本文關(guān)鍵詞：乙肝病毒感染性疾病與Fas系統(tǒng)相關(guān)性研究　出處：《青島大學(xué)》2006年碩士論文　論文類型：學(xué)位論文

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【摘要】： 目的通過對(duì)乙型肝炎病毒感染相關(guān)性疾病患者外周血中淋巴細(xì)胞凋亡情況及細(xì)胞膜表面Fas／FasL的檢測(cè)，結(jié)合乙肝病毒感染后患者血清中可溶性Fas／FasL(sFas／sFasL)水平的改變，探討在疾病發(fā)生發(fā)展過程中，淋巴細(xì)胞表面所表達(dá)的Fas／FasL與血清sFas／sFasL之間的變化規(guī)律，期望進(jìn)一步闡明乙肝病毒感染慢性肝細(xì)胞損害致重癥化和肝細(xì)胞癌變的發(fā)病機(jī)制，為預(yù)防和治療慢性乙型重型肝炎和肝細(xì)胞癌提供新的思路。 方法采用流式細(xì)胞技術(shù)檢測(cè)健康人、乙肝病毒攜帶者、慢性乙型重型肝炎患者、乙肝病毒相關(guān)性肝細(xì)胞癌患者外周血淋巴細(xì)胞的凋亡率及細(xì)胞表面表達(dá)Fas／FasL的情況；采用雙抗體夾心法檢測(cè)患者和健康人血清中sFas／sFasL的表達(dá)。 結(jié)果(1)通過流式細(xì)胞技術(shù)檢測(cè)證實(shí)，與正常對(duì)照組、無癥狀乙肝病毒攜帶組相比較，慢性乙型重型肝炎患者、乙肝病毒相關(guān)性肝細(xì)胞癌患者外周血淋巴細(xì)胞的凋亡率顯著增高，差別有顯著性(P＜0.01)。(2)通過流式細(xì)胞技術(shù)檢測(cè)證實(shí)，與正常對(duì)照組、無癥狀乙肝病毒攜帶組相比較，慢性乙型重型肝炎患者、乙肝病毒相關(guān)性肝細(xì)胞癌患者外周血淋巴細(xì)胞表面表達(dá)的Fas顯著增高，差別有統(tǒng)計(jì)學(xué)意義(P＜0.01)；與正常對(duì)照組、無癥狀乙肝病毒攜帶組相比較，慢性乙型重型肝炎患者外周血淋巴細(xì)胞表面表達(dá)的FasL顯著增高，差別有統(tǒng)計(jì)學(xué)意義(P＜0.01)，但是，，乙肝病毒相關(guān)性肝細(xì)胞癌患者外周血淋巴細(xì)胞表面表達(dá)的FasL顯著降低，與其他三組相比較，差別有統(tǒng)計(jì)學(xué)意義(P＜0.01)。(3)雙抗體夾心法證實(shí)，與正常對(duì)照組、無癥狀乙肝病毒攜帶組相比較，慢性乙型重型肝炎患者血清sFas和sFasL含量均顯著增高，差別有顯著性(P＜0.01)；乙肝病毒相關(guān)性肝細(xì)胞癌患者sFas含量明顯增高，sFasL含量顯著降低，差別有統(tǒng)計(jì)學(xué)意義(P＜0.01)。 結(jié)論(1)慢性乙型重型肝炎患者、乙肝病毒相關(guān)性肝細(xì)胞癌患者外周血淋巴細(xì)胞的凋亡率顯著增高。(2)Fas／FasL在慢性乙型重型肝炎患者和乙肝病毒相關(guān)性肝細(xì)胞癌患者外周血淋巴細(xì)胞中的表達(dá)存在明顯差異。(3)sFas和sFasL在慢性乙型重型肝炎及乙肝相關(guān)性肝細(xì)胞癌之間存在明顯差異。(4)Fas／FasL和sFas／sFasL的相互作用在淋巴細(xì)胞的凋亡中發(fā)揮著重要的作用，與慢性乙型重型肝炎、乙肝病毒相關(guān)性肝細(xì)胞癌的發(fā)病密切相關(guān)。
[Abstract]:Objective to detect the infection of hepatitis B virus in patients with diseases associated with apoptosis of lymphocytes in cell membrane and the surface of Fas / FasL, in combination with hepatitis B virus infection after soluble in the serum of patients with Fas / FasL (sFas / sFasL) level change, to explore in the disease process, variation of lymphocyte surface expression Fas / FasL and sFas / sFasL in serum, pathogenesis and further clarify the expectations caused by severe hepatic cell canceration of chronic liver damage in hepatitis B virus infection, to provide new ideas for the prevention and treatment of chronic severe hepatitis and hepatocellular carcinoma.
Methods using flow cytometry to detect hepatitis B virus carriers, healthy people, patients with chronic severe hepatitis B, peripheral blood lymphocyte surface of hepatitis B virus in patients with hepatocellular carcinoma: correlation between the apoptosis and the expression of Fas / FasL; the expression was determined by ELISA in patients and healthy human serum in sFas / sFasL.
Results (1) confirmed by the detection of flow cytometry, and the normal control group, asymptomatic HBV carriers group compared to patients with chronic severe hepatitis B, apoptosis of peripheral blood lymphocytes in patients with hepatitis B virus related hepatocellular carcinoma was significantly increased, the difference was significant (P < 0.01). (2) by detecting confirmed by flow cytometry, and the normal control group, asymptomatic HBV carriers group, chronic severe hepatitis B patients, surface expression of peripheral blood lymphocytes in patients with hepatitis B virus related hepatocellular carcinoma Fas was significantly increased, the difference was statistically significant (P < 0.01); and the normal control group, asymptomatic hepatitis B virus carrying group compared to FasL lymphocyte surface expression in peripheral blood of patients with chronic severe hepatitis B were significantly higher, the difference was statistically significant (P < 0.01). However, patients with hepatitis B virus related hepatocellular carcinoma peripheral blood lymphocytes Cell surface expression of FasL was significantly decreased compared with the other three groups, the difference was statistically significant (P < 0.01). (3) confirmed by double antibody sandwich method, and the normal control group, asymptomatic HBV carriers group, chronic severe hepatitis B patients serum sFas and sFasL levels were significantly increased, the difference is significant (P < 0.01); the content of sFas of hepatitis B virus related hepatocellular carcinoma patients were significantly increased, sFasL content decreased significantly, the difference was statistically significant (P < 0.01).
Conclusion (1) in patients with chronic severe hepatitis B, apoptosis of peripheral blood lymphocytes in patients with hepatitis B virus related hepatocellular carcinoma was significantly increased. (2) Fas / FasL in patients with chronic severe hepatitis B and hepatitis B virus related hepatocellular carcinoma in peripheral blood lymphocytes was significantly different. (3) and sFas there were significant differences in sFasL between chronic hepatitis B and hepatitis B virus associated hepatocellular carcinoma. (4) the interaction between Fas / FasL and sFas / sFasL plays an important role in lymphocyte apoptosis, and chronic severe hepatitis B, closely related to the pathogenesis of hepatitis B virus associated hepatocellular carcinoma.

【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2006
【分類號(hào)】：R373

【參考文獻(xiàn)】

相關(guān)期刊論文 前6條

1 黃守國,孔北華,楊瑞芳,江森;三氧化二砷抑制人卵巢癌裸鼠腹腔轉(zhuǎn)移瘤的形成及其機(jī)制的初步研究[J];癌癥;2002年04期

2 李玲,顧長海,李欣,顏杰,劉俊;慢性乙型肝炎患者PBMCFas表達(dá)和凋亡的檢測(cè)及意義[J];第三軍醫(yī)大學(xué)學(xué)報(bào);2001年06期

3 高德宗,孫靖中;Fas系統(tǒng)與腫瘤治療[J];國外醫(yī)學(xué)(腫瘤學(xué)分冊(cè));2005年05期

4 王希良,朱錫華;FasL與TNF引起凋亡的研究進(jìn)展[J];國外醫(yī)學(xué)(免疫學(xué)分冊(cè));1999年05期

5 吳雪平;Fas系統(tǒng)介導(dǎo)的細(xì)胞凋亡與肝臟疾病[J];國外醫(yī)學(xué)(免疫學(xué)分冊(cè));1999年02期

6 何建方,周建方,戴利成,施柏年,王偉紅,陸群英;慢性乙型肝炎患者治療前后血清sFas和sFasL的變化及其臨床意義[J];檢驗(yàn)醫(yī)學(xué);2005年01期

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