本文關(guān)鍵詞：碳原子線修飾電極對(duì)腎上腺素及尿酸的電催化作用　出處：《南京師范大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 碳原子線 修飾電極 腎上腺素 尿酸

【摘要】：本文報(bào)告碳原子線(carbon atom wires，簡(jiǎn)寫為CAWs)修飾電極對(duì)腎上腺素和尿酸的電催化作用。腎上腺素和尿酸是人體內(nèi)的生理活性物質(zhì)，研究它們的電化學(xué)反應(yīng)對(duì)深入研究生命科學(xué)和醫(yī)藥科學(xué)具有十分重要的意義。 利用循環(huán)伏安法、紫外可見吸收光譜法和強(qiáng)制對(duì)流法研究了腎上腺素在碳原子線修飾電極上的電化學(xué)行為和反應(yīng)機(jī)理。與裸玻碳電極相比，硝酸處理的碳原子線修飾玻碳電極在含有1mmol／L腎上腺素的0.5mol／L H_2SO_4水溶液中的循環(huán)伏安曲線上的氧化峰電位E_(pa)負(fù)移87mV，還原峰電位E_(pc)正移139mV，氧化峰電流i_(pa)和還原峰電流i_(pa)分別增加5.6倍和16.4倍。由強(qiáng)制對(duì)流法測(cè)得，在0.5mol／L H_2SO_4水溶液中、硝酸處理過的碳原子線修飾電極上，腎上腺素氧化成腎上腺素醌的異相電子傳遞標(biāo)準(zhǔn)速率常數(shù)k~0相對(duì)于裸電極增大16倍，這些結(jié)果表明碳原子線對(duì)于腎上腺素的電極過程具有非常高的電催化活性。通過循環(huán)伏安法結(jié)合紫外可見吸收光譜研究了腎上腺素的電極過程的反應(yīng)機(jī)理。結(jié)果表明，腎上腺素的氧化還原過程比較復(fù)雜，在反應(yīng)過程中除了電極上的多步電子轉(zhuǎn)移外，還伴隨有化學(xué)反應(yīng)，并且在一些反應(yīng)步驟中有H~+的參與，因此，，腎上腺素的循環(huán)伏安曲線上的電化學(xué)響應(yīng)與掃描速率及介質(zhì)的pH值密切相關(guān)。碳原子線修飾電極上的腎上腺素的循環(huán)伏安實(shí)驗(yàn)還表明，其氧化還原峰電流與其濃度在1×10~(-4)～1×10~(-2)mol／L范圍內(nèi)成線性關(guān)系，因此碳原子線修飾電極可用于腎上腺素的電分析，檢測(cè)限為2×10~(6)mol／L。 本文利用差分脈沖伏安法研究了尿酸在不同電解質(zhì)溶液中的電化學(xué)行為，顯示碳原子線修飾電極對(duì)尿酸的電化學(xué)過程具有很好的催化作用。此外，本文還研究了pH在碳原子線修飾電極對(duì)尿酸電催化作用過程中的影響，結(jié)果表明，在pH=4～9的范圍內(nèi)，氧化峰電位E_(pa)與pH成線性關(guān)系，其線性方程的斜率表明，在該電極反應(yīng)過程中有相同數(shù)目的質(zhì)子和電子參與電極反應(yīng)。由實(shí)驗(yàn)還測(cè)得，碳原子線修飾電極的氧化峰電流與掃描速率的平方根成正比，說明該電極反應(yīng)受擴(kuò)散控制。
[Abstract]:In this paper, atom wires of carbon atom is reported. The electrocatalytic action of modified electrodes on adrenaline and uric acid. Epinephrine and uric acid are physiological active substances in human body. The study of their electrochemical reactions is of great significance for the further study of life sciences and medical sciences. The electrochemical behavior and reaction mechanism of epinephrine on carbon atomic line modified electrode were studied by cyclic voltammetry, UV-Vis absorption spectroscopy and forced convection. Oxidation peak potential on cyclic voltammetry curves in 0.5 mol / L H _ 2SO _ 4 aqueous solution containing 1 mmol / L epinephrine on nitric acid treated carbon wire modified glassy carbon electrode. Negative shift 87mV. The reduction peak potential (ESP) was positively shifted to 139mV, the oxidation peak current was increased by 5.6 times and the reduction peak current by 16.4 times by forced convection method. In 0.5 mol / L H _ 2SO _ 4 aqueous solution, the carbon atom line modified electrode was treated with nitric acid. The standard rate constant of heterogeneous electron transfer of epinephrine to adrenerone was 16 times higher than that of bare electrode. These results indicate that carbon atomic lines have very high electrocatalytic activity for the electrode process of epinephrine. The reaction mechanism of the electrode process of epinephrine has been studied by cyclic voltammetry and UV-Vis absorption spectra. To show. The redox process of epinephrine is quite complex. In addition to the multi-step electron transfer on the electrode, there are also chemical reactions in the reaction process. The electrochemical response on the cyclic voltammetry curve of epinephrine is closely related to the scanning rate and pH value of the medium, and the cyclic voltammetry of epinephrine on the carbon atom line modified electrode also shows. The redox peak current is linearly related to the concentration in the range of 1 脳 10 ~ (-1) ~ (-2) mol / L, so the carbon atom line modified electrode can be used for the electrical analysis of epinephrine. The detection limit is 2 脳 10 ~ (10) mol 路L ~ (-1). The electrochemical behavior of uric acid in different electrolyte solutions has been studied by differential pulse voltammetry. The results show that the carbon atom line modified electrode has a good catalytic effect on the electrochemical process of uric acid. The effect of pH on the electrocatalysis of uric acid by carbon atomic wire modified electrode was also studied. The results showed that the oxidation peak potential (EPS) was linearly related to pH in the range of pH=4~9. The slope of the linear equation shows that the same number of protons and electrons participate in the electrode reaction. The oxidation peak current of the modified electrode is proportional to the square root of the scanning rate, which indicates that the electrode reaction is controlled by diffusion.
【學(xué)位授予單位】：南京師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R341

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