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  本文選題：表皮細(xì)胞分離液 + 篩網(wǎng)過濾��； 參考：《鄭州大學(xué)》2006年碩士論文

【摘要】：背景 表皮細(xì)胞培養(yǎng)用于探討表皮生物學(xué)特性以及多種皮膚病的發(fā)病機(jī)理，也可用于皮膚藥理學(xué)，在燒傷外科及整形外科中亦有著廣闊的前景。人類進(jìn)行表皮細(xì)胞體外培養(yǎng)已有100余年的歷史，，很長時間內(nèi)不能解決人表皮細(xì)胞的體外長期大量培養(yǎng)問題，直到1975年Rheinwald和Green利用放射致死的3T3鼠成纖維細(xì)胞作為表皮細(xì)胞培養(yǎng)的滋養(yǎng)層細(xì)胞，才使表皮細(xì)胞的體外長期培養(yǎng)擴(kuò)增成為可能。隨著現(xiàn)代分子生物學(xué)和組織工程學(xué)的迅猛發(fā)展，以及研究手段和實(shí)驗(yàn)條件的進(jìn)一步改善，表皮角質(zhì)形成細(xì)胞培養(yǎng)在理論和技術(shù)上都有了不斷的發(fā)展和完善。 關(guān)于表皮細(xì)胞體外培養(yǎng)的適宜方法和條件，國內(nèi)外已有不少報道。然而，關(guān)于表皮細(xì)胞的分離液成分方面對表皮的克隆形成率，融合天數(shù)等細(xì)胞生物學(xué)性質(zhì)的影響卻缺乏深入的對比研究。為建立適宜的穩(wěn)定培養(yǎng)條件，包括滋養(yǎng)層問題，培養(yǎng)液內(nèi)容問題，尤其是關(guān)于表皮細(xì)胞分離液成分等問題，我們對正常人表皮生物工程中的適宜條件進(jìn)行了探討。此外，為了獲得更多的表皮深層細(xì)胞，我們還對分離表皮后的表皮細(xì)胞通過不同孔徑的尼龍布紗網(wǎng)過濾，對比不同的尼龍布紗網(wǎng)濾過的表皮細(xì)胞中深層細(xì)胞(主要指表皮干細(xì)胞)與中、淺層細(xì)胞的比例，為有關(guān)表皮生物學(xué)進(jìn)一步提供理論資料并為表皮生物工程研究奠定實(shí)驗(yàn)基礎(chǔ)。 材料與方法 標(biāo)本均取自我院2005年5月-2005年8月泌尿外科門診及住院包皮過長患者4例。年齡24-35歲。經(jīng)各項(xiàng)檢查證實(shí)無心、肝、肺、內(nèi)分泌、神經(jīng)系統(tǒng)疾病及腫瘤。以NIH-3T3細(xì)胞作為滋養(yǎng)層，以熱溶素分離表皮與真皮，分離表皮細(xì)胞應(yīng)用
[Abstract]:Background The culture of epidermal cells can be used to study the biological characteristics of epidermis and the pathogenesis of various skin diseases. It can also be used in dermatopharmacology and has a broad prospect in burn surgery and plastic surgery. Human epidermal cells have been cultured in vitro for more than 100 years, which can not solve the problem of human epidermal cells culture in vitro for a long time. It was not until 1975 that Rheinwald and Green used lethal 3T3 fibroblasts as trophoblast cells cultured in epidermal cells, which made it possible to expand epidermal cells in vitro for a long time. With the rapid development of modern molecular biology and tissue engineering, as well as the further improvement of research methods and experimental conditions, epidermal keratinocyte culture has been continuously developed and improved in theory and technology. There have been many reports on the suitable methods and conditions of epidermal cell culture in vitro. However, there is a lack of in-depth comparative study on the effects of the components of the separation fluid of epidermal cells on the cell biological properties such as the formation rate of epidermis, the days of fusion and so on. In order to establish suitable stable culture conditions, including trophoblast problem, culture medium content, especially the composition of epidermal cell isolate, we discussed the suitable conditions in normal human epidermis bioengineering. In addition, in order to obtain more deep epidermal cells, we also filter the epidermal cells after the separation of the epidermis through nylon gauze mesh with different pore sizes. The ratio of the deep cells (mainly epidermal stem cells) to the superficial cells (mainly epidermal stem cells) in the epidermal cells filtered by different nylon gauze mesh was compared, which provided further theoretical data for epidermal biology and laid an experimental foundation for the study of epidermal bioengineering. Materials and methods The specimens were collected from 4 outpatients of urology outpatient and inpatients in our hospital from May 2005 to August 2005. They are 24-35 years old. Unintentional, liver, lung, endocrine, nervous system diseases and tumors were confirmed by various examinations. NIH-3T3 cells were used as trophoblast, epidermis and dermis were separated by thermolysin, and epidermal cells were separated.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R329.2

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