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  本文關(guān)鍵詞：模式識別技術(shù)在篩選溶脲脲原體和人精子交叉抗原中的應(yīng)用研究　出處：《上海交通大學(xué)》2007年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 機器學(xué)習(xí) 模式識別 支持向量機 主成分分析 線性判別分析 生物信息學(xué) BLAST 散列表 交叉抗原 B細胞表位 溶脲脲原體 人精子細胞

【摘要】： 免疫生物學(xué)研究表明,人精子細胞和溶脲脲原體(簡稱UU)存在著交叉反應(yīng)抗原(又稱交叉抗原)。篩選鑒定這些交叉抗原對闡述UU致男性不育機理以及研制避孕疫苗具有重要意義。傳統(tǒng)的篩選交叉抗原的方法大多基于生物學(xué)實驗,過程非常復(fù)雜,其中的蛋白質(zhì)提純是一個技術(shù)難點,有時候由于量少而無法提純,從而導(dǎo)致實驗無法繼續(xù)而失敗。生物學(xué)實驗篩選人精子和UU間的交叉抗原便存在著這樣的問題。當今,UU的基因組測序已經(jīng)完成,其基因組編碼的蛋白質(zhì)(包括實驗鑒定以及預(yù)測工具預(yù)測的蛋白質(zhì))均可以在蛋白質(zhì)數(shù)據(jù)庫中檢索到。另外,蛋白質(zhì)數(shù)據(jù)庫中還可以檢索到特異性表達在人精子細胞中的蛋白質(zhì)。利用這些數(shù)據(jù)集,根據(jù)交叉抗原的結(jié)構(gòu)序列特性,應(yīng)用模式識別的技術(shù)篩選潛在的交叉抗原是一個值得研究的課題,這也是論文研究的主要內(nèi)容。 發(fā)生免疫交叉反應(yīng)的抗原之間含有相同或者相似的B細胞表位。利用這一基本原理,論文設(shè)計了一種從UU和人精子蛋白質(zhì)數(shù)據(jù)集中篩選出候選交叉抗原及其B細胞表位的方法。首先,搜索SWISS-PROT數(shù)據(jù)庫,查找出所有UU蛋白質(zhì)和特異性表達在人精子中的蛋白質(zhì),建立相應(yīng)數(shù)據(jù)集;其次,在UU和人精子細胞蛋白質(zhì)數(shù)據(jù)集中查找出給定長度的相同或相似子序列;再者,對這些子序列進行B細胞表位的預(yù)測,選擇B細胞表位可能性大的子序列,這些子序列即候選的交叉反應(yīng)B細胞表位,其所在的抗原即候選的交叉抗原。這里需要解決兩個關(guān)鍵的問題:一是如何在兩個海量數(shù)據(jù)集中有效快速地查找給定長度的相同或者相似子序列;二是如何有效地進行B細胞表位的預(yù)測。論文對以上兩個問題
[Abstract]:Immunobiological studies have shown. Human sperm cells and Ureaplasma Urealyticum (UUU) have cross-reaction antigens (also known as cross-antigens). Screening and identification of these cross antigens is of great significance in explaining the mechanism of male sterility induced by UU and in developing contraceptive vaccine. Traditional methods for screening cross antigens are mostly based on biological experiments. The process is very complex, in which protein purification is a technical difficulty, sometimes due to a small amount of can not be purified. This led to the failure of the experiment. This is the problem of screening cross-antigens between human sperm and UU in biological experiments. Today, the genome sequencing of UU has been completed. The proteins encoded by its genome (including those identified by experiments and predicted by predictive tools) can be found in the protein database. Proteins specifically expressed in human sperm cells can also be found in the protein database. The application of pattern recognition in screening potential cross-antigens is a subject worthy of study, which is also the main content of this paper. The same or similar B cell epitopes are present between antigens that are immune cross-reactive. Use this basic principle. A method of screening candidate cross-antigens and their B cell epitopes from UU and human sperm protein data sets was designed. Firstly, the SWISS-PROT database was searched. All UU proteins and proteins specifically expressed in human spermatozoa were found and the corresponding data sets were established. Secondly, the same or similar subsequences of a given length were found in the UU and human sperm cell protein datasets. Furthermore, the B cell epitopes were predicted and the B cell epitopes were selected, which were the candidate cross reaction B cell epitopes. Two key problems need to be solved here: first, how to find the same or similar subsequences of a given length effectively and quickly in two massive datasets; The second is how to predict the B cell epitopes effectively.
【學(xué)位授予單位】：上海交通大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2007
【分類號】：R392

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