本文選題：TCRαβT + 細(xì)胞�。� 參考：《江西醫(yī)學(xué)院》2005年碩士論文

【摘要】：目的了解TCRαβT 細(xì)胞和TCRγδT 細(xì)胞在正常大鼠小腸粘膜腸段間、上皮與固有層間的分布規(guī)律,探討它們?cè)跔C傷后的改變,以闡明燙傷后腸粘膜免疫功能的變化規(guī)律,為燙傷后腸源性感染的防治提供新的思路和形態(tài)學(xué)依據(jù)。 方法將40 只Wistar 雄性大鼠隨機(jī)分成4 組,即正常對(duì)照組、燙傷后1d 組、燙傷后3d 組和燙傷后7d 組,每組10 只。正常對(duì)照組,不給予蒸汽燙傷;另外三組用江西醫(yī)學(xué)院一附院燒傷實(shí)驗(yàn)中心研制的燙傷控制儀在背部脫毛區(qū)給予蒸汽燙傷,造成TBSA30%Ⅲo燙傷。經(jīng)抗休克處理后,分別于燙傷后1d(24h)、3d(72h)和7d 剖腹取十二指腸、空腸和回腸。恒冷切片機(jī)切片,對(duì)相鄰切片用小鼠抗大鼠CD_3單克隆抗體和小鼠抗大鼠TCRγδ單克隆抗體ABC免疫組織化學(xué)方法顯示CD_3~+T 細(xì)胞和TCRγδT 細(xì)胞,用方網(wǎng)測(cè)試系統(tǒng)測(cè)量和計(jì)數(shù),根據(jù)測(cè)量差值求出TCRαβT 細(xì)胞的數(shù)據(jù),分別求出其體密度、數(shù)密度、表面積密度、平均體積和表面積體積比等體視學(xué)參數(shù)。用統(tǒng)計(jì)軟件SPSS 10.0 for window 比較它們?cè)谀c段間、上皮與固有層間的差異。 結(jié)果(1)正常(未燙傷)大鼠十二指腸和空腸上皮內(nèi)TCRγδT 細(xì)胞數(shù)密度比TCRαβT 細(xì)胞大,而回腸上皮內(nèi)TCRγδT 細(xì)胞數(shù)密度比TCRαβT 細(xì)胞小。從十二指腸到回腸,上皮內(nèi)CD_3~+T 細(xì)胞和TCRγδT 細(xì)胞數(shù)密度依次遞減,而TCRαβT 細(xì)胞數(shù)密度遞增。(2)各腸段固有層TCRαβT 細(xì)胞數(shù)密度比TCRγδT 細(xì)胞大。從十二指腸到回腸,固有層中CD_3~+T 細(xì)胞和TCRαβT 細(xì)胞的數(shù)密度依次遞增,而TCRγδT 細(xì)胞的數(shù)密度變化不大。(3)十二指腸和空腸上皮內(nèi)CD3+T 細(xì)胞和TCRγδT 細(xì)胞數(shù)密度比固有層中的大;而回腸上皮內(nèi)CD3+T 細(xì)胞和TCRγδT 細(xì)胞數(shù)密度比固有層中的小。各腸段上皮內(nèi)TCRαβT 細(xì)胞數(shù)密度比固有層中的小。(4)各腸段粘膜中TCRγδT 細(xì)胞平均體積比TCRαβT 細(xì)胞的大。上皮內(nèi)T 細(xì)胞的平均體積比固有層T 細(xì)胞的大。各腸段間無(wú)差異。(5)上皮內(nèi)T 細(xì)胞表面積體積比值比固有層T 細(xì)胞的大,各腸段間無(wú)差異。TCRαβT 細(xì)胞與TCRγδT 細(xì)胞間也無(wú)差異。(6)燙傷后各腸段上皮和固有層CD3+T
[Abstract]:Objective to investigate the distribution of TCR 偽 尾 T cells and TCR 緯 未 T cells in the intestinal segments of the small intestine and between the epithelium and lamina propria of normal rats, and to explore their changes after scalding in order to elucidate the changes of intestinal mucosal immune function after scalding. To provide new ideas and morphological basis for the prevention and treatment of intestinal infection after scalding. Methods Forty male Wistar rats were randomly divided into 4 groups: normal control group, 1 day group, 3 d group and 7 d group, 10 rats in each group. In the control group, steam scalding was not given, and the other three groups were scalded with the scalding apparatus developed by the Burn Experimental Center of the first affiliated Hospital of Jiangxi Medical College, which caused TBSA 30% 鈪，

本文編號(hào)：1837584