本文選題：益生菌 + 鼠李糖乳桿菌�。� 參考：《揚(yáng)州大學(xué)》2006年碩士論文

【摘要】： 益生菌在促進(jìn)人體健康和防止疾病發(fā)生中發(fā)揮著重要的作用。本論文從大量分離獲得的乳酸菌菌株中,篩選具有優(yōu)良特性的益生菌,并對(duì)其進(jìn)行鑒定,為研究和開發(fā)具有自主知識(shí)產(chǎn)權(quán)的益生菌產(chǎn)品奠定基礎(chǔ)。 本論文從三個(gè)方面開展研究:即乳酸菌功能特性研究,以篩選獲得性能良好的益生菌;對(duì)篩選獲得的益生菌進(jìn)行鑒定;益生菌加工特性研究。 對(duì)分離的乳酸菌進(jìn)行耐酸和耐膽汁酸鹽試驗(yàn),供試的15個(gè)菌株都能在pH3、0.3%膽汁酸鹽中存活;供試菌株中有10個(gè)能在pH2、0.3%膽汁酸鹽中存活5h,其中Q菌的生長(zhǎng)情況最好,其耐受能力最強(qiáng);在pH3、0.2%膽汁酸鹽下只有Q菌能繼續(xù)生長(zhǎng)。 對(duì)分離的乳酸菌進(jìn)行了抑制致病性大腸桿菌和幽門螺旋桿菌的試驗(yàn),通過(guò)研究供試菌株對(duì)大腸桿菌均有抑制作用,抑制作用最強(qiáng)的為Q菌,抑菌圈直徑12.6mm,超過(guò)對(duì)照菌株BB-12的8mm。供試菌株大部分對(duì)幽門螺旋桿菌有抑制作用,抑制作用最強(qiáng)的為B菌,抑菌圈直徑13mm,其次為Q菌12.7mm,對(duì)照菌株BB-12為9.8mm。 對(duì)分離的乳酸菌進(jìn)行了體外粘附能力試驗(yàn),通過(guò)研究,供試菌株中對(duì)Caco-2細(xì)胞粘附能力最高的為A菌,其粘附平均值為18.7±5.92個(gè)/細(xì)胞,其次為Q菌12.9±2.74個(gè)/細(xì)胞,對(duì)照菌株BB-12為10.7±2.46個(gè)/細(xì)胞。先用乳酸菌粘附2h后,Q菌和E菌對(duì)大腸桿菌競(jìng)爭(zhēng)粘附抑制力最強(qiáng);先用大腸桿菌粘附2h,再用乳酸菌粘附,粘附數(shù)與空白大腸桿菌粘附數(shù)相差不大。 對(duì)分離的乳酸菌進(jìn)行了抗氧化試驗(yàn),通過(guò)研究Q菌受試品喂服小鼠8天使得SOD活力增強(qiáng),MDA含量有所降低,具有一定的抗氧化能力。 在功能特性研究的基礎(chǔ)上,對(duì)篩選獲得的Q菌,進(jìn)行初步鑒定。經(jīng)傳統(tǒng)的形態(tài)學(xué)觀察及代謝產(chǎn)物氣相色譜測(cè)定分析,Q菌歸屬于乳桿菌屬(Lactobacillus)。通過(guò)API 50CHL試驗(yàn)條生理生化特性研究,鑒定Q菌99.9%為鼠李糖乳桿菌(Lactobacillus rhamnosus)。該菌株已在中國(guó)微生物菌種保藏管理委員會(huì)普通微生物中心保藏,保藏號(hào)為CGMCC No.1531,中國(guó)專利申請(qǐng)?zhí)枮?200510111588.9,菌株名為鼠李糖乳桿菌LV108。 在此基礎(chǔ)上,進(jìn)一步利用分子生物學(xué)方法對(duì)鼠李糖乳桿菌LV108進(jìn)行了16S rRNA基因的擴(kuò)增與測(cè)序,BLAST在線比對(duì)結(jié)果是:與菌株Lactobacillus rhamnosus MCRF-412和Lactobacillus rhamnosus MCRF-271 16S rRNA的基因序列只相差一個(gè)堿基,同源性高達(dá)99.9%。與全球最著名的益生菌Lactobacillus rhamnosus GG ATCC 53103的16S rRNA只相
[Abstract]:Probiotics play an important role in promoting human health and preventing disease. In this paper, probiotics with excellent characteristics were screened from a large number of isolated strains of lactic acid bacteria, which laid a foundation for the research and development of probiotic products with independent intellectual property rights. In this paper, three aspects of research are carried out: the study on the functional characteristics of lactic acid bacteria in order to select probiotics with good performance; the identification of the probiotics obtained by screening; and the study on the processing characteristics of probiotics. The acid tolerance and bile salt tolerance tests of the isolated lactic acid bacteria showed that all of the 15 strains were able to survive in pH3.3% bile salt, and 10 of the tested strains could survive in pH2.3% bile salt for 5 h, among which the growth of Q bacteria was the best. Its tolerance ability was strongest, and only Q bacteria could continue to grow at pH 3. 2% bile acid salt. The experiment of inhibiting pathogenic Escherichia coli and Helicobacter pylori was carried out on the isolated lactic acid bacteria. By studying the inhibitory effect of the tested strains on Escherichia coli, Q bacteria was the most effective. The diameter of inhibition circle was 12.6 mm, which was higher than that of the control strain BB-12 (8 mm). Most of the tested strains had inhibitory effect on Helicobacter pylori, the strongest inhibition was B, the diameter of inhibition circle was 13mm, the second was Q (12.7mm), the control strain BB-12 was 9.8 mm. The adhesion ability of isolated lactic acid bacteria to Caco-2 cells was tested in vitro. The results showed that A strain had the highest adhesion ability to Caco-2 cells, with an average adhesion of 18.7 鹵5.92 cells per cell, followed by Q bacteria 12.9 鹵2.74 cells per cell. The BB-12 of the control strain was 10.7 鹵2.46 / cell. Lactic acid bacteria were used to adhere to E. coli for 2 hours, and then to E. coli for 2 hours, and then to lactic acid bacteria for 2 hours, the adhesion number was not different from that of blank Escherichia coli. The antioxidation test of isolated lactic acid bacteria was carried out. The SOD activity increased and the content of MDA decreased in mice fed with Q bacteria for 8 days. Based on the study of functional characteristics, the screening of Q bacteria was preliminarily identified. By traditional morphological observation and gas chromatographic analysis of metabolites, the bacteria belong to Lactobacillus. By studying the physiological and biochemical characteristics of the API 50CHL test strip, 99.9% of the bacteria were identified as Lactobacillus rhamnosus. The strain has been stored in the General microorganism Center of the Chinese microbial species Conservation Administration Committee under the name of CGMCC No. 1531, and the patent application number of China is 1: 200510111588.9. the strain is called Lactobacillus rhamnoides LV108. On this basis, the 16s rRNA gene of Lactobacillus rhamnoides LV108 was amplified by molecular biology and sequenced by blast online. The results showed that the gene sequence of Lactobacillus rhamnoides LV108 was only one base different from that of Lactobacillus rhamnosus MCRF-412 and Lactobacillus rhamnosus MCRF-271 16S rRNA. The homology is as high as 99.9. The 16s rRNA of Lactobacillus rhamnosus GG ATCC 53103, the most famous probiotic in the world
【學(xué)位授予單位】：揚(yáng)州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R371

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 趙紅霞;;不同益生菌在奶粉中的穩(wěn)定性研究[J];乳業(yè)科學(xué)與技術(shù);2011年02期

相關(guān)碩士學(xué)位論文 前2條

1 李巖巖;鼠李糖乳桿菌與干酪乳桿菌純種發(fā)酵豆乳產(chǎn)品貯藏穩(wěn)定性及菌體腸道耐受性研究[D];河北農(nóng)業(yè)大學(xué);2012年

2 孟令頻;原料乳中干酪非發(fā)酵劑乳酸菌的分離和鑒定[D];北京林業(yè)大學(xué);2008年

，

本文編號(hào)：1778845