大鼠原位肝移植及急性排斥反應(yīng)實(shí)驗(yàn)?zāi)Ｐ徒⒌某醪窖芯?/H1>
發(fā)布時(shí)間：2018-05-03 14:27
本文選題：原位肝移植 + 大鼠��； 參考：《天津醫(yī)科大學(xué)》2006年碩士論文
【摘要】：目的:肝移植是各種終末期肝病的最有效治療方法,而急性排斥反應(yīng)又是肝移植后最常見的并發(fā)癥和死亡原因。大鼠是移植研究中一種較為優(yōu)越的實(shí)驗(yàn)動(dòng)物,我們參照國內(nèi)外大鼠肝移植手術(shù)方法并加以改進(jìn),擬建立一種簡(jiǎn)易、可靠的動(dòng)物模型以供臨床進(jìn)一步研究。目前就急性排斥反應(yīng)的診斷而言,目前尚缺乏特異性及敏感性均令人滿意的診斷方法。本研究旨在通過檢測(cè)肝移植大鼠外周血中細(xì)胞因子IFN-γ的含量,以探討它們對(duì)大鼠肝移植后急性排斥反應(yīng)的診斷和預(yù)測(cè)價(jià)值。 方法:采用“二袖套管法”,通過建立Wistar-Wistar大鼠肝移植模型熟練建立大鼠肝移植模型的技能。繼而將實(shí)驗(yàn)大鼠共分2組,每組均為15只大鼠,研究同種異體組(SD-Wistar組)、同基因組(Wistar—Wistar組)組的肝移植后各項(xiàng)指標(biāo),分析各組大鼠死亡原因,觀察術(shù)后不同階段各組癥狀體征、肝功能變化、移植肝病理特征以及細(xì)胞因子IFN-γ等的變化。 結(jié)果:經(jīng)過熟悉后,我們?cè)诘诙A段共行大鼠原位肝移植實(shí)驗(yàn)37次,手術(shù)成功率為91.9%。同種異體組的大鼠平均生存時(shí)間為25天,而同基因組大鼠可長(zhǎng)期存活,幾乎無排斥反應(yīng),且術(shù)后肝功能恢復(fù)迅速。同種異體組肝功能明顯受損,且恢復(fù)緩慢,病理檢查可見明顯排斥反應(yīng)。同種異體組大鼠外周血中IFN-γ的含量明顯高于同基因組(P0.01),有明顯統(tǒng)計(jì)學(xué)意義。 結(jié)論:成功建立了大鼠原位肝移植的模型并加以改進(jìn)。肝移植模型的特點(diǎn):①肝上下腔靜脈采取直接縫合;②肝下下腔靜脈、門靜脈套管吻合,縮短了無肝期時(shí)間;③套管自制,節(jié)省了費(fèi)用,并且可以根據(jù)不同血管管徑選擇不同套管。完善的手術(shù)技術(shù)是建立穩(wěn)定肝移植模型的關(guān)鍵。SD-wistar肝移植模型鼠有明顯急性排斥反應(yīng)的特征,Wistar-Wistar肝移植模型鼠幾無排斥反應(yīng)發(fā)生,
[Abstract]:Objective: liver transplantation is the most effective treatment for various end-stage liver diseases, and acute rejection is the most common complication and cause of death after liver transplantation. Rat is a superior experimental animal in transplantation research. We propose to establish a simple and reliable animal model for further clinical study by referring to and improving the methods of liver transplantation in rats at home and abroad. For the diagnosis of acute rejection, there is no satisfactory diagnostic method of specificity and sensitivity. The purpose of this study was to investigate the diagnostic and predictive value of cytokines IFN- 緯 in peripheral blood of liver transplantation rats. Methods: two sleeve cannula method was used to establish Wistar-Wistar rat liver transplantation model skillfully. Then the experimental rats were divided into two groups, 15 rats in each group. The indexes of liver transplantation in SD-Wistar group and Wistar Wistar group were studied, and the causes of death in each group were analyzed, and the symptoms and signs of each group were observed at different stages after operation. Changes of liver function, pathological features and cytokines IFN- 緯 of transplanted liver. Results: after familiarity, we performed 37 orthotopic liver transplantation experiments in the second stage, and the success rate was 91.9%. The average survival time of allogeneic rats was 25 days, while the same genome rats could survive for a long time, almost no rejection, and the liver function recovered rapidly after operation. The liver function of allograft group was obviously damaged, and the recovery was slow, and the rejection was obvious in pathological examination. The level of IFN- 緯 in peripheral blood of allogeneic group was significantly higher than that of homogenetic group (P 0.01). Conclusion: the rat orthotopic liver transplantation model was successfully established and improved. The characteristics of liver transplantation model were as follows: direct suture of inferior inferior vena cava via direct suture of inferior vena cava, portal venous cannula anastomosis, shortening the time of anhepatic stage and saving cost. Different cannula can be selected according to different vessel diameter. Perfect surgical technique is the key to establish stable liver transplantation model. SD-wistar liver transplantation model mice have obvious acute rejection characteristics. There is almost no rejection in Wistar Wistar liver transplantation model rats.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R-332
【相似文獻(xiàn)】
相關(guān)期刊論文 前10條
1 張明鈞,嚴(yán)佶祺,蔡偉耀,楊衛(wèi)平,李宏為;術(shù)前營養(yǎng)狀態(tài)影響原位肝移植存活率的實(shí)驗(yàn)研究[J];外科理論與實(shí)踐;2000年01期
2 劉立新,彭方興,張英男,陳哲宇,嚴(yán)律南;改良法大鼠原位肝移植死亡因素分析及對(duì)策[J];中國普外基礎(chǔ)與臨床雜志;2005年02期
3 朱新華;仇毓東;史敏科;丁義濤;;苦參堿對(duì)大鼠原位肝移植冷缺血再灌注損傷的保護(hù)作用[J];世界華人消化雜志;2006年17期
4 蔡明;潘桃;李紅洲;陳知水;;大鼠原位肝移植手術(shù)技巧探討[J];醫(yī)學(xué)研究生學(xué)報(bào);2009年01期
5 祖存;王培紅;謝建國;范偉;李波;;改良二袖套法大鼠原位肝移植模型的建立[J];西南國防醫(yī)藥;2005年06期
6 彭方興,鄭本波,劉立新,權(quán)毅,智星,楊家印,嚴(yán)律南;大鼠原位肝移植動(dòng)物模型制作要點(diǎn)[J];中國普外基礎(chǔ)與臨床雜志;2005年02期
7 李國林;龍?zhí)熘?林浩銘;呂麗虹;徐捚耀;俞建東;許曉婷;閔軍;萬云樂;;大鼠原位肝移植膽道并發(fā)癥的原因分析[J];嶺南現(xiàn)代臨床外科;2009年02期
8 梁四海;陶立德;張培建;王懋莉;馮敏;劉新顏;張淼;;低氧預(yù)適應(yīng)對(duì)大鼠移植肝臟功能和組織結(jié)構(gòu)的影響[J];江蘇大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2007年03期
9 彭勇,龔建平,劉明忠,劉海忠,尤海波,劉長(zhǎng)安;改進(jìn)的大鼠原位肝移植術(shù)與相關(guān)手術(shù)技巧[J];重慶醫(yī)科大學(xué)學(xué)報(bào);2003年06期
10 李軍;竇科峰;趙青川;張洪濤;孔亞林;張福琴;;兩套袖法大鼠原位肝移植模型的建立及改進(jìn)[J];第四軍醫(yī)大學(xué)學(xué)報(bào);2006年09期
相關(guān)會(huì)議論文 前10條
1 程井軍;吳其愷;彭銳;孫國杰;;電針對(duì)非酒精性脂肪性肝炎大鼠肝組織氧化及抗氧化狀態(tài)的影響[A];中華中醫(yī)藥學(xué)會(huì)第十三屆內(nèi)科肝膽病學(xué)術(shù)會(huì)議論文匯編[C];2008年
2 楊s，
本文編號(hào)：1838874
本文鏈接：http://sikaile.net/yixuelunwen/binglixuelunwen/1838874.html
本文選題：原位肝移植 + 大鼠��； 參考：《天津醫(yī)科大學(xué)》2006年碩士論文
【摘要】：目的:肝移植是各種終末期肝病的最有效治療方法,而急性排斥反應(yīng)又是肝移植后最常見的并發(fā)癥和死亡原因。大鼠是移植研究中一種較為優(yōu)越的實(shí)驗(yàn)動(dòng)物,我們參照國內(nèi)外大鼠肝移植手術(shù)方法并加以改進(jìn),擬建立一種簡(jiǎn)易、可靠的動(dòng)物模型以供臨床進(jìn)一步研究。目前就急性排斥反應(yīng)的診斷而言,目前尚缺乏特異性及敏感性均令人滿意的診斷方法。本研究旨在通過檢測(cè)肝移植大鼠外周血中細(xì)胞因子IFN-γ的含量,以探討它們對(duì)大鼠肝移植后急性排斥反應(yīng)的診斷和預(yù)測(cè)價(jià)值。 方法:采用“二袖套管法”,通過建立Wistar-Wistar大鼠肝移植模型熟練建立大鼠肝移植模型的技能。繼而將實(shí)驗(yàn)大鼠共分2組,每組均為15只大鼠,研究同種異體組(SD-Wistar組)、同基因組(Wistar—Wistar組)組的肝移植后各項(xiàng)指標(biāo),分析各組大鼠死亡原因,觀察術(shù)后不同階段各組癥狀體征、肝功能變化、移植肝病理特征以及細(xì)胞因子IFN-γ等的變化。 結(jié)果:經(jīng)過熟悉后,我們?cè)诘诙A段共行大鼠原位肝移植實(shí)驗(yàn)37次,手術(shù)成功率為91.9%。同種異體組的大鼠平均生存時(shí)間為25天,而同基因組大鼠可長(zhǎng)期存活,幾乎無排斥反應(yīng),且術(shù)后肝功能恢復(fù)迅速。同種異體組肝功能明顯受損,且恢復(fù)緩慢,病理檢查可見明顯排斥反應(yīng)。同種異體組大鼠外周血中IFN-γ的含量明顯高于同基因組(P0.01),有明顯統(tǒng)計(jì)學(xué)意義。 結(jié)論:成功建立了大鼠原位肝移植的模型并加以改進(jìn)。肝移植模型的特點(diǎn):①肝上下腔靜脈采取直接縫合;②肝下下腔靜脈、門靜脈套管吻合,縮短了無肝期時(shí)間;③套管自制,節(jié)省了費(fèi)用,并且可以根據(jù)不同血管管徑選擇不同套管。完善的手術(shù)技術(shù)是建立穩(wěn)定肝移植模型的關(guān)鍵。SD-wistar肝移植模型鼠有明顯急性排斥反應(yīng)的特征,Wistar-Wistar肝移植模型鼠幾無排斥反應(yīng)發(fā)生,
[Abstract]:Objective: liver transplantation is the most effective treatment for various end-stage liver diseases, and acute rejection is the most common complication and cause of death after liver transplantation. Rat is a superior experimental animal in transplantation research. We propose to establish a simple and reliable animal model for further clinical study by referring to and improving the methods of liver transplantation in rats at home and abroad. For the diagnosis of acute rejection, there is no satisfactory diagnostic method of specificity and sensitivity. The purpose of this study was to investigate the diagnostic and predictive value of cytokines IFN- 緯 in peripheral blood of liver transplantation rats. Methods: two sleeve cannula method was used to establish Wistar-Wistar rat liver transplantation model skillfully. Then the experimental rats were divided into two groups, 15 rats in each group. The indexes of liver transplantation in SD-Wistar group and Wistar Wistar group were studied, and the causes of death in each group were analyzed, and the symptoms and signs of each group were observed at different stages after operation. Changes of liver function, pathological features and cytokines IFN- 緯 of transplanted liver. Results: after familiarity, we performed 37 orthotopic liver transplantation experiments in the second stage, and the success rate was 91.9%. The average survival time of allogeneic rats was 25 days, while the same genome rats could survive for a long time, almost no rejection, and the liver function recovered rapidly after operation. The liver function of allograft group was obviously damaged, and the recovery was slow, and the rejection was obvious in pathological examination. The level of IFN- 緯 in peripheral blood of allogeneic group was significantly higher than that of homogenetic group (P 0.01). Conclusion: the rat orthotopic liver transplantation model was successfully established and improved. The characteristics of liver transplantation model were as follows: direct suture of inferior inferior vena cava via direct suture of inferior vena cava, portal venous cannula anastomosis, shortening the time of anhepatic stage and saving cost. Different cannula can be selected according to different vessel diameter. Perfect surgical technique is the key to establish stable liver transplantation model. SD-wistar liver transplantation model mice have obvious acute rejection characteristics. There is almost no rejection in Wistar Wistar liver transplantation model rats.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R-332
【相似文獻(xiàn)】
相關(guān)期刊論文 前10條
1 張明鈞,嚴(yán)佶祺,蔡偉耀,楊衛(wèi)平,李宏為;術(shù)前營養(yǎng)狀態(tài)影響原位肝移植存活率的實(shí)驗(yàn)研究[J];外科理論與實(shí)踐;2000年01期
2 劉立新,彭方興,張英男,陳哲宇,嚴(yán)律南;改良法大鼠原位肝移植死亡因素分析及對(duì)策[J];中國普外基礎(chǔ)與臨床雜志;2005年02期
3 朱新華;仇毓東;史敏科;丁義濤;;苦參堿對(duì)大鼠原位肝移植冷缺血再灌注損傷的保護(hù)作用[J];世界華人消化雜志;2006年17期
4 蔡明;潘桃;李紅洲;陳知水;;大鼠原位肝移植手術(shù)技巧探討[J];醫(yī)學(xué)研究生學(xué)報(bào);2009年01期
5 祖存;王培紅;謝建國;范偉;李波;;改良二袖套法大鼠原位肝移植模型的建立[J];西南國防醫(yī)藥;2005年06期
6 彭方興,鄭本波,劉立新,權(quán)毅,智星,楊家印,嚴(yán)律南;大鼠原位肝移植動(dòng)物模型制作要點(diǎn)[J];中國普外基礎(chǔ)與臨床雜志;2005年02期
7 李國林;龍?zhí)熘?林浩銘;呂麗虹;徐捚耀;俞建東;許曉婷;閔軍;萬云樂;;大鼠原位肝移植膽道并發(fā)癥的原因分析[J];嶺南現(xiàn)代臨床外科;2009年02期
8 梁四海;陶立德;張培建;王懋莉;馮敏;劉新顏;張淼;;低氧預(yù)適應(yīng)對(duì)大鼠移植肝臟功能和組織結(jié)構(gòu)的影響[J];江蘇大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2007年03期
9 彭勇,龔建平,劉明忠,劉海忠,尤海波,劉長(zhǎng)安;改進(jìn)的大鼠原位肝移植術(shù)與相關(guān)手術(shù)技巧[J];重慶醫(yī)科大學(xué)學(xué)報(bào);2003年06期
10 李軍;竇科峰;趙青川;張洪濤;孔亞林;張福琴;;兩套袖法大鼠原位肝移植模型的建立及改進(jìn)[J];第四軍醫(yī)大學(xué)學(xué)報(bào);2006年09期
相關(guān)會(huì)議論文 前10條
1 程井軍;吳其愷;彭銳;孫國杰;;電針對(duì)非酒精性脂肪性肝炎大鼠肝組織氧化及抗氧化狀態(tài)的影響[A];中華中醫(yī)藥學(xué)會(huì)第十三屆內(nèi)科肝膽病學(xué)術(shù)會(huì)議論文匯編[C];2008年
2 楊s，
本文編號(hào)：1838874
本文鏈接：http://sikaile.net/yixuelunwen/binglixuelunwen/1838874.html
