本文選題：多巴胺 + Fos蛋白　； 參考：《中國公共衛(wèi)生》2014年09期

【摘要】：目的探討5-甲基二氫丙環(huán)庚烯亞胺馬來酸(MK-801)對傷害性電刺激隱神經(jīng)引起的扣帶回前部(ACC)多巴胺含量與Fos蛋白表達影響;N-甲基-D-天冬氨酸(NMDA)受體可能參與隱神經(jīng)傷害性信息傳入引起的ACC多巴胺含量升高與Fos蛋白表達增強過程。方法 72只雄性Wistar大鼠,隨機分為對照組、假刺激組、電刺激組、MK-801低、高劑量組及生理鹽水對照組;將大鼠斷頭、取大腦右側(cè)ACC,勻漿,4℃、10 000 r/min離心20min,取上清液20μL,應用高效液相色譜-電化學檢測技術檢測ACC內(nèi)多巴胺含量;將ACC腦組織連續(xù)冠狀冰凍切片,用免疫組化技術測定Fos蛋白表達。結果與假刺激組比較,電刺激組大鼠ACC多巴胺含量[(24.89±2.50)ng/g]、Fos蛋白表達量(8.481 1±1.243 2)和陽性細胞百分比[(3.133 8±0.824 2)%]明顯升高;與電刺激組比較,0.5、1.0 mg/kg MK-801組大鼠ACC多巴胺含量[分別為(15.07±3.75)、(14.84±2.92)ng/g]、Fos蛋白表達量[分別為(7.107 8±0.610 7)、(7.037 5±0.411 8)]與陽性細胞百分比[分別為(2.173 9±0.466 8)、(2.047 6±0.361 3)%]明顯下降;提示MK-801對傷害性電刺激隱神經(jīng)引起的大鼠腦ACC多巴胺含量升高與Fos蛋白表達增強具有拮抗作用。結論 NMDA受體可能參與隱神經(jīng)傷害性信息傳入引起的ACC多巴胺含量升高與Fos蛋白表達增強過程。
[Abstract]:Objective to investigate the effects of 5-methyldihydropropoxyheptene maleate (MK-801) on the content of dopamine and the expression of Fos protein in anterior cingulate gyrus induced by nociceptive electrical stimulation of saphenous nerve. N- methyl-Daspartic acid (NMDA) receptor may be involved in the injury of saphenous nerve. The increase of ACC dopamine content and the increase of Fos protein expression were induced by harmful information afferent. Methods Seventy-two male Wistar rats were randomly divided into control group, sham stimulation group, electrical stimulation group, low and high dose MK-801 group and saline control group. The right side of the brain was collected and centrifuged for 20 minutes at 4 鈩，

本文編號：1873001