本文關(guān)鍵詞： 抗癌中草藥 大黃素 芹菜素 電化學(xué) DNA　出處：《鄭州大學(xué)》2007年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】： 目前，中藥尤其抗癌中草藥引起了許多制藥機(jī)構(gòu)的高度重視，因?yàn)檠芯空唛_(kāi)始意識(shí)到中草藥對(duì)于藥物發(fā)展來(lái)說(shuō)是一個(gè)巨大的來(lái)源，，而且中藥的毒性比較低，很多沒(méi)有副作用。據(jù)知，現(xiàn)在對(duì)中草藥的研究多集中在提純、分離方面，在其性質(zhì)及其抗癌機(jī)理的研究仍然很欠缺。本論文研究了抗癌中草藥大黃素和芹菜素的電化學(xué)性質(zhì)，建立了兩種藥物的電化學(xué)分析方法，并運(yùn)用電化學(xué)和光譜學(xué)手段研究了抗癌中草藥大黃素和芹菜素與DNA的相互作用，初步討論了大黃素通過(guò)插入到DNA的雙螺旋鏈中來(lái)抑制DNA的合成，從而達(dá)到抗腫瘤的效果；芹菜素與DNA在該實(shí)驗(yàn)條件下不發(fā)生作用，顯示了芹菜素低毒的特性。本論文主要包括以下幾個(gè)內(nèi)容： 1．研究了抗癌藥物大黃素在玻碳電極上的電化學(xué)行為。在pH 7.2的NH_3-NH_4Cl(50％無(wú)水乙醇)緩沖溶液中，大黃素有三個(gè)均受吸附控制的氧化和還原峰，還原峰的峰電位為-0.688V(P1)，兩個(gè)氧化峰的峰電位為-0.628V(P2)和-0.235V(P3)。其中P3峰的峰電流與大黃素的濃度在8.9×10~(-8)M～7.8×10~(-6)M范圍內(nèi)有良好的線性關(guān)系，檢出限為7.8×10~(-9)M，對(duì)三黃片實(shí)際樣品中大黃素含量的測(cè)定，不用預(yù)分離其它蒽醌類(lèi)化合物，結(jié)果令人滿意。 2．利用多種電化學(xué)手段研究了抗癌中草藥芹菜素在B-R(50％無(wú)水乙醇，pH 9.0)緩沖底液中的電化學(xué)性質(zhì)。循環(huán)伏安圖譜表明其電極過(guò)程是一擴(kuò)散控制為主的氧化反應(yīng)，呈現(xiàn)了一個(gè)不可逆的氧化峰。文章分別運(yùn)用Laviron理論計(jì)算了芹菜素在玻碳電極上的動(dòng)力學(xué)參數(shù)，并以該氧化峰為研究對(duì)象建立了芹菜素的電化學(xué)分析方法。在所選實(shí)驗(yàn)條件下，芹菜素濃度在5.0×10~(-6)M～9.0×10~(-5)M范圍內(nèi)與峰電流存在良好的線性關(guān)系，檢測(cè)限為1.5×10~(-6)M，并對(duì)密蒙花實(shí)際樣品的芹菜素進(jìn)行了含量測(cè)定，結(jié)果令人滿意。并用差示脈沖伏安法和紫外可見(jiàn)方法研究了芹菜素與DNA的相互作用，結(jié)果表明在該實(shí)驗(yàn)條件下，芹菜素與DNA不發(fā)生相互作用，從而顯示了芹菜素低毒的一面。 3．在pH 5.5，0.05 mol／LNH_4Cl-HCl(50％無(wú)水乙醇)緩沖溶液中，利用差示脈沖技術(shù)在裸玻碳電極和DNA修飾電極上研究了抗癌中草藥大黃素與DNA的相互作用，結(jié)果表明隨著DNA的加入，大黃素的峰電位發(fā)生了明顯正移且峰電流降低，同時(shí)紫外可見(jiàn)光譜也表現(xiàn)出其440nm的吸怛光度值降低，最大吸收紅移，而且在400nm和475nm處出現(xiàn)兩個(gè)等吸收點(diǎn)，所有這些表明大黃素以其接近平面的結(jié)構(gòu)插入到DNA的雙螺旋結(jié)構(gòu)中，從而抑制DNA的合成，達(dá)到抗腫瘤效果。另外，在最優(yōu)化條件下，大黃素峰電流的降低與DNA的濃度在一定范圍內(nèi)存在良好的線性關(guān)系，以此進(jìn)行了DNA樣品回收率的測(cè)定，結(jié)果令人滿意。
[Abstract]:At present, Chinese medicine, especially anti-cancer Chinese medicine, has attracted great attention from many pharmaceutical organizations, because researchers have begun to realize that Chinese medicine is a huge source of drug development, and the toxicity of traditional Chinese medicine is relatively low. Many have no side effects. It is known that the current research on Chinese herbal medicine is focused on purification and separation. In this paper, the electrochemical properties of emodin and apigenin were studied, and the electrochemical analysis method of two kinds of drugs was established. The interaction of emodin and apigenin with DNA was studied by electrochemical and spectroscopic methods. The inhibition of DNA synthesis by emodin inserted into the double helix chain of DNA was discussed preliminarily. In order to achieve the effect of anti-tumor; Apigenin and DNA do not interact under this experimental condition, which shows the low toxicity of apigenin. This thesis mainly includes the following contents: 1. The electrochemical behavior of the anticancer drug emodin on glassy carbon electrode was studied in the pH 7.2 NH3-NH4Cl-50% anhydrous ethanol buffer solution. Emodin had three oxidation and reduction peaks controlled by adsorption, and the peak potential of the reduction peak was -0.688V ~ (-1). The peak potentials of the two oxidation peaks were -0.628 V ~ (2) and -0.235 V ~ (3) P _ (3). The peak current of P3 peak and the concentration of emodin were 8.9 脳 10 ~ (-8)). There is a good linear relationship in the range of 7.8 脳 10 ~ (-1) ~ (-6) ~ (-1). The detection limit of emodin in Sanhuang tablets was 7.8 脳 10 ~ (10) ~ (-9) 渭 m. The determination of emodin in Sanhuang tablets was satisfactory without the need of preseparation of other anthraquinones. 2. 50% anhydrous ethanol with apigenin, an anticancer herb, was studied by various electrochemical methods. The electrochemical properties in buffer solution were determined by pH 9.0. The cyclic voltammetry showed that the electrode process was mainly a diffusion-controlled oxidation reaction. There is an irreversible oxidation peak. The kinetic parameters of apigenin on glassy carbon electrode were calculated by Laviron theory. The electrochemical analysis method of apigenin was established based on the oxidation peak. There was a good linear relationship between the concentration of apigenin and peak current in the range of 5.0 脳 10 ~ (-1) -6 ~ (-1) M ~ (9) 脳 10 ~ (10) ~ (-5) ~ (5) M, and the detection limit was 1.5 脳 10 ~ (10) ~ (-6) 渭 m. The content of apigenin in the actual sample was determined with satisfactory results. The interaction between apigenin and DNA was studied by differential pulse voltammetry and UV-vis. The results showed that there was no interaction between apigenin and DNA under the experimental conditions, which showed the low toxicity of apigenin. 3. In the buffer solution of pH 5.5 ~ (0.05) mol / L ~ (NH _ 4NH _ 4Cl-HCln _ (50)% anhydrous alcohol). The interaction between emodin and DNA was studied on bare glassy carbon electrode and DNA modified electrode by differential pulse technique. The results showed that the interaction between emodin and DNA was increased with the addition of DNA. The peak potential of emodin showed a significant positive shift and peak current decreased, while the UV-Vis spectrum also showed a decrease in absorbance value of 440 nm, and the maximum absorption red shift. Moreover, there are two isoabsorption points at 400nm and 475nm, all of which indicate that emodin is inserted into the double helix structure of DNA with its near plane structure, thus inhibiting the synthesis of DNA. In addition, under the optimal conditions, there is a good linear relationship between the peak current of emodin and the concentration of DNA in a certain range, so the recovery rate of DNA samples is determined. The result is satisfactory.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2007
【分類(lèi)號(hào)】：R285;R284

【引證文獻(xiàn)】

相關(guān)博士學(xué)位論文 前1條

1 呂平;赭曲霉發(fā)酵產(chǎn)大黃素及其生物合成途徑和誘發(fā)機(jī)制的研究[D];天津大學(xué);2009年

本文編號(hào)：1450583