本文關(guān)鍵詞： miR-a iASPP 神經(jīng)發(fā)育 突起生長　出處：《南方醫(yī)科大學(xué)學(xué)報(bào)》2014年01期 　論文類型：期刊論文

【摘要】：目的探索miR-124a的靶基因iASPP對神經(jīng)發(fā)育的影響。方法利用在線預(yù)測軟件TargetScan對miR-124a的靶基因進(jìn)行預(yù)測,結(jié)合文獻(xiàn)資料提到的與神經(jīng)早期發(fā)育有關(guān)的基因進(jìn)行篩選,我們選擇iASPP作為miR-124a的候選靶基因。通過熒光素酶報(bào)告基因?qū)嶒?yàn)對其進(jìn)行驗(yàn)證,然后在M17細(xì)胞中轉(zhuǎn)染miR-124a過表達(dá)質(zhì)粒,用Western blotting實(shí)驗(yàn)檢測iASPP蛋白表達(dá)水平的變化。利用視黃酸誘導(dǎo)M17細(xì)胞作為神經(jīng)元分化模型,通過過表達(dá)或者基因干擾的方法,初步探討iASPP基因?qū)ι窠?jīng)發(fā)育的影響。結(jié)果 miR-124a促進(jìn)神經(jīng)突起發(fā)育,并且能夠與iASPP基因的3'非翻譯區(qū)(3'UTR)相互作用來抑制其蛋白質(zhì)的表達(dá);iASPP基因的過表達(dá)抑制神經(jīng)突起的發(fā)育并且抑制miR-124a促進(jìn)神經(jīng)突起生長的作用。結(jié)論 miR-124a能夠通過抑制iASPP基因的表達(dá)來促進(jìn)神經(jīng)突起生長。
[Abstract]:Objective to explore the effect of target gene iASPP of miR-124a on neural development. Methods the target gene of miR-124a was predicted by online prediction software TargetScan. We selected iASPP as the candidate target gene for miR-124a, verified it by luciferase reporter gene experiment, then transfected miR-124a overexpression plasmid in M17 cells. The expression of iASPP protein was detected by Western blotting assay. M17 cells were induced by retinoic acid as a neuronal differentiation model. The effect of iASPP gene on nerve development was studied. Results miR-124a promoted neurite development. And it can interact with the 3'untranslated region of iASPP gene to inhibit its protein expression. The overexpression of IASPP gene can inhibit the development of neurite and inhibit the effect of miR-124a on the growth of neurite. Conclusion miR-124a can promote the growth of neurite by using miR-124a. Inhibit the expression of iASPP gene to promote neurite growth.
【作者單位】： 南方醫(yī)科大學(xué)基礎(chǔ)醫(yī)學(xué)院神經(jīng)生物學(xué)教研室;
【基金】：國家自然科學(xué)基金(81071016)~~
【分類號】：R363

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