發(fā)布時間：2018-04-12 10:55

  本文選題：單核巨噬細胞 + 絡(luò)風(fēng)寧號��； 參考：《中西醫(yī)結(jié)合心腦血管病雜志》2014年10期

【摘要】：目的制備單核巨噬細胞炎癥模型,并觀察絡(luò)風(fēng)寧1號含藥血清對模型細胞增殖的影響。方法采用脂多糖(LPS)體外誘導(dǎo)建立單核巨噬細胞炎癥模型,造模的同時加入不同濃度的絡(luò)風(fēng)寧1號及匹伐他汀鈣含藥血清干預(yù),共同孵育24 h后,收集細胞上清,ELISA法檢測炎癥因子基質(zhì)金屬蛋白酶9(MMP 9)水平。結(jié)果與空白組相比,模型組THP 1單核細胞顯著升高(P0.01);不同濃度絡(luò)風(fēng)寧1號含藥血清干預(yù)后,高濃度組的抑制作用最明顯(P0.01)。結(jié)論單核巨噬細胞炎癥模型制備成功,基于此模型,絡(luò)風(fēng)寧1號含藥血清可以抑制LPS對炎癥細胞的增殖作用,這可能是其抗炎癥、防治冠心病不穩(wěn)定性心絞痛的主要機制之一。
[Abstract]:Objective to establish a mononuclear macrophage inflammatory model and to observe the effect of Luofuning 1 serum on the proliferation of the model cells.Methods the mononuclear macrophage inflammatory model was induced by lipopolysaccharide (LPS) in vitro. The model was incubated with different concentrations of Luofenin 1 and pivastatin calcium containing serum for 24 h.The level of inflammatory factor matrix metalloproteinase (9(MMP 9) was detected by Elisa.Results compared with the blank group, the monocyte of THP 1 in the model group was significantly higher than that in the control group, and the inhibitory effect of the high concentration group was the most obvious after the intervention of different concentrations of Luofunning 1 containing serum.Conclusion the mononuclear macrophage inflammatory model was successfully prepared. Based on this model, the drug serum Luofuning 1 can inhibit the proliferation of LPS on inflammatory cells, which may be one of the main mechanisms of anti-inflammation and preventing and treating unstable angina pectoris of coronary heart disease.
【作者單位】： 中國中醫(yī)科學(xué)院廣安門醫(yī)院;北京中醫(yī)藥大學(xué)東直門醫(yī)院;
【分類號】：R329.2;R285.5

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本文編號：1739435