發(fā)布時(shí)間：2018-04-11 14:47

  本文選題：年齡 + 軟骨細(xì)胞　； 參考：《山西醫(yī)科大學(xué)》2007年碩士論文

【摘要】： 目的:對不同年齡的兔關(guān)節(jié)軟骨細(xì)胞的黏彈性進(jìn)行定量的檢測,以從細(xì)胞水平闡明關(guān)節(jié)軟骨的退變過程,了解生物力學(xué)因素在骨性關(guān)節(jié)炎發(fā)病機(jī)理中的作用。為軟骨組織工程中相關(guān)力學(xué)因素的研究提供新思路和新方法,為軟骨組織工程種子細(xì)胞時(shí)機(jī)的選擇提供理論依據(jù)。 方法:15只雌性新西蘭白兔,按照年齡分為3組:幼年組5只(約6周齡)、成年組5只(約7月齡)、老年組5只(約31月齡)。各組實(shí)驗(yàn)動(dòng)物均用空氣栓塞處死,取左側(cè)膝關(guān)節(jié)標(biāo)本做大體觀察,同時(shí)進(jìn)行石蠟切片HE染色觀察關(guān)節(jié)軟骨隨年齡增長的組織學(xué)變化;利用透射電鏡觀察隨年齡增長關(guān)節(jié)軟骨細(xì)胞超微結(jié)構(gòu)的變化;利用激光共聚焦掃描顯微鏡(laser confocal scanning microscopeLCSM)對軟骨細(xì)胞直徑進(jìn)行檢測;利用微管吸吮技術(shù)(micropipette aspiration)結(jié)合半無限體細(xì)胞力學(xué)模型(Half-space model)對右側(cè)膝關(guān)節(jié)所分離的軟骨細(xì)胞進(jìn)行黏彈性的檢測。 結(jié)果:不同年齡兔關(guān)節(jié)軟骨的大體觀察及HE染色觀察都有明顯的變化,即關(guān)節(jié)軟骨隨著年齡的增長將會發(fā)生退行性改變;透射電鏡觀察發(fā)現(xiàn)老年組軟骨細(xì)胞的超微結(jié)構(gòu)發(fā)生了明顯的變化;幼年軟骨細(xì)胞的直徑為8.84±0.97μm、成年軟骨細(xì)胞的直徑為9.77±1.41μm、老年軟骨細(xì)胞的直徑為11.50±1.76μm,統(tǒng)計(jì)分析表明三者兩兩之間的差異有統(tǒng)計(jì)學(xué)意義(p＜0.001)。關(guān)節(jié)軟骨細(xì)胞表現(xiàn)為典型的黏彈性固體蠕變特性,隨著年齡的增長,其黏彈性參數(shù)(瞬時(shí)模量E_0、平衡模量E_∞、表觀黏性μ)逐漸降低,幼年組E_0(中位數(shù)±四分位數(shù)間距)為0.68±0.15kaPa、E_∞為0.38±0.16kPa、μ為6.42±1.36 kPa·s;成年組E_0為0.63±0.10kPa、E_∞為0.33±0.06kPa、μ為5.84±1.35 kPa·s;老年組E_0為0.56±0.08kPa、E_∞為0.28±0.05kPa、μ為4.06+0.71kPa·s。統(tǒng)計(jì)學(xué)分析表明幼年組及中年組的黏彈性特性均與老年組的差異有統(tǒng)計(jì)學(xué)意義(p＜0.005),而幼年組與中年組的黏彈性特性比較無統(tǒng)計(jì)學(xué)意義(p＞0.1)。 結(jié)論:不同年齡的關(guān)節(jié)軟骨細(xì)胞都表現(xiàn)出典型的黏彈性固體蠕變特征;不同年齡關(guān)節(jié)軟骨細(xì)胞的直徑有明顯的差別;老年關(guān)節(jié)軟骨細(xì)胞的黏彈性特性較幼年及成年軟骨細(xì)胞明顯下降。
[Abstract]:Aim: to quantitatively detect the viscoelasticity of articular chondrocytes in rabbits at different ages in order to elucidate the degeneration process of articular cartilage at cellular level and understand the role of biomechanical factors in the pathogenesis of osteoarthritis.It provides a new idea and method for the study of related mechanical factors in cartilage tissue engineering, and provides a theoretical basis for the timing of seed cells in cartilage tissue engineering.Methods Fifteen female New Zealand white rabbits were divided into three groups according to their age: 5 young rabbits (about 6 weeks old), 5 adult rabbits (about 7 months old) and 5 elderly rabbits (31 months old).All the experimental animals were killed by air embolization. The specimens of the left knee joint were taken for gross observation, and the histological changes of articular cartilage with age were observed by HE staining in paraffin sections.The ultrastructural changes of articular chondrocytes were observed with transmission electron microscope (TEM), and the diameter of chondrocytes was detected by laser confocal scanning microscopeLCSM (laser confocal scanning microscope).Micropipette aspiration and Half-space model were used to detect the viscoelasticity of chondrocytes isolated from the right knee joint.Results: the gross observation and HE staining of articular cartilage in rabbits of different ages showed obvious changes, that is, the articular cartilage would degenerate with the increase of age.The ultrastructure of chondrocytes in the aged group was obviously changed by transmission electron microscope.The diameter of juvenile chondrocytes was 8.84 鹵0.97 渭 m, that of adult chondrocytes was 9.77 鹵1.41 渭 m, and that of senile chondrocytes was 11.50 鹵1.76 渭 m.Articular chondrocytes showed typical viscoelastic solid creep properties. With age, the viscoelastic parameters (instantaneous modulus E _ 0, equilibrium modulus E _ 鈭，

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