本文選題：立體定向 + 伏隔核��； 參考：《青島大學(xué)》2007年碩士論文

【摘要】： 目的：研究多靶點聯(lián)合毀損邊緣多巴胺系統(tǒng)對大鼠認(rèn)知功能的影響，從而為立體定向手術(shù)篩選出安全的手術(shù)靶點。 方法：選用成年Spraqui-Dawlay大鼠50只，其中40只作為毀損組，5只作為對照組，5只作為假手術(shù)組。毀損組又分為伏隔核毀損組(毀損部位：扣帶回+伏隔核)、杏仁核毀損組(毀損部位：扣帶回+杏仁核基底外側(cè)核)、海馬毀損組(毀損部位：扣帶回+海馬CA3區(qū))、隔區(qū)毀損組(毀損部位：扣帶回+隔區(qū))，每組10只。應(yīng)用直流電極立體定向技術(shù)同期對稱毀損大鼠不同靶點，利用組織學(xué)方法確定毀損部位及范圍，術(shù)后第1周、2周進(jìn)行利用Morris水迷宮定位航行試驗和空間探索試驗評估手術(shù)對大鼠認(rèn)知功能的影響。 結(jié)果：在成功準(zhǔn)確毀損邊緣多巴胺系統(tǒng)的靶點后，Morris水迷宮結(jié)果顯示：伏隔核毀損組術(shù)后第1周大鼠尋找平臺的逃避潛伏期延長，由10.1±1.2s延長至13.2±1.4s，具有明顯統(tǒng)計學(xué)差別：(t=16.24，P＜0.01)；第2周潛伏期縮短為9.6±0.9s，潛伏期短于術(shù)前水平，差別具有顯著性：(t=5.31，P＜0.01)。伏隔核組術(shù)后第1周在平臺象限內(nèi)停留的時間由術(shù)前41.5±3.3s縮短為術(shù)后35.1±2.8s，差別具有顯著性：(t=4.30，P=0.004)；術(shù)后第2周則恢復(fù)到術(shù)前水平。綜上所述，伏隔核組大鼠術(shù)后認(rèn)知功能有短暫的下降，但很快恢復(fù)至正�；蚋哂谡Ｋ�。杏仁核組結(jié)果分析表明與伏隔核組相似，，術(shù)后有短暫的下降，很快恢復(fù)正常。海馬毀損組則顯示術(shù)后逃避潛伏期明顯延長，探索時問明顯縮短，都具有統(tǒng)計學(xué)差異，并且在術(shù)后第2周無明顯變化。隔區(qū)毀損與海馬相似，但潛伏期延長和探索時間縮短程度都小于海馬組。 結(jié)論：1、杏仁核毀損對大鼠認(rèn)知功能無明顯影響；伏隔核毀損在術(shù)后可以出現(xiàn)短暫的認(rèn)知功能下降，但很快恢復(fù)至術(shù)前水平；海馬和隔區(qū)毀損大鼠可以出現(xiàn)認(rèn)知功能的近期和遠(yuǎn)期下降(至少是2周內(nèi))。對認(rèn)知功能的影響而言，依據(jù)作用大小依次排列為：海馬＞隔區(qū)＞伏隔核、杏仁核。2、杏仁核毀損是相關(guān)立體定向手術(shù)的安全、有效的靶點；伏隔核組毀損則可作為相關(guān)手術(shù)的備用靶點；而海馬和隔區(qū)毀損可嚴(yán)重影響大鼠認(rèn)知功能，不能作為手術(shù)靶點。
[Abstract]:Aim: to study the effects of multiple targets combined with lesion of edge dopamine system on cognitive function in rats, and to screen safe targets for stereotactic surgery. Methods: 50 adult Spraqui-Dawlay rats were selected, of which 40 were used as lesion group (n = 5) and 5 as control group (n = 5) as sham operation group. The lesion group was divided into two groups: nucleus accumbens lesion group (lesion site: cingulate nucleus accumbens nucleus), amygdaloid nucleus lesion group (lesion site: basal lateral nucleus of cingulate gyrus amygdala nucleus), hippocampal lesion group (lesion site: cingulate cortex hippocampal CA3 region). The septal lesion group (lesion site: cingulate gyrus septum, 10 rats in each group). The stereotactic technique of DC electrode was used to determine the location and range of damage by histological method. The effects of the operation on the cognitive function of rats were evaluated by Morris water maze navigation test and space exploration test 2 weeks after operation. Results: the results of Morris water maze showed that the escape latency of the rats in the nucleus accumbens group was prolonged at the first week after the successful and accurate destruction of the limbic dopamine system. From 10.1 鹵1.2 s to 13.2 鹵1.4 s, there was a significant difference between the two groups (P < 0.01), and the second week latency was shortened to 9.6 鹵0.9 s, which was shorter than the preoperative level (P < 0.01). In the nucleus accumbens group, the time of staying in the platform quadrant was shortened from 41.5 鹵3.3 s to 35.1 鹵2.8 s in the first week after operation. The difference was significant (P < 0.05), and recovered to the preoperative level at the second week after operation. In conclusion, the cognitive function of rats in nucleus accumbens group decreased briefly after operation, but quickly returned to normal or higher than normal level. The results of amygdaloid nucleus group showed that similar to the nucleus accumbens group, there was a brief decrease after operation and quickly returned to normal. In the hippocampal lesion group, the escape latency was prolonged and the time of exploration was shortened significantly, and there was no significant change in the second week after operation. The septal lesion was similar to that of hippocampus, but the prolongation of latency and the shortening of exploration time were smaller than those of hippocampal group. Conclusion the damage of amygdaloid nucleus has no obvious effect on cognitive function of rats, and the lesion of nucleus accumbens can cause a brief decline of cognitive function after operation, but it can recover to the level of preoperation. Cognitive impairment in the hippocampus and septum may occur in the near and long term (at least within 2 weeks). According to the effect of cognitive function, the order of action is: hippocampus > septum > nucleus accumbens, amygdala. The lesion of amygdala is a safe and effective target for stereotactic surgery. The lesion of nucleus accumbens can be used as a reserve target for related surgery, while the lesion of hippocampus and septum can seriously affect the cognitive function of rats and can not be used as a target for surgery.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R33

【參考文獻(xiàn)】

相關(guān)期刊論文 前3條

1 盧洪煊，柴東喬，田菊霞;杏仁核三維空間形態(tài)與位置的研究[J];立體定向和功能性神經(jīng)外科雜志;1996年04期

2 鐘明奎,何延龍,張景行,朱國慶,趙樂章;基底外側(cè)杏仁核中5-羥色胺抗抑郁作用的研究[J];中國行為醫(yī)學(xué)科學(xué);2001年04期

3 鐘明奎,朱國慶,張景行,章功良,張瑾,趙樂章;基底外側(cè)杏仁核內(nèi)一氧化氮對大鼠睡眠及行為活動的影響[J];中國行為醫(yī)學(xué)科學(xué);2002年03期

本文編號：1815351