本文關(guān)鍵詞：重組SjP38抗原診斷日本血吸蟲病的研究　出處：《第一軍醫(yī)大學(xué)》2005年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 日本血吸蟲 SjP38 克隆 蛋白表達(dá) 蛋白純化 單克隆抗體 膠體金 表位免疫診斷 時(shí)間分辨免疫熒光分析 免疫層析技術(shù) 早期診斷

【摘要】：血吸蟲病是當(dāng)今最重要的公共衛(wèi)生疾病之一,全世界有2億人口被感染,其中有1.2億患者。血吸蟲病的主要危害在于蟲卵所分泌的可溶性蟲卵抗原(soluble egg antigen, SEA)導(dǎo)致的肉芽腫病變,患者在晚期時(shí)易引起肝脾腫大,門脈高壓和腹水,喪失勞動(dòng)力,甚至失去生命。因此,實(shí)現(xiàn)早期診斷,成為血吸蟲病防治工作的重點(diǎn)。 血吸蟲病常用診斷方法主要有病原學(xué)和免疫學(xué)方法。傳統(tǒng)的病原學(xué)方法有較高特異性但敏感性較差,而且多數(shù)病原學(xué)方法操作繁瑣,不利于普查。相對(duì)而言,免疫學(xué)方法是血吸蟲病診斷的發(fā)展方向,目前存在的主要問(wèn)題是:1) 診斷用抗原、抗體的大批量來(lái)源及其質(zhì)量監(jiān)控問(wèn)題;2) 循環(huán)抗體(CAb)檢測(cè)和循環(huán)抗原(CAg)檢測(cè)各有利弊,臨床方法以檢測(cè)IgG抗體的存在為主,但難以區(qū)分現(xiàn)癥與既往感染;3) 早期診斷的效果不理想;4) 診斷方法的進(jìn)一步標(biāo)準(zhǔn)化、簡(jiǎn)易快速化、產(chǎn)業(yè)化而真正面向基層與現(xiàn)場(chǎng)。 P38蛋白是SEA中分子量約為38kD的分子,大量實(shí)驗(yàn)證明曼氏血吸蟲天然和重組P38蛋白具有良好的免疫原性,具有潛在的抗病免疫和診斷應(yīng)用價(jià)值。在本研究室先前的工作中,周曉紅等通過(guò)可溶性蟲卵(SEA)與感染兔血清Western-blot實(shí)驗(yàn),發(fā)現(xiàn)在38kD左右蛋白能識(shí)別4周血清;表明該蛋白參與早期免疫反應(yīng)。由于天然SjP38蛋白來(lái)源有限,且純化過(guò)程繁瑣。本室已經(jīng)克隆了日本血吸蟲主要蟲卵P38基因(SjP38)的編碼序列,本研究則進(jìn)一步深入研究重組SjP38蛋白(rSjP38)的表達(dá)、純化和rSjP38單克隆抗體的制備及其在血吸蟲病診斷中的應(yīng)用。
[Abstract]:Schistosomiasis is one of the most important public health diseases. 200 million people worldwide are infected with schistosomiasis. Among them, 120 million patients. The main harm of schistosomiasis is the granulomatous lesion caused by soluble egg antigen-secreted by eggs. It is easy to cause hepatosplenomegaly, portal hypertension and ascites, loss of labor force and even loss of life in patients with advanced stage. Therefore, early diagnosis has become the focus of schistosomiasis prevention and control. The common diagnostic methods of schistosomiasis include etiology and immunology. The traditional etiological methods have high specificity but poor sensitivity, and most of the etiological methods are complicated to operate. Relatively speaking, immunological method is the development direction of schistosomiasis diagnosis. The main problems at present are: 1) Antigen for diagnosis, mass source of antibody and its quality control; 2) the detection of circulating antibody and circulating antigen (CAG) have their advantages and disadvantages. The main clinical method is to detect the presence of IgG antibody, but it is difficult to distinguish the present disease from the previous infection. 3) the effect of early diagnosis is not ideal; 4) the further standardization of diagnostic methods, simple and rapid, industrialization and truly oriented to the grass roots and the scene. P38 protein is a molecular weight of about 38 KD in SEA. A large number of experiments have proved that the natural and recombinant P38 protein of Schistosoma mansoni has good immunogenicity. In the previous work of our laboratory, Zhou Xiaohong and so on through the soluble egg sea) and the infection rabbit serum Western-blot experiment. It was found that the protein of 38 KD could recognize the serum for 4 weeks. It is suggested that this protein is involved in the early immune response due to the limited source of natural SjP38 protein. The P38 gene of Schistosoma japonicum has been cloned and the coding sequence of SjP38) has been cloned. In this study, the expression and purification of recombinant SjP38 protein rSjP38, the preparation of monoclonal antibody against rSjP38 and its application in diagnosis of schistosomiasis were further studied.
【學(xué)位授予單位】：第一軍醫(yī)大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2005
【分類號(hào)】：R392

【引證文獻(xiàn)】

相關(guān)期刊論文 前1條

1 李俊立;王昌富;;日本血吸蟲病重組診斷抗原研究進(jìn)展[J];中國(guó)血吸蟲病防治雜志;2013年01期

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本文編號(hào)：1417004