本文關(guān)鍵詞： 間充質(zhì)干細胞 干細胞因子 粒細胞集落刺激因子 外周血 軟骨細胞　出處：《河北醫(yī)科大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

【摘要】： 背景: 間充質(zhì)干細胞(MSC)是指一群具有向成骨細胞、成軟骨細胞、成脂肪細胞、肌腱細胞甚至肝臟細胞、神經(jīng)細胞等多種細胞分化潛能的多能干細胞。由于其分化的多樣性及在體外易分離培養(yǎng)和擴增的特性使其成為組織工程學(xué)的理想種子細胞。在骨科領(lǐng)域具有廣泛的應(yīng)用前景,主要應(yīng)用于骨修復(fù)和骨形成、軟骨修復(fù)、肌腱和韌帶組織修復(fù)等。MSC主要存在于骨髓、脂肪組織、軟骨及骨組織、臍帶血、胎盤中,其中以骨髓中含量最為豐富。外周血中是否存在MSC仍有很大爭議。本試驗通過聯(lián)合應(yīng)用干細胞因子(SCF)和粒細胞集落刺激因子(G-CSF)動員小鼠外周血培養(yǎng)MSC,探索外周血MSC的培養(yǎng)方法和生物學(xué)特性。目的: 探討聯(lián)合利用SCF和G-CSF動員小鼠外周血培養(yǎng)MSC的分離、培養(yǎng)和擴增方法。并初步研究在體外單層培養(yǎng)條件下向軟骨細胞方向誘導(dǎo)的條件,為臨床上利用外周血來源的間充質(zhì)干細胞修復(fù)關(guān)節(jié)軟骨缺損提供前期的資料和方法。 方法: 將40只4-6周齡BALB/C小鼠隨機分為兩組。試驗組應(yīng)用SCF及G-CSF動員后的小鼠外周血,2只BALB/C小鼠外周血為一份,對照組應(yīng)用注射生理鹽水小鼠的外周血作
[Abstract]:Background: Mesenchymal stem cell (MSCs) is a group of osteoblasts, chondroblasts, adipoblasts, tendon cells and even liver cells. Pluripotent stem cells with differentiation potential such as nerve cells. Due to their diversity of differentiation and easy isolation, culture and expansion in vitro, they are ideal seed cells for tissue engineering. They are widely used in orthopaedics. The prospect of its application. MSC mainly exists in bone marrow, adipose tissue, cartilage and bone tissue, umbilical cord blood and placenta. The presence of MSC in peripheral blood is still controversial. In this study, the combination of stem cell factor (SCF) and granulocyte colony stimulating factor (G-CSF) was used. MSC was mobilized in peripheral blood of mice. Objective: to explore the culture methods and biological characteristics of peripheral blood MSC. To explore the method of isolation, culture and amplification of MSC from peripheral blood of mice mobilized by SCF and G-CSF, and to study the conditions of inducing MSC to chondrocytes under monolayer culture in vitro. To provide clinical data and methods for repairing articular cartilage defects with peripheral blood derived mesenchymal stem cells. Methods: Forty 4-6 week-old BALB/C mice were randomly divided into two groups. In the experimental group, the peripheral blood of 2 BALB/C mice mobilized by SCF and G-CSF was one. The control group was treated with peripheral blood of mice injected with normal saline.
【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2006
【分類號】：R329

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