本文選題：氟化鈉 + 鋅��； 參考：《廣西大學(xué)》2007年碩士論文

【摘要】： 本研究通過(guò)對(duì)雄性小鼠腹腔注射氟化鈉及醋酸鋅建立氟中毒動(dòng)物模型和治療模型，采用石蠟切片、H-E染色、TUNEL末端原位標(biāo)記及瓊脂糖凝膠電泳的方法檢測(cè)小鼠睪丸生精細(xì)胞的凋亡情況。旨在研究氟對(duì)成年小鼠生精細(xì)胞凋亡的影響及鋅的保護(hù)作用，從而對(duì)基礎(chǔ)理論研究及開(kāi)發(fā)低毒鼠藥以控制鼠害提供實(shí)驗(yàn)依據(jù)，并對(duì)經(jīng)常接觸氟的人群的生殖保健起到一定的參考指導(dǎo)作用。 實(shí)驗(yàn)結(jié)果如下：小鼠腹腔注射氟化鈉(20mgkg~(1-))后，睪丸生精細(xì)胞的顯微結(jié)構(gòu)出現(xiàn)了典型的細(xì)胞凋亡的形態(tài)特點(diǎn)。 TUNEL法顯示，小鼠睪丸中凋亡細(xì)胞呈陽(yáng)性反應(yīng)，非凋亡細(xì)胞呈陰性反應(yīng)。 無(wú)論是對(duì)照組還是氟感染組和鋅保護(hù)組，睪丸中凋亡的生精細(xì)胞多發(fā)生在精母細(xì)胞和精原細(xì)胞，而且是精母細(xì)胞凋亡最多；很少發(fā)生在精子細(xì)胞和精子。 從細(xì)胞凋亡率可見(jiàn)，染氟組睪丸生精細(xì)胞細(xì)胞凋亡率顯著高于對(duì)照組；染氟組的生精細(xì)胞凋亡率在腹腔注射第28天達(dá)到最大(11.972％)，以后逐漸回落。低劑量鋅組(15mgkg~(-1))和高劑量鋅組(30mgkg~(1-))的生精細(xì)胞凋亡率顯著低于染氟組，并且高劑量鋅組的凋亡指數(shù)與對(duì)照組差異不顯著(P＞0.05)。 瓊脂糖凝膠電泳檢測(cè)顯示，對(duì)照組及鋅保護(hù)組細(xì)胞DNA條帶呈單一條帶。染氟組凋亡細(xì)胞的DNA呈現(xiàn)特征性的“階梯狀”條帶。 可見(jiàn)，，氟化鈉可以在體內(nèi)誘導(dǎo)小鼠睪丸生精細(xì)胞凋亡；鋅可以對(duì)這種凋亡起到保護(hù)作用。
[Abstract]:In this study, the animal model and treatment model were established by intraperitoneal injection of sodium fluoride and zinc acetate in male mice. The apoptosis of spermatogenic cells in testis of mice was detected by paraffin section, H-E staining, TUNEL terminal in situ labeling and agarose gel electrophoresis, and the effect of fluorine on the apoptosis of spermatogenic cells in adult mice was studied. The protective effect of zinc can provide experimental basis for basic theory research and development of low toxic rodent drugs to control rodent damage, and play a certain reference role to reproductive health care for people who often contact fluorine.
The results were as follows: after injection of sodium fluoride (20mgkg~ (1-)), the morphology of spermatogenic cells in the testis showed typical morphological characteristics of apoptosis.
TUNEL showed that the apoptotic cells in the testis of mice were positive, and the non apoptotic cells were negative.
The apoptotic spermatogonial cells in the testis were mostly in spermatocytes and spermatogonial cells in the control group or in the fluorine infection group and the zinc protective group, and the apoptosis was the most in spermatocytes, and the spermatocytes and spermatozoa were rarely occurring.
The apoptosis rate of testicular spermatogenic cells in the fluoride group was significantly higher than that in the control group. The apoptosis rate of spermatogenic cells in the fluorine group reached the maximum (11.972%) after twenty-eighth days of intraperitoneal injection (15mgkg~ (-1)) and the high dose zinc group (30mgkg~ (1-)) was significantly lower than that of the fluorine dyed group. There was no significant difference in the apoptotic index between the high-dose zinc group and the control group (P > 0.05).
The agarose gel electrophoresis showed that the DNA bands in the control group and the zinc protective group showed a single band. The DNA of the apoptotic cells in the fluorine stained group showed a characteristic "ladder like" strip.
It can be seen that sodium fluoride can induce apoptosis of spermatogenic cells in mice, and zinc can play a protective role in this apoptosis.

【學(xué)位授予單位】：廣西大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類(lèi)號(hào)】：R363

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