thyroid hormone on LCs regeneration in mature rats. Methods: 54 male SD rats aged 90 days were injected intraperitoneally with single dose of 85 mg / kg to destroy LCs. Fifty-four EDS treated rats were randomly divided into three groups: hyperthyroidism group, hypothyroidism group and control group with 18 rats in each group. The hyperthyroidism model was established in hyperthyroidism group by subcutaneous injection of T3N 60 渭 g / kg daily from the day of EDS injection, and the hypothyroidism group rats were treated with 50mg prothiouracil solution once a day from the day of EDS injection. The hypothyroidism model was established and the control rats were subcutaneously injected with normal saline containing 0.025 M NaOH (0.8 ml / day) from the day of EDS injection. Anesthetized rats were anesthetized on the 7th day and 21st day after EDS injection, and the regeneration of 1 LCs was observed as follows: the testis were taken from the frozen Leica slicer at 70 鈩，

本文編號：1893691