本文選題：小鼠胃腸道 + 小鼠胰腺�。� 參考：《山西醫(yī)科大學(xué)》2007年碩士論文

【摘要】： 目的:觀察發(fā)育中小鼠胃腸道和胰腺內(nèi)膽囊收縮素免疫反應(yīng)細(xì)胞(cholecystokininimmunoreactive cells,CCK-IR細(xì)胞)和胰多肽免疫反應(yīng)細(xì)胞(pancreatic polypeplideimmunoreactive cells,PP-IR細(xì)胞)的發(fā)生、分布、形態(tài)和數(shù)量變化。 方法:應(yīng)用免疫組織化學(xué)SP法觀察小鼠胚胎第12天至出生后45天胃腸道和胰腺內(nèi)CCK-IR細(xì)胞和PP-IR細(xì)胞的發(fā)生、分布及形態(tài)特征。 結(jié)果:小鼠胚胎發(fā)育第16天小腸內(nèi)出現(xiàn)CCK-IR細(xì)胞,第19天,結(jié)腸內(nèi)出現(xiàn)CCK-IR細(xì)胞。小鼠的胃體部?jī)H在胚胎第18、19天偶見CCK-IR細(xì)胞,生后未見CCK-IR細(xì)胞;而胃竇部始終未見CCK-IR陽性細(xì)胞。小鼠胰腺中的CCK-IR細(xì)胞,最早出現(xiàn)于胚胎第12天,出生后直至成年,胰腺中均可見到CCK-IR細(xì)胞。 胚胎第16-19天小鼠的小腸絨毛上皮內(nèi)可見散在的CCK-IR細(xì)胞;出生后,CCK-IR細(xì)胞多位于絨毛上皮和固有層的腸腺中。結(jié)腸的CCK-IR細(xì)胞分布在黏膜上皮和腸腺中。胰腺中的CCK-IR細(xì)胞主要分布在胰島,染色深淺不一;外分泌部腺泡和導(dǎo)管的上皮細(xì)胞之間,可見散在、深染的CCK-IR細(xì)胞。 統(tǒng)計(jì)結(jié)果表明,小腸:從胚胎期直至出生后發(fā)育成熟,CCK-IR細(xì)胞數(shù)量持續(xù)增多,出生后30天數(shù)量達(dá)高峰。其中,出生后1天、7天和15天組,回腸CCK-IR細(xì)胞的數(shù)量較十二指腸和空腸多。45天組,小腸的CCK-IR細(xì)胞數(shù)量顯著減少。結(jié)腸:胚胎期偶見CCK-IR細(xì)胞。生后1-30天,CCK-IR細(xì)胞數(shù)量隨發(fā)育增加,生后45天組,數(shù)量減少。胰腺:胚胎期CCK-IR細(xì)胞的數(shù)量以第15天最多,之后逐漸減少,生后胰腺內(nèi)僅偶見CCK-IR細(xì)胞。 小鼠胰腺中的PP-IR細(xì)胞最早見于胚胎第17天,結(jié)腸中的PP-IR細(xì)胞最早出現(xiàn)于胚胎第18天,出生后直至成年,胰腺和結(jié)腸中均可見PP-IR細(xì)胞。發(fā)育中小鼠的胃體、胃竇和小腸各段始終未見PP-IR細(xì)胞。 發(fā)育中小鼠胰腺內(nèi)PP-IR細(xì)胞主要分布于胰島的周邊部,外分泌部腺泡和導(dǎo)管的上皮內(nèi),可見散在的PP-IR細(xì)胞。統(tǒng)計(jì)結(jié)果表明,胰腺中PP-IR細(xì)胞從胚胎第17天至生后第15天,數(shù)量逐漸增多,并于第15天組數(shù)量達(dá)高峰。生后30天組數(shù)量減少,45天組數(shù)量再次增多。胚胎第18天到發(fā)育成熟小鼠結(jié)腸黏膜上皮和腸腺中始終可見PP-IR細(xì)胞,生后30天組,PP-IR細(xì)胞的數(shù)量較其它時(shí)段多。 小鼠腸道和胰腺內(nèi)CCK-IR細(xì)胞和PP-IR細(xì)胞的形態(tài)多樣,可見免疫反應(yīng)陽性細(xì)胞的突起伸至其它細(xì)胞之間。胰腺內(nèi)的CCK-IR細(xì)胞有強(qiáng)弱兩種陽性表達(dá)方式。 結(jié)論:從胚胎第16天至出生后30天,小鼠小腸與結(jié)腸內(nèi)的CCK-IR細(xì)胞數(shù)量持續(xù)增加,其分泌的CCK可能促進(jìn)小腸和結(jié)腸的生長(zhǎng)發(fā)育。發(fā)育中小鼠胰腺內(nèi)CCK-IR細(xì)胞數(shù)量的變化提示,胰腺內(nèi)表達(dá)的CCK其主要作用是刺激胰腺的生長(zhǎng)、分化和發(fā)育。 發(fā)育中小鼠胰腺和胰腺外器官中PP-IR細(xì)胞的分布,與人和其它種屬動(dòng)物間存在明顯差異;胰腺和結(jié)腸中PP-IR細(xì)胞的數(shù)量變化提示,胰多肽可能參與調(diào)控胰腺和結(jié)腸的功能活動(dòng)。 CCK-IR細(xì)胞和PP-IR細(xì)胞均可見免疫反應(yīng)陽性細(xì)胞的突起伸至其它細(xì)胞之間,細(xì)胞外還可見免疫反應(yīng)陽性物質(zhì)逸出,這提示,兩種內(nèi)分泌細(xì)胞都可能以旁分泌方式影響周圍細(xì)胞的功能活動(dòng)。
[Abstract]:Objective: To observe the development of cholecystokinin immunoreactive cells in the gastrointestinal tract and pancreas in mice (cholecystokininimmunoreactive cells, CCK-IR cells) and pancreatic polypeptide immunoreactive cells (pancreatic polypeplideimmunoreactive cells, PP-IR cells) occurrence, distribution, morphology and number of changes.
Methods: immunohistochemical SP method was used to observe the occurrence, distribution and morphological characteristics of CCK-IR cells and PP-IR cells in the gastrointestinal tract and pancreas during twelfth days to 45 days after birth.
Results: the mouse embryos of CCK-IR cells in the small intestine, sixteenth days and nineteenth days, CCK-IR cells in the colon. The body of the stomach of mice at embryonic day 18,19 only a few CCK-IR cells, no CCK-IR cells after birth; and the gastric antrum had no CCK-IR positive cells in the mouse pancreas. CCK-IR cells first appeared on embryonic day twelfth after birth until adulthood, the pancreas can be seen in CCK-IR cells.
Embryonic day 16-19 mouse intestinal villi epithelium scattered within CCK-IR cells; after birth, CCK-IR cells located in the villous epithelium and lamina propria of the intestinal glands. Colon CCK-IR cells distributed in mucosa and intestinal gland. CCK-IR cells in the pancreas are mainly distributed in the islet, dyeing depth between the exocrine portion; acinar and ductal epithelial cells, scattered, deep staining of CCK-IR cells.
The statistical results showed that the small intestine: from the embryonic period until postnatal maturation, CCK-IR cell number continued to increase, 30 days after birth number reached the highest peak. Among them, 1 days after birth, 7 days and 15 days group, the number of ileum in CCK-IR cells compared with the duodenum and jejunum.45 day group, the number of CCK-IR cells was significantly reduced in small intestine I see. Colon: embryonic CCK-IR cells. 1-30 days after birth, the number of CCK-IR cells increased with the development, 45 days after birth. The decrease in the number of groups, the number of embryos: pancreatic CCK-IR cells for up to fifteenth days, then decreased gradually after birth, only occasionally seen in pancreatic CCK-IR cells.
The PP-IR cells in the pancreas of the mice were first seen on the seventeenth day of the embryo. The PP-IR cells in the colon first appeared on the eighteenth day of the embryo. After birth, they could see PP-IR cells in the pancreas and colon. There were no PP-IR cells in the gastric body, the antrum and the small intestine in developing mice.
The development of PP-IR cells and pancreas was mainly distributed in the periphery of the islets and exocrine acinar and ductal epithelial, scattered in PP-IR cells. The statistical results showed that the PP-IR cells in the pancreas from embryonic day seventeenth to fifteenth days after birth, the number gradually increased, reached the peak at fifteenth days after birth. The number of groups and 30 to reduce the number of days group, 45 days group increased again. The number of embryonic day eighteenth to always visible PP-IR cells mature mouse colon mucosa and intestinal gland in 30 days after birth, the number of PP-IR cells than the other periods.
The morphology of CCK-IR cells and PP-IR cells in the intestine and pancreas of mice is diverse. It is found that the protrusion of immunoreactive cells extends to other cells. There are two positive expressions of CCK-IR in pancreas.
Conclusion: from the 30 day embryo sixteenth days after birth, small intestine and colon of mice CCK-IR cells continued to increase, the secretion of CCK may promote the growth and development of small intestine and colon. Developmental changes in the number of CCK-IR cells in the pancreas and pancreas suggest that the expression of CCK and its main role is to stimulate pancreatic growth and differentiation development.
The distribution of PP-IR cells in the pancreas and extra pancreatic organs of developing rats is significantly different from that of humans and other species. The number of PP-IR cells in the pancreas and colon suggests that pancreatic polypeptide may participate in regulating the function of pancreas and colon.
Both CCK-IR cells and PP-IR cells showed the protrusion of immunoreactive cells extended to other cells, and the presence of immunoreactive substances also appeared outside the cell. This suggests that all two endocrine cells may influence the function of peripheral cells in paracrine manner.

【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類號(hào)】：R329

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