本文關(guān)鍵詞： 骨髓間充質(zhì)干細(xì)胞 5-氮雜胞苷 血清 細(xì)胞分化　出處：《吉林大學(xué)》2006年博士論文　論文類型：學(xué)位論文

【摘要】：骨骼肌在嚴(yán)重?fù)p傷后,由于肌細(xì)胞不能再生,其修復(fù)方式主要通過瘢痕修復(fù),愈合后收縮功能差,骨髓間充質(zhì)干細(xì)胞(mesenchymal stem cells,MSCs)作為成體干細(xì)胞的一種,具備多項(xiàng)分化潛能,在肌組織創(chuàng)傷修復(fù)方面具有重要作用。如何將MSCs定向誘導(dǎo)分化為完美成熟的肌細(xì)胞是目前正在研究的問題。 我們在成功分離并培養(yǎng)MSCs后,分別通過5-氮雜胞苷及骨骼肌損傷動物模型的不同時期血清連續(xù)誘導(dǎo)兩種方法對MSCs進(jìn)行誘導(dǎo),并將誘導(dǎo)后的MSCs局部注射入損傷的肌組織中,觀察其在損傷重建中發(fā)揮的作用。結(jié)果表明兩種方法均能將MSCs誘導(dǎo)成肌細(xì)胞,而血清誘導(dǎo)組肌細(xì)胞發(fā)育更成熟。認(rèn)識到,5-Aza作為單一的化學(xué)信號與損傷動物血清的生理性調(diào)節(jié)信號相比較,在MSCs分化成骨骼肌細(xì)胞方面存在一定差距。MSCs最終要分化為成熟的目的細(xì)胞,其誘導(dǎo)方式是非常復(fù)雜的。同時證明了應(yīng)用肌肉損傷動物不同時期血清連續(xù)誘導(dǎo)MSCs向肌細(xì)胞分化成為可能,提出MSCs誘導(dǎo)分化的一個新途徑。
[Abstract]:After severe injury, skeletal muscle cells can not regenerate, its repair way is mainly through scar repair, the contraction function is poor after healing. As a kind of adult stem cells, bone marrow mesenchymal stem cells have multiple differentiation potential. It plays an important role in wound repair of muscle tissue. How to induce and differentiate MSCs into perfect mature myocytes is a problem that is currently being studied. After successfully isolating and culturing MSCs, MSCs was induced by 5-azacytidine and serum at different stages of skeletal muscle injury. The induced MSCs was injected into the injured muscle tissue to observe its role in the reconstruction of the injury. The results showed that both methods could induce MSCs into muscle cells. However, the development of muscle cells in serum-induced group was more mature. It was recognized that 5-Aza as a single chemical signal was compared with the physiological regulatory signal of injured animal serum. There is a certain gap in the differentiation of MSCs into skeletal muscle cells. It is proved that it is possible to continuously induce the differentiation of MSCs into myocytes by serum in different stages of muscle injury, and a new way of inducing differentiation by MSCs is put forward.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：博士
【學(xué)位授予年份】：2006
【分類號】：R329.2

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