本文選題：催產(chǎn)素 + 促性腺激素釋放激素��； 參考：《西北農(nóng)林科技大學(xué)》2007年碩士論文

【摘要】： 為了探討催產(chǎn)素(OT)對(duì)促性腺激素釋放激素(GnRH)的調(diào)節(jié)作用及其作用機(jī)制,本研究通過腹腔注射OT,采用免疫組化法觀察OT對(duì)成年小鼠下丘腦-垂體-性腺軸中GnRH表達(dá)的影響;另外,為了研究OT對(duì)哺乳動(dòng)物后代性比的調(diào)控作用,本研究通過腹腔注射和陰道注射OT觀察OT對(duì)成年小鼠后代性別比例的影響。 結(jié)果顯示:給成年雄性小鼠腹腔注射OT,GnRH廣泛分布于下丘腦-垂體-睪丸軸中,下丘腦GnRH的表達(dá)量與對(duì)照組顯著降低,下降幅度與OT劑量呈劑-效關(guān)系。腺垂體GnRH的表達(dá)量與對(duì)照組相比都有降低,且OT低劑量組有極顯著性差異;神經(jīng)垂體中GnRH免疫陽性產(chǎn)物未見表達(dá)。結(jié)果表明OT對(duì)下丘腦-垂體系統(tǒng)GnRH表現(xiàn)抑制作用。GnRH免疫陽性產(chǎn)物主要分布于睪丸間質(zhì)細(xì)胞和曲細(xì)精管的生精細(xì)胞和支持細(xì)胞,在被膜中未見陽性產(chǎn)物表達(dá);睪丸間質(zhì)GnRH的表達(dá)量與對(duì)照組相比都有升高,且OT低劑量組有極顯著性差異;睪丸曲細(xì)精管GnRH的表達(dá)量隨OT劑量的增加呈下降趨勢(shì),其中OT高劑量組與對(duì)照組相比有極顯著性差異。結(jié)果表明OT對(duì)睪丸間質(zhì)GnRH表現(xiàn)促進(jìn)作用,而對(duì)曲細(xì)精管GnRH表現(xiàn)抑制作用。 給發(fā)情前期成年雌性小鼠腹腔注射OT,GnRH廣泛分布于下丘腦-垂體-卵巢軸中,下丘腦GnRH的表達(dá)量與對(duì)照組相比顯著增加。腺垂體GnRH的表達(dá)量與對(duì)照組相比都有增加,且OT低劑量組有顯著性差異;神經(jīng)垂體中GnRH免疫陽性產(chǎn)物未見表達(dá)。結(jié)果表明OT對(duì)下丘腦-垂體系統(tǒng)GnRH表現(xiàn)促進(jìn)作用。在卵巢的被膜、皮質(zhì)和髓質(zhì)未見陽性產(chǎn)物表達(dá),而在黃體細(xì)胞和卵泡顆粒細(xì)胞中觀察到有GnRH免疫陽性產(chǎn)物的存在;卵巢黃體和卵泡中GnRH表達(dá)量與對(duì)照組相比都有下降,其中OT低劑量組下降幅度大于OT高劑量組。結(jié)果表明OT對(duì)卵巢GnRH表現(xiàn)抑制作用。 成年小鼠按雄給藥組、雌給藥組、雌雄給藥組分別腹腔注射OT后,合籠交配,結(jié)果顯示:雌給藥組的不同劑量組的雌/雄性別比例都高于對(duì)照組,且高劑量組與對(duì)照組相比差異極顯著。不同劑量組的雌/雄性別比例在雄給藥組和雌雄給藥組的變化趨勢(shì)不定。結(jié)果提示外源性O(shè)T促進(jìn)小鼠后代性別偏雌性,其影響依賴于給藥劑量和給藥方式。對(duì)成年雌性小鼠陰道注射OT,結(jié)果顯示各劑量組的雌/雄性別比例都高于對(duì)照組,而且與劑量有正相關(guān)關(guān)系。結(jié)果表明子代小鼠雌/雄性比隨OT劑量增加有上升趨勢(shì)。提示OT作用于雌鼠有助于生偏雌性的后代。
[Abstract]:In order to investigate the effect and mechanism of oxytocin (OT) on gonadotropin releasing hormone (GnRH), the effect of OT on the expression of GnRH in the hypothalamus pituitary - gonadal axis of adult mice was observed by intraperitoneal injection of OT. In addition, in order to study the regulation effect of OT on the sex ratio of mammalian offspring, this study was conducted through this study. Intraperitoneal injection and vaginal injection of OT were used to observe the effect of OT on the sex ratio of offspring in adult mice.
The results showed that OT was injected into the abdominal cavity of adult male mice, and GnRH was widely distributed in the hypothalamus pituitary testicular axis. The expression of GnRH in the hypothalamus was significantly lower than that of the control group. The decrease was in the dose effect relationship with the dose of OT. The expression of GnRH in the adeno pituitary was lower than that of the control group, and there was a significant difference in the OT low dose group; G in the neurohypophysis was G. The results showed that the immune positive products of nRH were not expressed. The results showed that OT had inhibitory effect on GnRH in the hypothalamus pituitary system. The positive products of.GnRH were mainly distributed in spermatogenic cells and support cells of Leydig cells and tubuloseminiferous tubules, and no positive products were expressed in the membrane; the expression of GnRH in testis was higher than that of the control group, and OT There was a very significant difference in the low dose group; the expression of GnRH in the testicular seminiferous tubule decreased with the increase of the dose of OT, and there was a significant difference between the high dose group of OT and the control group. The results showed that OT had a promoting effect on the GnRH expression of the testis and the inhibitory effect on the GnRH of the seminiferous tubule.
OT was intraperitoneally injected into the pre estrus adult female mice, and GnRH was widely distributed in the hypothalamus pituitary ovary axis. The expression of GnRH in the hypothalamus increased significantly compared with the control group. The expression of GnRH in the adenohypophysis was increased compared with the control group, and there was a significant difference in the OT low dose group; the GnRH positive products in the neurohypophysis had not been expressed. The results showed that OT could promote the GnRH performance of the hypothalamus pituitary system. There was no expression of positive products in the membrane, cortex and medulla of the ovary, but the presence of GnRH immunoreactive products in the corpus luteum and follicle granulosa cells, and the GnRH expression in the corpus luteum and follicle of the ovary decreased as compared with those in the group, and the low dose group of OT decreased. The amplitude was greater than that of OT high dose group. The results showed that OT inhibited ovarian GnRH expression.
In the adult mice, the female group, the female and the male group, and the male and female administration group were intraperitoneally injected with OT to mate with the cage. The results showed that the female / male ratio of the female to the different dose groups was higher than that of the control group, and the high dose group was significantly different from the control group. The female / male ratio in the different dose groups was in the male and the male and male groups. The results suggested that exogenous OT promoted the female offspring of female offspring, and the effect depended on the dosage and the way of administration. The vaginal injection of OT to adult female mice showed that the female / male ratio of each dose group was higher than that of the control group, and the relationship with the dose was positive. The results showed that the female / male ratio of the mice was with OT. The dose increase has an upward trend, suggesting that OT acting on female mice is beneficial to the offspring of female offspring.

【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：R33

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