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  本文選題：致倦庫蚊 + Mtx1��； 參考：《中國科學(xué)院研究生院（武漢病毒研究所）》2005年碩士論文

【摘要】：Mtx1 毒素是球形芽孢桿菌(Bacillus sphaericus)在營養(yǎng)體生長階段產(chǎn)生的一種殺蚊毒素,其作用機(jī)制和殺蟲活性不同于殺蚊二元毒素,具有潛在的應(yīng)用價值。本研究主要從Mtx1 毒素的殺蟲譜、Mtx1 毒素對致倦庫蚊的后致死作用、誘導(dǎo)致倦庫蚊(Culex quinquefasciatus)產(chǎn)生抗性及抗性蚊蟲的抗性特性方面進(jìn)行了研究。 生物測定表明,Mtx1 毒素對受試的幾種鱗翅目昆蟲幾乎沒有毒性,但對白紋伊蚊表現(xiàn)出了中等程度的毒性,對敏感和二元毒素抗性致倦庫蚊表現(xiàn)出了高度的毒性。表達(dá)Mtx1 毒素的E-TH21 冷凍粉劑對敏感致倦庫蚊品系SLCq 和二元毒素抗性品系RLCq1/C3-41 和RLCq2/IAB59 的LC_(50)值分別為0.508、0.854 和0.675mg/L。結(jié)果證明,Mtx1 毒素在控制或減緩致倦庫蚊對二元毒素的抗性方面具有潛在的應(yīng)用價值。 Mtx1 不僅對目標(biāo)蚊幼蟲具有特異性的毒殺作用,同時也具很強(qiáng)的后致死作用。分別以亞致死濃度(LC_(10)、LC_(30)、LC_(50)、LC_(70)、LC_(90)和LC_(98))的Mtx1 毒素和二元毒素制劑處理2-3 齡致倦庫蚊幼蟲48 小時后,經(jīng)處理的存活幼蟲在各生長階段都表現(xiàn)出了明顯的延續(xù)死亡和損傷現(xiàn)象,羽化前死亡率分別為85.0%、97.5%、98.6%、99.5%、100%、100%和23.1%、44.4%、61.6%、83.2%、95.1%、100%。Mtx1 毒素較二元毒素對致倦庫蚊有更強(qiáng)的后致死作用。在致倦庫蚊對Mtx1 毒素的抗性研究中,我們用高劑量的Mtx1 毒素制劑對致倦庫蚊進(jìn)行了30 代的篩選,得到了抗Mtx1 毒素的低水平抗性品系RLCq4/Mtx1,同敏感蚊蟲比較,其抗性水平約為223.2 倍。該品系對蘇云金芽孢桿菌以色列亞種(B. thuringiensis subsp. israelensis)和球形芽孢桿菌LP1-G 和47-6B 不產(chǎn)生交互抗性,對IAB59 菌株表現(xiàn)出了部分交互抗性,對二元毒素產(chǎn)生菌C3-41 和B-CW-1 產(chǎn)生高水平的交互抗性。另外,我們比較了敏感的SLCq 和抗性的RLCq1/C3-41、RLCq4/Mtx1 品系在生物適合度方面的差異。結(jié)果表明,兩種抗性品系RLCq1/C3-41 和RLCq4/Mtx1 的繁殖力略有降低,但這兩種品系雌蚊的壽命卻明顯長于SLCq 品系。
[Abstract]:Mtx1 toxin is a kind of insecticidal toxin produced by Bacillus sphaericus in the growth stage of vegetative body. Its action mechanism and insecticidal activity are different from that of binary toxin, so it has potential application value. In this study, the post-lethal effect of Mtx1 toxin on Culex quinquefasciatus and its resistance to Culex quinquefasciatuswere studied. The results showed that the resistance of Culex quinquefasciatus and the resistance of Culex quinquefasciatus to Culex quinquefasciatus were studied. Bioassay showed that Mtx1 toxin had little toxicity to several Lepidoptera insects, but showed moderate toxicity to Aedes albopictus, and high toxicity to Culex pipiens quinquefasciatus with sensitive and binary toxin resistance. The values of E-TH21 frozen powder expressing Mtx1 toxin to sensitive Culex pipiens quinquefasciatus strain SLCq and binary toxin resistant strain RLCq1/C3-41 and RLCq2/IAB59 were 0.508 mg / L and 0.675 mg / L, respectively. The results showed that Mtx1 toxin had potential application value in controlling or slowing the resistance of Culex pipiens quinquefasciatus to binary toxin. Mtx1 not only has a specific toxicidal effect on the larva of the target mosquito, but also has a strong postlethal effect. After 48 hours of treatment of 2-3 instar Culex quinquefasciatus larvae with Mtx1 toxin and binary toxin, respectively, the survival larvae of Cucurbitus quinquefasciatus treated with sublethal concentration (LC10 / LC1 / 30 / LC50 / LC50) and LC98 (respectively) showed a significant continuation of death and injury at all stages of growth. The death rate before Eclosion was 85.0% and 98.65%, and 99.50.100% and 23.1%, 44.4% and 61.6%, respectively. The toxin had a stronger post-lethal effect on Culex pipiens quinquefasciatus than the binary toxin. In the study of the resistance of Culex pipiens quinquefasciatus to Mtx1 toxin, we screened Culex quinquefasciatus with high dose of Mtx1 toxin for 30 generations and obtained a low level resistant strain RLCq4 / Mtx1, which was about 223.2 times more resistant to Mtx1 toxin than that of sensitive mosquito. The strain did not produce cross resistance to Bacillus thuringiensis Israeli subspecies B. thuringiensis subsp. israelensis) and Bacillus sphaeroides LP1-G and 47 6B, but showed partial cross resistance to IAB59 strain and high level cross resistance to binary toxin producing bacteria C3-41 and B-CW-1. In addition, we compared the differences in biological fitness between sensitive SLCq and resistant RLCq1 / C3-41 / RLCq4 / Mtx1 strains. The results showed that the fecundity of RLCq1/C3-41 and RLCq4/Mtx1 decreased slightly, but the lifespan of female mosquitoes was longer than that of SLCq.
【學(xué)位授予單位】：中國科學(xué)院研究生院（武漢病毒研究所）
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R384

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