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  本文選題：血管性眩暈 + 椎-基底動脈供血不足��； 參考：《湖南中醫(yī)藥大學(xué)》2005年碩士論文

【摘要】： 目的:建立一種操作簡單、切實(shí)可行的具有病證結(jié)合特點(diǎn)的血管性眩暈癥氣虛血瘀型動物模型,并擬建立相對完備的行為、體征客觀定量的評估體系。 方法:選取健康日本大耳白兔16只(體重2.0±0.3kg/只),按體重由小到大編號,隨機(jī)數(shù)字表法分成兩組,其中造模組8只,對照組8只。首先將組織硬化劑注射至模型組家兔左側(cè)頸椎橫突軟組織,造成左側(cè)椎動脈狹窄、基底動脈及雙側(cè)椎動脈血流降低以建立慢性椎-基底動脈供血不足(VBI)模型;并在此基礎(chǔ)上采用饑餓、高脂飲食復(fù)合因素制作出氣虛血瘀證模型;然后將初步符合氣虛血瘀證的動物選出,用旋轉(zhuǎn)法誘發(fā)動物產(chǎn)生眩暈癥。通過觀察造模前后大耳白兔的行為、體征、體重、血液流變學(xué)、跳臺耗時及腦血流圖變化;比較模型組與正常組家兔腦干前庭神經(jīng)核組織切片、肝臟DNA含量、跳臺耗時;以跳臺耗時作為評估眩暈程度和持續(xù)時間的指標(biāo)。 結(jié)果:1.與正常組比較,模型組腦干前庭神經(jīng)核組織切片神經(jīng)結(jié)構(gòu)基本正常,并沒有呈現(xiàn)急性缺血、缺氧樣改變;旋轉(zhuǎn)后跳臺耗時明顯延長,體征、體重、行為及肝臟DNA含量等氣虛血瘀證量化評分增高。2.造模前后相比,模型動物的腦血流量明顯下降,跳臺耗時明顯延長,血液流變學(xué)指標(biāo)、行為表現(xiàn)、體重等發(fā)生改變。 結(jié)論:該模型具有病證結(jié)合特點(diǎn),不僅能夠很好的反映眩暈程度及眩暈持續(xù)時間,而且能很好的反映中醫(yī)氣虛血瘀證候,可有效地應(yīng)用于抗血管性眩暈中藥的研究。經(jīng)2005年度文獻(xiàn)檢索查新尚未發(fā)現(xiàn)國內(nèi)外有相同的研究。
[Abstract]:Objective: to establish a simple and feasible animal model of vascular vertigo with Qi deficiency and blood stasis, and to establish an objective quantitative evaluation system of behavior and signs. Methods: sixteen Japanese white rabbits (weight 2.0 鹵0.3kg/) were randomly divided into two groups according to their weight from small to large, including 8 model group and 8 control group. First, the tissue sclerosing agent was injected into the soft tissue of the left cervical transverse process in the model group, which resulted in the stenosis of the left vertebral artery and the decrease of blood flow of the basilar artery and bilateral vertebral artery to establish the model of chronic vertebrobasilar artery insufficiency. On this basis, the combined factors of hunger and high fat diet were used to make the model of Qi deficiency and blood stasis syndrome, and then the animals that accord with Qi deficiency and blood stasis syndrome were selected, and the animals were induced vertigo by rotation method. The changes of behavior, physical signs, body weight, hemorheology, platform time and cerebral blood flow graph were observed before and after modeling, and the brain stem vestibular nucleus sections, liver DNA content, time consuming of platform jumping were compared between the model group and the normal group. The time spent on the platform is used as an index to evaluate the degree and duration of vertigo. The result is 1: 1. Compared with the normal group, the nerve structure of the brain stem vestibular nucleus in the model group was basically normal, without acute ischemia and anoxic changes. The quantitative score of Qi deficiency and blood stasis syndrome, such as behavior and liver DNA content, was increased by 2. 2. Compared with those before and after modeling, the cerebral blood flow of the model animals decreased obviously, the time consuming of jumping platform was prolonged, the hemorheology index, the behavior performance and the weight of the model animals were changed. Conclusion: the model can not only reflect the degree of vertigo and the duration of vertigo, but also reflect the syndrome of qi deficiency and blood stasis, and can be effectively used in the study of anti-vascular vertigo. The same research has not been found at home and abroad by literature retrieval in 2005.
【學(xué)位授予單位】：湖南中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2005
【分類號】：R-332

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本文編號：1870167