本文選題：食管癌 + 動(dòng)物模型��； 參考：《湖南農(nóng)業(yè)大學(xué)》2007年碩士論文

【摘要】： 目的建立人食管癌免疫SCID小鼠異位移植模型并對(duì)其轉(zhuǎn)移性淋巴來(lái)源細(xì)胞特性進(jìn)行初級(jí)研究。方法將食管癌細(xì)胞系Eca-109細(xì)胞懸液注射至SCID小鼠胃壁。三個(gè)月后或動(dòng)物頻死時(shí)處死解剖,觀察腫瘤在小鼠體內(nèi)的生長(zhǎng)轉(zhuǎn)移情況,將肉眼可見(jiàn)的縱膈淋巴結(jié)轉(zhuǎn)移瘤塊接種于SCID鼠皮下擴(kuò)增,然后取小鼠皮下瘤組織進(jìn)行細(xì)胞培養(yǎng),得到穩(wěn)定的細(xì)胞株后用MTT法分析細(xì)胞的生長(zhǎng)曲線(xiàn),Western bloting法檢測(cè)拓?fù)洚悩?gòu)酶的表達(dá),酶譜法檢測(cè)MMP-2,MMP-9的活性,劃痕實(shí)驗(yàn)和Transwell體外移動(dòng)實(shí)驗(yàn)檢測(cè)細(xì)胞的移動(dòng)能力。結(jié)果成功建立裸鼠胃壁篩選第一代高轉(zhuǎn)移潛力細(xì)胞,并命名為NMC109,對(duì)其生物學(xué)特征研究發(fā)現(xiàn)其細(xì)胞增殖能力,移動(dòng)能力和侵襲潛力都發(fā)生明顯增強(qiáng)。結(jié)論:腫瘤胃壁移植是建立食管癌免疫缺陷動(dòng)物自發(fā)性移植模型和篩選轉(zhuǎn)移性細(xì)胞亞系的可行方法。
[Abstract]:Objective to establish the heterotopic transplantation model of human esophageal carcinoma immunized SCID mice and to study the characteristics of metastatic lymphoid cells.Methods the suspension of esophageal carcinoma cell line Eca-109 was injected into the gastric wall of SCID mice.After three months or when the animal died frequently, the tumor was killed and the tumor growth and metastasis were observed in mice. The mediastinal lymph node metastases were inoculated into SCID mice subcutaneously, and then the subcutaneous tumor tissues were taken from the mice for cell culture.After the stable cell line was obtained, the expression of topoisomerase was detected by Western bloting, the activity of MMP-2 and MMP-9 was detected by MTT assay, and the mobility of cells was detected by scratch test and Transwell in vitro mobility assay.Results the first generation of high metastatic potential cells were successfully established in nude mice gastric wall and named NMC109. The biological characteristics of NMC109 showed that the cell proliferation, mobility and invasion potential of NMC109 cells were significantly enhanced.Conclusion: tumor gastric wall transplantation is a feasible method for the establishment of spontaneous transplantation model and screening of metastatic cell sublines in immunodeficient esophageal carcinoma.
【學(xué)位授予單位】：湖南農(nóng)業(yè)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類(lèi)號(hào)】：R-332;R735.1

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