本文選題：成纖維細(xì)胞 + 人工關(guān)節(jié)置換�。� 參考：《上海交通大學(xué)》2007年碩士論文

【摘要】： 【目的】研究應(yīng)用假膜成纖維細(xì)胞構(gòu)建人工關(guān)節(jié)周圍骨溶解裸鼠體內(nèi)模型的可行性。 【方法】本實驗于2006年5月至2007年1月于上海市第六人民醫(yī)院中心實驗室和動物實驗室完成,取裸鼠(SCID小鼠)15只,隨機(jī)分為三組,每組5只;分別為假膜成纖維細(xì)胞組(組I),關(guān)節(jié)囊成纖維細(xì)胞組(組II),對照組(組III),假膜成纖維細(xì)胞組從人工關(guān)節(jié)無菌性松動翻修術(shù)中取得的假膜組織,分離出成纖維細(xì)胞,經(jīng)過3～4次傳代后,應(yīng)用骨水泥刺激成纖維細(xì)胞后植入裸鼠的顱蓋骨上;關(guān)節(jié)囊成纖維細(xì)胞組從初次人工關(guān)節(jié)置換術(shù)中取出部分關(guān)節(jié)囊組織,分離出成纖維細(xì)胞經(jīng)過與組I同樣的處理后植入裸鼠的顱蓋骨上,對照組為假手術(shù)組;兩周后處死裸鼠,取出顱蓋骨脫鈣處理后切片,應(yīng)用影像軟件分析裸小鼠顱中縫的骨溶解情況,定量分析骨溶解的面積以判斷骨吸收的程度。 【結(jié)果】假膜組織和關(guān)節(jié)囊組織中分離出的細(xì)胞經(jīng)過鑒定表達(dá)成纖維細(xì)胞相關(guān)抗原(脯氨酸-4-羥化酶),經(jīng)RT-PCR檢測發(fā)現(xiàn)假膜成纖維細(xì)胞高表達(dá)RANKL與OPG的mRNA,假膜組織的成纖維細(xì)胞植入裸鼠顱蓋骨后可以從H-E染色的切片中觀察到裸鼠顱蓋骨骨吸收,經(jīng)過IPP軟件分析測量后假膜成纖維細(xì)胞組平均骨吸收面積為0.23±0.030 mm2(P0.05),明顯高于關(guān)節(jié)囊成纖維細(xì)胞組(組Ⅱ)(0.083±0.013mm2)和對照組(組Ⅲ)(0.078±0.009 mm2)。 【結(jié)論】從假膜中分離成纖維細(xì)胞可操作性強(qiáng),假膜成纖維細(xì)胞植入裸鼠后可以成功誘導(dǎo)骨溶解形成,可重復(fù)性強(qiáng),可以作為研究人工關(guān)節(jié)周圍骨溶解的動物模型。
[Abstract]:[objective] to study the feasibility of pseudomembrane fibroblast to construct osteolysis model of artificial joint in nude mice. [methods] from May 2006 to January 2007, the experiment was carried out in the Central Laboratory and Animal Laboratory of Shanghai sixth people's Hospital. Fifteen SCID mice were randomly divided into three groups with 5 mice in each group. The pseudomembrane fibroblasts group (group I), the joint capsule fibroblast group (group II), the control group (group II), and the pseudomembranous fibroblast group (group II) were isolated from the pseudomembranous tissue obtained from the artificial joint aseptic loosening revision. After 34 passages, bone cement was used to stimulate fibroblasts and implanted into the skull of nude mice. After the same treatment as group I, the fibroblasts were implanted into the skull of nude mice, the control group was sham-operated group, the nude mice were killed two weeks later, the skull cap was decalcified and then sliced. The osteolysis of cranial suture in nude mice was analyzed by image software, and the area of osteolysis was quantitatively analyzed to judge the degree of bone resorption. [results] the cells isolated from pseudomembranous tissue and articular capsule were identified to express fibroblast-associated antigen (proline -4-hydroxylase). By RT-PCR detection, it was found that pseudofibroblasts expressed RANKL and OPG mRNAs and pseudomembrane tissues. After implantation of fibroblasts into the skull of nude mice, bone resorption of skull cap of nude mice could be observed from H-E stained sections. The average bone resorption area of the pseudomembranous fibroblasts group was 0.23 鹵0.030 mm ~ (-2) (P 0.05), which was significantly higher than that of the articular capsule fibroblast group (group 鈪，

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