本文關(guān)鍵詞： mecA基因 金黃色葡萄球菌 耐甲氧西林金黃色葡萄球菌 耐藥性　出處：《青島大學》2005年碩士論文　論文類型：學位論文

【摘要】：目的 探討mecA基因在金黃色葡萄球菌(Staphylococcus aureus,S.aureus)中的分布以及mecA基因?qū)瘘S色葡萄球菌耐藥性的影響。 方法 收集金黃色葡萄球菌臨床分離菌株47例,選取12種針對葡萄球菌最常用的抗菌藥物(苯唑青霉素、青霉素、氨芐西林、哌拉西林、阿莫西林棒酸、頭孢噻吩、頭孢噻肟、頭孢吡肟、左旋氧氟沙星、丁胺卡那霉素、替考拉寧以及萬古霉素),用瓊脂擴散法(改良Kirby-Bauer法)進行敏感性檢測。提取并純化47例臨床金黃色葡萄球菌分離株DNA,通過聚合酶鏈反應(PCR)對mecA基因進行擴增,分析mecA基因和金黃色葡萄球菌耐藥性的關(guān)系,同時結(jié)合病人臨床資料進行綜合分析。 結(jié)果 ①47株金黃色葡萄球菌中,有33株為耐甲氧西林的金黃色葡萄球菌(MRSA),占70.2%;甲氧西林敏感型金黃色葡萄球菌(MSSA)有14株,占29.8%。在33株MRSA中,只有3株對除替考拉寧、萬古霉素等糖肽類以外的抗菌藥物敏感,占9.1%;14株MSSA中只有2株對全部12種抗菌藥物敏感,僅占14.3%。②PCR結(jié)果顯示,在33株MRSA中,32株攜帶mecA基因,占97.0%,只有1株不攜帶mecA基因。而在14株MSSA中有3株攜帶mecA基因,3株攜帶mecA基因的MSSA耐藥性較不攜帶mecA基因MSSA的耐藥性嚴重。③47例金黃色葡萄球菌感染中45例屬于醫(yī)院感染,其中15例屬于暴發(fā)感染。 結(jié)論 ①金黃色葡萄球菌中耐甲氧西林菌株(MRSA)具有較高的分離率。②MRSA的耐藥性非常嚴重,萬古霉素等糖肽類抗生素是唯一有效的抗菌藥物。MSSA的耐藥性也較嚴重。③絕大多數(shù)的MRSA攜帶有mecA基因,mecA基因在金黃色葡萄球菌的耐藥機制中發(fā)揮重要作用。有少數(shù)MSSA攜帶有mecA基因。攜帶mecA基因的MSSA耐藥情況比不攜帶mecA基因的MSSA嚴重。④本地區(qū)金黃色葡萄球菌的感染仍以醫(yī)院感染為主;在醫(yī)院內(nèi)容易發(fā)生金黃色葡萄球菌感染的暴發(fā)。
[Abstract]:Objective to investigate the distribution of mecA gene in Staphylococcus aureus (S.aureus) and the effect of mecA gene on the resistance of Staphylococcus aureus.
Methods the clinical isolates of Staphylococcus aureus in 47 cases, selected 12 kinds of antibacterial drugs for Staphylococcus aureus is the most commonly used (oxacillin, penicillin, ampicillin, piperacillin, amoxicillin clavulanic acid, cephalothin, cefotaxime, cefepime, levofloxacin, amikacin and teicoplanin. Vancomycin), using agar diffusion method (modified Kirby-Bauer method) were extracted and purified. The sensitivity of detection of 47 clinical isolates of Staphylococcus aureus DNA by polymerase chain reaction (PCR) of mecA gene were amplified by analysis of the relationship between mecA gene and drug resistance of Staphylococcus aureus, and the clinical data of patients were analyzed.
Results 47 strains of Staphylococcus aureus, Staphylococcus aureus 33 strains of methicillin resistant (MRSA), accounting for 70.2%; methicillin sensitive Staphylococcus aureus (MSSA) accounted for 29.8%. in the 14 strains, 33 strains of MRSA, only 3 strains of teicoplanin, vancomycin outside sugar peptide antimicrobial susceptibility of 14 strains of MSSA, accounting for 9.1%; only 2 of all strains were sensitive to 12 kinds of antibiotics, accounting for only 14.3%. of PCR showed that the 33 strains MRSA, 32 strains carried mecA gene, accounted for 97%, only 1 strains carrying mecA gene in 14 strains of MSSA, 3 strains carrying mecA gene, 3 strains of resistance of MSSA carrying mecA gene carrying mecA gene MSSA is not serious. In 47 cases of Staphylococcus aureus infection in 45 cases of hospital infection, including 15 cases belonging to the outbreak of infection.
Methicillin resistant strains of Staphylococcus aureus (MRSA) separation rate is very high. The resistance of MRSA is very serious, glycopeptides antibiotics are only effective antimicrobial drug resistance of.MSSA is more serious. The vast majority of MRSA carrying mecA gene, mecA gene play an important role in drug resistance the mechanism of Staphylococcus aureus. There are a few MSSA carrying mecA gene. Carrying MSSA resistance gene mecA is not carrying mecA gene MSSA. The serious region of Staphylococcus aureus infection in hospital infection; susceptible to hospital outbreaks of Staphylococcus aureus infection.

【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2005
【分類號】：R378

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本文編號：1461375