本文選題：窒息法 + 心肺復(fù)蘇模型�。� 參考：《蘇州大學(xué)》2006年碩士論文

【摘要】：第一部分 窒息大鼠心肺復(fù)蘇模型的建立 目的：建立窒息大鼠心肺復(fù)蘇模型；探討心臟驟停(Cardiac arrest，CA)持續(xù)時(shí)間對(duì)復(fù)蘇效果的影響。 方法：雄性SD大鼠20只，隨機(jī)分為2組：CA1min組；CA3min組。通過(guò)靜脈注射肌松劑司可林模擬窒息的方法，使大鼠心臟驟停。心臟停跳持續(xù)1min或3min后實(shí)施心肺復(fù)蘇，應(yīng)用心電監(jiān)護(hù)儀監(jiān)測(cè)兩組大鼠心肺復(fù)蘇期間MAP、HR及體溫的動(dòng)態(tài)變化，股動(dòng)脈采血監(jiān)測(cè)血?dú)獾膭?dòng)態(tài)變化，并觀察兩組大鼠自主循環(huán)恢復(fù)時(shí)間、復(fù)蘇成功率及復(fù)蘇后3小時(shí)的存活情況。 結(jié)果：1．CA1min和CA3min兩組大鼠心肺復(fù)蘇期間各項(xiàng)基本生命指征的變化趨勢(shì)基本相同；但開始復(fù)蘇之前，CA3min組大鼠的MAP和HR均明顯低于CA1min組的大鼠(P＜0.05)，接近于0；兩組大鼠ROSC時(shí)均存在較嚴(yán)重的代謝性酸中毒，提示此時(shí)補(bǔ)堿的必要性。 2．CA3min組大鼠的ROSC時(shí)間較CA1min組明顯增加(P＜0.05)，，其復(fù)蘇后3小時(shí)存活率較CA1min組明顯降低(P＜0.05)。CA3min組的ROSC率與CA1min組比較，雖無(wú)顯著性差異，但呈下降趨勢(shì)。 結(jié)論：1．成功建立窒息大鼠心肺復(fù)蘇模型，模型基本穩(wěn)定。 2．CA的持續(xù)時(shí)間及ROSC時(shí)間是決定心肺復(fù)蘇成敗及復(fù)蘇預(yù)后的重要因素。CA時(shí)間及ROSC時(shí)間越長(zhǎng)，復(fù)蘇后長(zhǎng)期存活率越低。 第二部分 心肺復(fù)蘇早期大鼠腦組織含水量和水通道蛋白-4的動(dòng)態(tài)變化及亞低溫對(duì)其影響的研究 目的：探討復(fù)蘇后早期腦水腫的動(dòng)態(tài)變化，AQP4在其發(fā)生、發(fā)展中的作用，以及亞低溫干預(yù)對(duì)二者的影響。 方法：雄性SD大鼠48只，隨機(jī)分為4組，分別為正常對(duì)照組、假手術(shù)組、常溫復(fù)蘇組、亞低溫組，后兩組各自進(jìn)一步分為3個(gè)亞組：自主循環(huán)恢復(fù)后1、3和6小時(shí)組。建立窒息大鼠心肺復(fù)蘇模型，應(yīng)用干濕重法測(cè)定自主循環(huán)恢復(fù)后1、
[Abstract]:Part I Establishment of cardiopulmonary resuscitation model in asphyxia rats Aim: to establish cardiopulmonary resuscitation (CPR) model in asphyxiated rats and to investigate the effect of cardiac arrest CAA on resuscitation. Methods: twenty male Sprague-Dawley rats were randomly divided into 2 groups. Cardiac arrest was induced by intravenous injection of muscle relaxant Secolin to simulate asphyxia in rats. Cardiopulmonary resuscitation (CPR) was performed after cardiopulmonary resuscitation (1min) or cardiopulmonary resuscitation (3min). The dynamic changes of MAPHR and body temperature during cardiopulmonary resuscitation (CPR) were monitored in the two groups. The success rate of resuscitation and survival rate of 3 hours after resuscitation. Results 1. The changes of basic life indications in CA1min group and CA3min group were basically the same during cardiopulmonary resuscitation (CPR). Before resuscitation, the MAP and HR of rats in CA3min group were significantly lower than those in CA1min group (P < 0.05), and there were severe metabolic acidosis at ROSC in both groups, indicating the necessity of alkali supplementation. The ROSC time in 2.CA3min group was significantly higher than that in CA1min group (P < 0.05). The survival rate at 3 hours after resuscitation was significantly lower in 2.CA3min group than in CA1min group, and the ROSC rate in P < 0.05).CA3min group was significantly lower than that in CA1min group. Conclusion 1. The model of cardiopulmonary resuscitation in asphyxiated rats was established successfully, and the model was basically stable. The duration of 2.CA and the duration of ROSC were important factors to determine the success or failure of CPR and prognosis of CPR. The longer the duration of CA and ROSC, the lower the long-term survival rate after resuscitation. The second part: the dynamic changes of water content and aquaporin-4 in brain tissue of rats at the early stage of cardiopulmonary resuscitation and the effect of mild hypothermia on them. Aim: to investigate the dynamic changes of brain edema in the early stage after resuscitation and the role of AQP4 in the pathogenesis and development of brain edema and the effect of mild hypothermia intervention on them. Methods: Forty-eight male SD rats were randomly divided into 4 groups: normal control group, sham operation group, normothermic resuscitation group and mild hypothermia group. The cardiopulmonary resuscitation model of asphyxia rats was established.
【學(xué)位授予單位】：蘇州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R-332

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