本文選題：原位肝移植 + 大鼠��； 參考：《天津醫(yī)科大學》2006年碩士論文

【摘要】：目的:肝移植是各種終末期肝病的最有效治療方法,而急性排斥反應又是肝移植后最常見的并發(fā)癥和死亡原因。大鼠是移植研究中一種較為優(yōu)越的實驗動物,我們參照國內(nèi)外大鼠肝移植手術方法并加以改進,擬建立一種簡易、可靠的動物模型以供臨床進一步研究。目前就急性排斥反應的診斷而言,目前尚缺乏特異性及敏感性均令人滿意的診斷方法。本研究旨在通過檢測肝移植大鼠外周血中細胞因子IFN-γ的含量,以探討它們對大鼠肝移植后急性排斥反應的診斷和預測價值。 方法:采用“二袖套管法”,通過建立Wistar-Wistar大鼠肝移植模型熟練建立大鼠肝移植模型的技能。繼而將實驗大鼠共分2組,每組均為15只大鼠,研究同種異體組(SD-Wistar組)、同基因組(Wistar—Wistar組)組的肝移植后各項指標,分析各組大鼠死亡原因,觀察術后不同階段各組癥狀體征、肝功能變化、移植肝病理特征以及細胞因子IFN-γ等的變化。 結果:經(jīng)過熟悉后,我們在第二階段共行大鼠原位肝移植實驗37次,手術成功率為91.9%。同種異體組的大鼠平均生存時間為25天,而同基因組大鼠可長期存活,幾乎無排斥反應,且術后肝功能恢復迅速。同種異體組肝功能明顯受損,且恢復緩慢,病理檢查可見明顯排斥反應。同種異體組大鼠外周血中IFN-γ的含量明顯高于同基因組(P0.01),有明顯統(tǒng)計學意義。 結論:成功建立了大鼠原位肝移植的模型并加以改進。肝移植模型的特點:①肝上下腔靜脈采取直接縫合;②肝下下腔靜脈、門靜脈套管吻合,縮短了無肝期時間;③套管自制,節(jié)省了費用,并且可以根據(jù)不同血管管徑選擇不同套管。完善的手術技術是建立穩(wěn)定肝移植模型的關鍵。SD-wistar肝移植模型鼠有明顯急性排斥反應的特征,Wistar-Wistar肝移植模型鼠幾無排斥反應發(fā)生,
[Abstract]:Objective: liver transplantation is the most effective treatment for various end-stage liver diseases, and acute rejection is the most common complication and cause of death after liver transplantation. Rat is a superior experimental animal in transplantation research. We propose to establish a simple and reliable animal model for further clinical study by referring to and improving the methods of liver transplantation in rats at home and abroad. For the diagnosis of acute rejection, there is no satisfactory diagnostic method of specificity and sensitivity. The purpose of this study was to investigate the diagnostic and predictive value of cytokines IFN- 緯 in peripheral blood of liver transplantation rats. Methods: two sleeve cannula method was used to establish Wistar-Wistar rat liver transplantation model skillfully. Then the experimental rats were divided into two groups, 15 rats in each group. The indexes of liver transplantation in SD-Wistar group and Wistar Wistar group were studied, and the causes of death in each group were analyzed, and the symptoms and signs of each group were observed at different stages after operation. Changes of liver function, pathological features and cytokines IFN- 緯 of transplanted liver. Results: after familiarity, we performed 37 orthotopic liver transplantation experiments in the second stage, and the success rate was 91.9%. The average survival time of allogeneic rats was 25 days, while the same genome rats could survive for a long time, almost no rejection, and the liver function recovered rapidly after operation. The liver function of allograft group was obviously damaged, and the recovery was slow, and the rejection was obvious in pathological examination. The level of IFN- 緯 in peripheral blood of allogeneic group was significantly higher than that of homogenetic group (P 0.01). Conclusion: the rat orthotopic liver transplantation model was successfully established and improved. The characteristics of liver transplantation model were as follows: direct suture of inferior inferior vena cava via direct suture of inferior vena cava, portal venous cannula anastomosis, shortening the time of anhepatic stage and saving cost. Different cannula can be selected according to different vessel diameter. Perfect surgical technique is the key to establish stable liver transplantation model. SD-wistar liver transplantation model mice have obvious acute rejection characteristics. There is almost no rejection in Wistar Wistar liver transplantation model rats.
【學位授予單位】：天津醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2006
【分類號】：R-332

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