本文選題：GSTT1 + GSTM1��； 參考：《廣西醫(yī)科大學(xué)》2006年碩士論文

【摘要】： 目的:探討代謝相關(guān)基因GSTT1、GSTM1、CYP1A1及抑癌基因p53第4外顯子第72位密碼子(codon72)與人類長壽的關(guān)系。方法:應(yīng)用PCR-RFLP技術(shù)對(duì)巴馬156例(年齡90-105歲,平均93.2歲,長壽組)健康壯族長壽老人和142例健康壯族成年人(年齡20-72歲,平均34.8歲,對(duì)照組)的GSTT1、GSTM1、CYP1A1及p53基因進(jìn)行基因分型,了解這些基因的多態(tài)性,分析各種基因(型)頻率及多態(tài)性組合在長壽組和對(duì)照組之間的差異及其與長壽的關(guān)系。結(jié)果:長壽組的GSTT1基因、GSTM1基因的缺失(或攜帶)頻率、CYP1A1基因的野生型(T/T)、純合突變型(T/C)、雜合突變型(C/C)頻率與對(duì)照組差別均無顯著意義(p0.05);長壽組p53codon72片段的野生型(G/G)頻率明顯高于對(duì)照組(53%:29%,p0.05)。各種基因型的組合分析顯示,對(duì)照組同時(shí)合并GSTT1、GSTM1、CYP1A1及p53codon72基因缺失或者突變的幾率明顯比長壽組為高,尤其是長壽組男性和女性的GSTT1(+)-GSTM1(-)-CYP1A1 (T/C,C/C)- p53coden72(G/C,C/C)基因組合及長壽組女性GSTT1(-)-GSTM1(-)-CYP1A1 (T/C,C/C)-p53coden72 (G/C,C/C)基因組合明顯低于對(duì)照組( p0.05 );而長壽組男性GSTT1(-)-GSTM1(-)-CYP1A1 (T/C,C/C)-p53coden72 (G/G)基因組合高于對(duì)照組(p0.05),其它基因(型)組合在兩組中無差別。結(jié)論:GSTT1、GSTM1、CYP1A1及p53基因的多態(tài)性與人類長壽有著較為密切的聯(lián)系,其具體作用機(jī)制有待進(jìn)一步研究。
[Abstract]:Aim: to investigate the relationship between the metabolism-related gene GSTT1GSTM1CY CYP1A1 and codon 72of p53 exon 4. Methods: genotyping of CYP1A1 and p53 genes in 156 healthy adults (aged 20-72 years, mean 34.8 years, control group) from 156 healthy adults (90-105 years old, average 93.2 years old) and 142 healthy adults (20-72 years old, mean 34.8 years old, control group) was performed using PCR-RFLP technique. To understand the polymorphism of these genes, and to analyze the differences between longevity group and control group and their relationship with longevity. Results: there was no significant difference in the frequency of deletion (or carrier) of GSTT1 gene, wild type of CYP1A1 gene, homozygous mutant T / C, heterozygous mutation type C / C between the longevity group and the control group (p 0.05), while the wild type G / G) frequency of the p53codon72 fragment in the longevity group was not significantly different from that in the control group. The rate was significantly higher than that of the control group. Combination analysis of different genotypes showed that the rate of deletion or mutation of CYP1A1 and p53codon72 gene in the control group was significantly higher than that in the longevity group. 灝ゅ叾鏄暱瀵跨粍鐢鋒，

本文編號(hào)：1839522