本文選題：TN-C + TN-X　； 參考：《南京醫(yī)科大學(xué)》2007年碩士論文

【摘要】： 目的：檢測韌黏素-C(TN-C)和韌黏素-X(TN-X)在不同孕齡大鼠胚胎心臟中的表達(dá)規(guī)律，探討其在大鼠胚胎心臟發(fā)育過程中的作用。 方法：60只雌性SD大鼠，24只雄性SD大鼠，雌雄同籠，以觀察到雌鼠排出陰栓為妊娠0.5天(ED0.5)，分別于ED11.5、ED12.5、ED13.5、ED15.5、ED17.5、ED19.5、新生1天處死孕鼠或新生鼠，取出心臟或全胚。用半定量逆轉(zhuǎn)錄聚合酶鏈反應(yīng)(RT-PCR)測定TN-C和TN-X在各孕齡的胚胎心臟中mRNA的表達(dá)水平；免疫組織化學(xué)染色法檢測上述各孕齡大鼠心臟TN-C和TN-X蛋白的表達(dá)；免疫印跡法(Western blot)檢測TN-C在大鼠胚胎心臟中的表達(dá)水平，進(jìn)一步驗(yàn)證免疫組化的結(jié)果。 結(jié)果：TN-C mRNA和蛋白在大鼠胚胎心臟發(fā)育早期有豐富表達(dá)，于ED15.5即顯著下調(diào)；TN-X mRNA在大鼠胚胎ED12.5開始表達(dá)上調(diào)，其后各孕齡表達(dá)未見明顯差異。免疫組化顯示，TN-C在ED11.5、ED12.5、ED13.5表達(dá)于心臟流出道、前心外膜器官、房室墊和心包腔，而在ED15.5、ED17.5、ED19.5、新生1天相應(yīng)表達(dá)為陰性；TN-X在各孕齡胚胎心臟中均有表達(dá)，且隨著胎齡增大，表達(dá)范圍更廣泛，于新生1天的大鼠心臟中也廣泛表達(dá)。 結(jié)論：TN-C在大鼠胚胎心臟發(fā)育早期表達(dá)豐富，對心臟的早期發(fā)育有一定關(guān)系；而TN-X在ED11.5于形成血管的心外膜細(xì)胞周圍表達(dá)，提示TN-X可能與心臟早期冠脈等血管的形成有關(guān)。
[Abstract]:Aim: to detect the expression of tensionin-Con-TN-C) and ductile-XTN-X) in rat embryonic heart of different gestational ages and to explore the role of them in the development of rat embryonic heart. Methods Twenty four male Sprague-Dawley rats with 60 female Sprague-Dawley rats were treated in the same cage. It was observed that the female rats excreted negative suppository as 0.5 day gestation ED0.5, ED11.5 ED12.5 ED15.5ED15.5ED17.5ED19.5. on the first day of birth, pregnant rats or newborn rats were killed and the heart or whole embryo was taken out. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of TN-C and TN-X in the fetal heart of each gestational age, and the expression of TN-C and TN-X protein in the heart of each gestational age was detected by immunohistochemical staining. Western blots were used to detect the expression of TN-C in rat embryonic heart. Results there were abundant expression of TN-C mRNA and protein in the early stage of embryonic heart development in rats. After ED15.5, the expression of TN-X mRNA began to up-regulate in rat embryo ED12.5, but there was no significant difference in the expression of TN-C mRNA and protein in different gestational ages. Immunohistochemical staining showed that TN-C was expressed in cardiac outflow tract, anterior epicardial organ, atrioventricular pad and pericardium, and ED19.5at ED15.5ED17.5. at the first day of birth, TN-X was expressed in the heart of every gestational age, and it increased with gestational age. The expression was more widespread and was also widely expressed in the heart of a day-old rat. Conclusion the expression of TN-C is abundant in the early stage of embryonic heart development in rats, which may be related to the early development of the heart, while TN-X is expressed around the epicardial cells that form blood vessels, suggesting that TN-X may be related to the formation of coronary arteries and other vessels in the early stage of the heart.
【學(xué)位授予單位】：南京醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R321;R725.4

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本文編號：1853506