本文選題：超廣譜酶β-內(nèi)酰胺酶　切入點(diǎn)：大腸埃希氏菌　出處：《大連醫(yī)科大學(xué)》2006年碩士論文

【摘要】： 細(xì)菌中可移動(dòng)遺傳單位如:質(zhì)粒、轉(zhuǎn)座子和整合子可以攜帶編碼耐藥基因的基因盒。整合子是耐藥基因水平轉(zhuǎn)移的主要因子。整合子通過(guò)位點(diǎn)特異的基因重組在臨床細(xì)菌耐藥性傳播中起重要作用。根據(jù)整合酶基因intI的序列不同將整合子劃分成四種類型,其中第一類整合子最常見(jiàn),決定著細(xì)菌的多重耐藥。目前已在革蘭氏陰性菌中發(fā)現(xiàn)插入整合子的許多耐藥基因盒,在I類整合子中有50多種不同的基因結(jié)構(gòu),可編碼60多個(gè)基因盒,介導(dǎo)對(duì)氨基糖苷類、青霉素類、頭孢菌素類、碳青霉烯類、利福平、磺胺增效劑、氯霉素、紅霉素和四價(jià)銨化合物等的耐藥。產(chǎn)ESBLs菌株在整合子水平轉(zhuǎn)移的介導(dǎo)下能增加捕獲這些基因盒的可能性,更有利于耐藥基因的傳遞和擴(kuò)散。 目的:了解I類整合子在產(chǎn)ESBLs和非產(chǎn)ESBLs以及健康人群大腸埃希氏菌中分布流行狀況,分析I類整合子在細(xì)菌多重耐藥中的作用。 方法:用VITEK-AMS微生物自動(dòng)分析儀鑒定細(xì)菌和藥敏試驗(yàn),用雙紙片協(xié)同試驗(yàn)檢測(cè)產(chǎn)ESBLs菌。用PCR方法擴(kuò)增I類整合酶基因,經(jīng)電泳后檢測(cè)擴(kuò)增產(chǎn)物。用χ2檢驗(yàn)進(jìn)行統(tǒng)計(jì)學(xué)分析,P0.05被認(rèn)為有差異。 結(jié)果: 140株大腸埃希氏菌檢出I類整合子48株,檢出率為34.3%。I類整合子在產(chǎn)ESBLs菌(c-ESBLs)的分布明顯高于非產(chǎn)ESBLs菌(c-nESBLs)和健康人群(h-n ESBLs ),檢
[Abstract]:Mobile genetic units in bacteria such as plasmids transposons and integrons can carry boxes of genes that encode drug-resistant genes.Integron is the main factor of drug resistance gene level transfer.Integron plays an important role in the transmission of drug resistance of clinical bacteria through locus-specific gene recombination.Integrons are divided into four types according to the sequence of integrase gene intI. The first type of integron is the most common, which determines the multidrug resistance of bacteria.At present, many drug-resistant gene cassettes inserted integron have been found in Gram-negative bacteria. There are more than 50 different gene structures in class I integron, which can encode more than 60 gene cassettes, mediating p-aminoglycoside, penicillin, cephalosporins.Carbapenem, rifampicin, sulfonamide synergist, chloramphenicol, erythromycin and tetravalent ammonium compounds resistance.The possibility of capturing these gene cassettes can be increased by ESBLs producing strain mediated by horizontal transfer of integron, which is more favorable to the transmission and diffusion of drug resistance genes.Objective: to investigate the distribution and prevalence of class I integron in ESBLs producing, non producing ESBLs and healthy individuals, and to analyze the role of class I integron in multidrug resistance of bacteria.Methods: VITEK-AMS microorganism automatic analyzer was used to identify bacteria and drug sensitivity test, and double disk synergy test was used to detect ESBLs producing bacteria.Class I integrase gene was amplified by PCR, and the products were detected by electrophoresis.蠂 2 test was used for statistical analysis. P0.05 was considered to be different.Results: 48 strains of class I integron were detected from 140 strains of Escherichia coli. The positive rate of class I integron in ESBLs producing strain c-ESBLswas significantly higher than that of non-#en1# producing strain c-nESBLs) and healthy population.
【學(xué)位授予單位】：大連醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R378

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本文編號(hào)：1726564