本文選題：免疫傳感器 + 電化學(xué)傳感器　； 參考：《浙江大學(xué)》2006年碩士論文

【摘要】：本課題的研究成功地實(shí)現(xiàn)了電化學(xué)免疫陣列傳感器快速檢測(cè)腸出血性大腸桿菌O157:H7。利用巰基物質(zhì)在金表面的自組裝特性,在金陣列上形成穩(wěn)定、有序、緊密的,尾基為羧基的16-巰基十六烷酸(MHDA)自組裝膜(Self-assembled Monolayers,SAMs);通過(guò)1-乙基-3-(3-二甲基氨丙酸)碳二亞胺/N-羥基琥珀酰亞胺(EDC/NHS)的活化作用在電極表面形成活性酯中間體,使抗體更有效的固定到SAMs上;因抗原抗體的特異性結(jié)合,將大腸桿菌O157:H7捕獲到電極上,進(jìn)行電化學(xué)測(cè)量,從而實(shí)現(xiàn)了對(duì)細(xì)菌的定量檢測(cè)。 在存在鐵氰化鉀/亞鐵氰化鉀氧化還原對(duì)的緩沖溶液中,測(cè)量電極各步處理結(jié)果的循環(huán)伏安曲線和電化學(xué)阻抗譜。循環(huán)伏安曲線可用來(lái)定性的分析電極表面的修飾狀況以及修飾層的穩(wěn)定性。根據(jù)電化學(xué)阻抗譜良好的界面表征特性,選取合適的等效電路對(duì)阻抗譜進(jìn)行分析。相對(duì)常相位角元件,電子轉(zhuǎn)移電阻的改變最明顯,增幅最大。從而可以確定,免疫傳感器的修飾以及大腸桿菌的固定主要影響了電子轉(zhuǎn)移電阻。通過(guò)分析電子轉(zhuǎn)移電阻在不同濃度菌液中響應(yīng)的變化,實(shí)現(xiàn)對(duì)濃度的定量分析。檢測(cè)過(guò)程中金電極陣列的使用,給多樣本檢測(cè)節(jié)省了時(shí)間。該免疫傳感器的檢測(cè)下限可達(dá)到2.0×10~2 CFU/ml,檢測(cè)線性范圍為2.0×10~2～2.0×10~7 CFU/ml。單個(gè)樣本的檢測(cè)時(shí)間僅需65分鐘。
[Abstract]:In this paper, electrochemical immunosensor was successfully used to detect EHEC O 157: h 7. Based on the self-assembly properties of thiol compounds on gold surface, a stable, ordered, compact, carboxyl Cetadecanoic acid (MHDA) self-assembled self-assembled film was formed on gold arrays. MonolayersThe active ester intermediates were formed on the surface of the electrode by the activation of 1-ethyl-3-trimethylpropionic acid) carbodiimide / N-hydroxy-succinimide (EDCN / NHS) on the surface of the electrode, which made the antibody more effectively immobilized on the SAMs; because of the specific binding of the antigen and antibody, The Escherichia coli O157:H7 was captured on the electrode for electrochemical measurement, thus the quantitative detection of bacteria was realized. The cyclic voltammetry curves and electrochemical impedance spectra of the electrode were measured in the buffer solution with redox pairs of potassium ferricyanide / potassium ferricyanide. Cyclic voltammetry curves can be used to qualitatively analyze the surface modification and the stability of the modified layer. According to the good interface characterization characteristics of electrochemical impedance spectroscopy, the appropriate equivalent circuit is selected to analyze the impedance spectrum. Compared with the constant phase angle element, the change of the electron transfer resistance is the most obvious and the increase is the largest. It can be concluded that the modification of the immunosensor and the fixation of Escherichia coli mainly affect the electron transfer resistance. The quantitative analysis of electron transfer resistance was realized by analyzing the response of electron transfer resistance in different concentrations of bacteria. The use of gold electrode array in the detection process saves time for multi-sample detection. The detection limit of the immunosensor can reach 2.0 脳 10 ~ (-2) CFU / ml, and the linear range is 2.0 脳 10 ~ (2) ~ (2) 脳 10 ~ (7) CFU / ml. The detection time for a single sample is only 65 minutes.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R318.6;R378

【引證文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 王麗江;結(jié)合納米技術(shù)的細(xì)菌檢測(cè)分子生物傳感器的研究[D];浙江大學(xué);2007年

，

本文編號(hào)：1775904